Q2HJ58 (PRPS1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase 1
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase I
Short name=PRS-I

Gene names

Name:

PRPS1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.
Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by magnesium and inorganic phosphate.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.
Subunit structure	Homodimer. The active form is probably a hexamer composed of 3 homodimers By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	5-phosphoribose 1-diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway hypoxanthine biosynthetic process Inferred from electronic annotation. Source: Compara nervous system development Inferred from electronic annotation. Source: Compara nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: Compara ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro urate biosynthetic process Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB ribose phosphate diphosphokinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 318	317	Ribose-phosphate pyrophosphokinase 1		PRO_0000244988
Regions
	Nucleotide binding	96 – 101	6	ATP By similarity
	Region	212 – 227	16	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	128	1	Magnesium Potential
	Metal binding	130	1	Magnesium Potential
	Metal binding	139	1	Magnesium Potential
	Metal binding	143	1	Magnesium Potential
	Binding site	130	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2HJ58 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 46D017E969908BA0	FASTA	318	34,834
	10 20 30 40 50 60 MPNIKIFSGS SHQDLSQKIA DRLGLELGKV VTKKFSNQET CVEIGESVRG EDVYIVQSGC 70 80 90 100 110 120 GEINDNLMEL LIMINACKIA SASRVTAVIP CFPYARQDKK DKSRAPISAK LVANMLSVAG 130 140 150 160 170 180 ADHIITMDLH ASQIQGFFDI PVDNLYAEPA VLKWIRENIS EWRNCTIVSP DAGGAKRVTS 190 200 210 220 230 240 IADRLNVDFA LIHKERKKAN EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG 250 260 270 280 290 300 ATRVYAILTH GIFSGPAISR INNACFEAVV VTNTIPQEDK MKHCSKIQVI DISMILAEAI 310 RRTHNGESVS YLFSHVPL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal medulla and Uterus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC113300 mRNA. Translation: AAI13301.1. BC142427 mRNA. Translation: AAI42428.1.
IPI	IPI00728223.
RefSeq	NP_001039654.1. NM_001046189.2.
UniGene	Bt.63570.
3D structure databases
ProteinModelPortal	Q2HJ58.
ModBase	Search...
Proteomic databases
PaxDb	Q2HJ58.
PRIDE	Q2HJ58.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000026261; ENSBTAP00000026261; ENSBTAG00000019703.
GeneID	781227.
KEGG	bta:781227.
Organism-specific databases
CTD	5631.
Phylogenomic databases
eggNOG	COG0462.
GeneTree	ENSGT00550000074583.
HOGENOM	HOG000210451.
HOVERGEN	HBG001520.
InParanoid	Q2HJ58.
KO	K00948.
OMA	YVQIQES.
OrthoDB	EOG4SQWX5.
Enzyme and pathway databases
UniPathway	UPA00087; UER00172.
Family and domain databases
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase_dom. IPR005946. Rib-P_diPkinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q2HJ58.
NextBio	20924697.

Entry information

Entry name

PRPS1_BOVIN

Accession

Primary (citable) accession number: Q2HJ58
Secondary accession number(s): A5PKB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 68 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program