ID   GLD2_BOVIN              Reviewed;         484 AA.
AC   Q2HJ44; Q1JPF9;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   16-JUN-2009, entry version 19.
DE   RecName: Full=Poly(A) RNA polymerase GLD2;
DE            EC=2.7.7.19;
DE   AltName: Full=PAP-associated domain-containing protein 4;
GN   Name=PAPD4;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=Hereford; TISSUE=Uterus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cytoplasmic poly(A) RNA polymerase that adds successive
CC       AMP monomers to the 3'-end of specific RNAs, forming a poly(A)
CC       tail. In contrast to the canonical nuclear poly(A) RNA polymerase,
CC       it only adds poly(A) to selected cytoplasmic mRNAs (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + RNA(n) = diphosphate + RNA(n+1).
CC   -!- SUBUNIT: Interacts with CPEB1, CPEB2, CPSF1 and PABPC1 (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By
CC       similarity).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q2HJ44-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q2HJ44-2; Sequence=VSP_034322, VSP_034323;
CC         Note=No experimental confirmation available;
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B-like family. GLD2
CC       subfamily.
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DR   EMBL; BT025394; ABF57350.1; -; mRNA.
DR   EMBL; BC113319; AAI13320.1; -; mRNA.
DR   IPI; IPI00702849; -.
DR   IPI; IPI00843674; -.
DR   RefSeq; NP_001039826.1; -.
DR   UniGene; Bt.92095; -.
DR   Ensembl; ENSBTAG00000006751; Bos taurus.
DR   GeneID; 533862; -.
DR   KEGG; bta:533862; -.
DR   HOVERGEN; Q2HJ44; -.
DR   OMA; Q2HJ44; NQKTEAR.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004652; F:polynucleotide adenylyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0043631; P:RNA polyadenylation; ISS:UniProtKB.
DR   InterPro; IPR002058; PAP_assoc.
DR   Pfam; PF03828; PAP_assoc; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; ATP-binding; Cytoplasm; mRNA processing;
KW   Nucleotide-binding; Nucleus; Transferase.
FT   CHAIN         1    484       Poly(A) RNA polymerase GLD2.
FT                                /FTId=PRO_0000341548.
FT   MOTIF        76     92       Nuclear localization signal (Potential).
FT   ACT_SITE    215    215       By similarity.
FT   VAR_SEQ     157    163       SQQILEL -> NKRTLTW (in isoform 2).
FT                                /FTId=VSP_034322.
FT   VAR_SEQ     164    484       Missing (in isoform 2).
FT                                /FTId=VSP_034323.
SQ   SEQUENCE   484 AA;  56051 MW;  EA2345615C844818 CRC64;
     MFPNSILGRP PFTPNHQQHN NFFALSPSLY SHQQLIDAQF SFHNADLSRA VSLQQLTYGN
     VSPIQTSTSP LFRGRKRLSD EKNLPLDGKR QRFHSPHQEP TIVNHIVPLS DERRYSMSPL
     FHTHYVPDIV RCVPPFREIS ILEPREITLP EAKDKLSQQI LELFEACQQQ VSDLKKKELC
     RTELQREIQL LFPQSRLFLV GSSLNGFGTR SSDGDLCLVV KEEPCFFQVN QKTEARHILT
     LVHKHFCTRL SGYIERPQLI RAKVPIVKFR DKVSCVEFDL NVNNIVGIRN TFLLRTYAYL
     ENRVRPLVLV IKKWASHHDI NDASRGTLSS YSLVLMVLHY LQTLPEPILP SIQKIYPESF
     SPSIQLHLVH QAPCNVPPYL SKNESNLGDL LLGFLKYYAT EFDWNSQMIS VREAKAIPRP
     DGIEWRNKYI CVEEPFDGTN TARAVHEKQK FDMIKDQFLK SWHRLKNRKD LNSILPLRAA
     ILKR
//