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Protein

Poly(A) RNA polymerase GLD2

Gene

PAPD4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation (By similarity).By similarity

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi213 – 2131Magnesium or manganese; catalyticBy similarity
Metal bindingi215 – 2151Magnesium or manganese; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase GLD2 (EC:2.7.7.19)
Alternative name(s):
PAP-associated domain-containing protein 4
Gene namesi
Name:PAPD4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 10

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484Poly(A) RNA polymerase GLD2PRO_0000341548Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621PhosphoserineBy similarity
Modified residuei69 – 691PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ2HJ44.

Interactioni

Subunit structurei

Interacts with CPEB1, CPEB2, CPSF1 and PABPC1.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000008885.

Structurei

3D structure databases

ProteinModelPortaliQ2HJ44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini386 – 44055PAP-associatedAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi76 – 9217Nuclear localization signalSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 PAP-associated domain.Curated

Phylogenomic databases

eggNOGiCOG5260.
GeneTreeiENSGT00550000074490.
HOGENOMiHOG000088606.
HOVERGENiHBG097533.
InParanoidiQ2HJ44.
KOiK14079.
OMAiHHEINDA.
OrthoDBiEOG7DRJ2S.
TreeFamiTF315661.

Family and domain databases

InterProiIPR002058. PAP_assoc.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2HJ44-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFPNSILGRP PFTPNHQQHN NFFALSPSLY SHQQLIDAQF SFHNADLSRA
60 70 80 90 100
VSLQQLTYGN VSPIQTSTSP LFRGRKRLSD EKNLPLDGKR QRFHSPHQEP
110 120 130 140 150
TIVNHIVPLS DERRYSMSPL FHTHYVPDIV RCVPPFREIS ILEPREITLP
160 170 180 190 200
EAKDKLSQQI LELFEACQQQ VSDLKKKELC RTELQREIQL LFPQSRLFLV
210 220 230 240 250
GSSLNGFGTR SSDGDLCLVV KEEPCFFQVN QKTEARHILT LVHKHFCTRL
260 270 280 290 300
SGYIERPQLI RAKVPIVKFR DKVSCVEFDL NVNNIVGIRN TFLLRTYAYL
310 320 330 340 350
ENRVRPLVLV IKKWASHHDI NDASRGTLSS YSLVLMVLHY LQTLPEPILP
360 370 380 390 400
SIQKIYPESF SPSIQLHLVH QAPCNVPPYL SKNESNLGDL LLGFLKYYAT
410 420 430 440 450
EFDWNSQMIS VREAKAIPRP DGIEWRNKYI CVEEPFDGTN TARAVHEKQK
460 470 480
FDMIKDQFLK SWHRLKNRKD LNSILPLRAA ILKR
Length:484
Mass (Da):56,051
Last modified:March 21, 2006 - v1
Checksum:iEA2345615C844818
GO
Isoform 2 (identifier: Q2HJ44-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     157-163: SQQILEL → NKRTLTW
     164-484: Missing.

Note: No experimental confirmation available.
Show »
Length:163
Mass (Da):18,919
Checksum:i5B86ED9719BCA532
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei157 – 1637SQQILEL → NKRTLTW in isoform 2. 1 PublicationVSP_034322
Alternative sequencei164 – 484321Missing in isoform 2. 1 PublicationVSP_034323Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT025394 mRNA. Translation: ABF57350.1.
BC113319 mRNA. Translation: AAI13320.1.
RefSeqiNP_001039826.1. NM_001046361.1. [Q2HJ44-1]
XP_005211397.1. XM_005211340.2. [Q2HJ44-1]
XP_005211398.1. XM_005211341.2. [Q2HJ44-1]
XP_005211399.1. XM_005211342.2. [Q2HJ44-1]
UniGeneiBt.92095.

Genome annotation databases

EnsembliENSBTAT00000008885; ENSBTAP00000008885; ENSBTAG00000006751. [Q2HJ44-1]
ENSBTAT00000048886; ENSBTAP00000045850; ENSBTAG00000006751. [Q2HJ44-2]
GeneIDi533862.
KEGGibta:533862.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT025394 mRNA. Translation: ABF57350.1.
BC113319 mRNA. Translation: AAI13320.1.
RefSeqiNP_001039826.1. NM_001046361.1. [Q2HJ44-1]
XP_005211397.1. XM_005211340.2. [Q2HJ44-1]
XP_005211398.1. XM_005211341.2. [Q2HJ44-1]
XP_005211399.1. XM_005211342.2. [Q2HJ44-1]
UniGeneiBt.92095.

3D structure databases

ProteinModelPortaliQ2HJ44.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000008885.

Proteomic databases

PRIDEiQ2HJ44.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000008885; ENSBTAP00000008885; ENSBTAG00000006751. [Q2HJ44-1]
ENSBTAT00000048886; ENSBTAP00000045850; ENSBTAG00000006751. [Q2HJ44-2]
GeneIDi533862.
KEGGibta:533862.

Organism-specific databases

CTDi167153.

Phylogenomic databases

eggNOGiCOG5260.
GeneTreeiENSGT00550000074490.
HOGENOMiHOG000088606.
HOVERGENiHBG097533.
InParanoidiQ2HJ44.
KOiK14079.
OMAiHHEINDA.
OrthoDBiEOG7DRJ2S.
TreeFamiTF315661.

Miscellaneous databases

NextBioi20876175.

Family and domain databases

InterProiIPR002058. PAP_assoc.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiGLD2_BOVIN
AccessioniPrimary (citable) accession number: Q2HJ44
Secondary accession number(s): Q1JPF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 21, 2006
Last modified: June 24, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.