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Q2HJ44 (GLD2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Poly(A) RNA polymerase GLD2

EC=2.7.7.19
Alternative name(s):
PAP-associated domain-containing protein 4
Gene names
Name:PAPD4
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation By similarity.

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor

Magnesium or manganese By similarity.

Subunit structure

Interacts with CPEB1, CPEB2, CPSF1 and PABPC1 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the DNA polymerase type-B-like family. GLD2 subfamily.

Contains 1 PAP-associated domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q2HJ44-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q2HJ44-2)

The sequence of this isoform differs from the canonical sequence as follows:
     157-163: SQQILEL → NKRTLTW
     164-484: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 484484Poly(A) RNA polymerase GLD2
PRO_0000341548

Regions

Domain386 – 44055PAP-associated
Motif76 – 9217Nuclear localization signal Potential

Sites

Metal binding2131Magnesium or manganese; catalytic By similarity
Metal binding2151Magnesium or manganese; catalytic By similarity

Amino acid modifications

Modified residue621Phosphoserine By similarity
Modified residue691Phosphoserine By similarity

Natural variations

Alternative sequence157 – 1637SQQILEL → NKRTLTW in isoform 2.
VSP_034322
Alternative sequence164 – 484321Missing in isoform 2.
VSP_034323

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: EA2345615C844818

FASTA48456,051
        10         20         30         40         50         60 
MFPNSILGRP PFTPNHQQHN NFFALSPSLY SHQQLIDAQF SFHNADLSRA VSLQQLTYGN 

        70         80         90        100        110        120 
VSPIQTSTSP LFRGRKRLSD EKNLPLDGKR QRFHSPHQEP TIVNHIVPLS DERRYSMSPL 

       130        140        150        160        170        180 
FHTHYVPDIV RCVPPFREIS ILEPREITLP EAKDKLSQQI LELFEACQQQ VSDLKKKELC 

       190        200        210        220        230        240 
RTELQREIQL LFPQSRLFLV GSSLNGFGTR SSDGDLCLVV KEEPCFFQVN QKTEARHILT 

       250        260        270        280        290        300 
LVHKHFCTRL SGYIERPQLI RAKVPIVKFR DKVSCVEFDL NVNNIVGIRN TFLLRTYAYL 

       310        320        330        340        350        360 
ENRVRPLVLV IKKWASHHDI NDASRGTLSS YSLVLMVLHY LQTLPEPILP SIQKIYPESF 

       370        380        390        400        410        420 
SPSIQLHLVH QAPCNVPPYL SKNESNLGDL LLGFLKYYAT EFDWNSQMIS VREAKAIPRP 

       430        440        450        460        470        480 
DGIEWRNKYI CVEEPFDGTN TARAVHEKQK FDMIKDQFLK SWHRLKNRKD LNSILPLRAA 


ILKR 

« Hide

Isoform 2 [UniParc].

Checksum: 5B86ED9719BCA532
Show »

FASTA16318,919

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: Hereford.
Tissue: Uterus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT025394 mRNA. Translation: ABF57350.1.
BC113319 mRNA. Translation: AAI13320.1.
RefSeqNP_001039826.1. NM_001046361.1. [Q2HJ44-1]
XP_005211397.1. XM_005211340.1. [Q2HJ44-1]
XP_005211398.1. XM_005211341.1. [Q2HJ44-1]
XP_005211399.1. XM_005211342.1. [Q2HJ44-1]
UniGeneBt.92095.

3D structure databases

ProteinModelPortalQ2HJ44.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ2HJ44.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000008885; ENSBTAP00000008885; ENSBTAG00000006751. [Q2HJ44-1]
ENSBTAT00000048886; ENSBTAP00000045850; ENSBTAG00000006751. [Q2HJ44-2]
GeneID533862.
KEGGbta:533862.

Organism-specific databases

CTD167153.

Phylogenomic databases

eggNOGCOG5260.
GeneTreeENSGT00550000074490.
HOGENOMHOG000088606.
HOVERGENHBG097533.
InParanoidQ2HJ44.
KOK14079.
OMACVEFDLN.
OrthoDBEOG7DRJ2S.
TreeFamTF315661.

Family and domain databases

InterProIPR002058. PAP_assoc.
[Graphical view]
PfamPF03828. PAP_assoc. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20876175.

Entry information

Entry nameGLD2_BOVIN
AccessionPrimary (citable) accession number: Q2HJ44
Secondary accession number(s): Q1JPF9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 21, 2006
Last modified: July 9, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families