Reviewed, UniProtKB/Swiss-Prot Q2HJ44 (GLD2_BOVIN)

Names and origin

Protein names

Recommended name:
    Poly(A) RNA polymerase GLD2
    EC=2.7.7.19
Alternative name(s):
    PAP-associated domain-containing protein 4

Gene names

Name:

PAPD4

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs By similarity.
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Interacts with CPEB1, CPEB2, CPSF1 and PABPC1 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the DNA polymerase type-B-like family. GLD2 subfamily.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Transferase
Gene Ontology (GO)
Biological process	RNA polyadenylation Inferred from sequence or structural similarity. Source: UniProtKB mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q2HJ44-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q2HJ44-2)

The sequence of this isoform differs from the canonical sequence as follows:
     157-163: SQQILEL → NKRTLTW
     164-484: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Poly(A) RNA polymerase GLD2		PRO_0000341548
Regions
	Motif	76 – 92	17	Nuclear localization signal Potential
Sites
	Active site	215	1	By similarity
Natural variations
	Alternative sequence	157 – 163	7	SQQILEL → NKRTLTW in isoform 2.		VSP_034322
	Alternative sequence	164 – 484	321	Missing in isoform 2.		VSP_034323

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: EA2345615C844818	FASTA	484	56,051
	10 20 30 40 50 60 MFPNSILGRP PFTPNHQQHN NFFALSPSLY SHQQLIDAQF SFHNADLSRA VSLQQLTYGN 70 80 90 100 110 120 VSPIQTSTSP LFRGRKRLSD EKNLPLDGKR QRFHSPHQEP TIVNHIVPLS DERRYSMSPL 130 140 150 160 170 180 FHTHYVPDIV RCVPPFREIS ILEPREITLP EAKDKLSQQI LELFEACQQQ VSDLKKKELC 190 200 210 220 230 240 RTELQREIQL LFPQSRLFLV GSSLNGFGTR SSDGDLCLVV KEEPCFFQVN QKTEARHILT 250 260 270 280 290 300 LVHKHFCTRL SGYIERPQLI RAKVPIVKFR DKVSCVEFDL NVNNIVGIRN TFLLRTYAYL 310 320 330 340 350 360 ENRVRPLVLV IKKWASHHDI NDASRGTLSS YSLVLMVLHY LQTLPEPILP SIQKIYPESF 370 380 390 400 410 420 SPSIQLHLVH QAPCNVPPYL SKNESNLGDL LLGFLKYYAT EFDWNSQMIS VREAKAIPRP 430 440 450 460 470 480 DGIEWRNKYI CVEEPFDGTN TARAVHEKQK FDMIKDQFLK SWHRLKNRKD LNSILPLRAA ILKR
	Isoform 2. Checksum: 5B86ED9719BCA532	FASTA	163	18,919
	10 20 30 40 50 60 MFPNSILGRP PFTPNHQQHN NFFALSPSLY SHQQLIDAQF SFHNADLSRA VSLQQLTYGN 70 80 90 100 110 120 VSPIQTSTSP LFRGRKRLSD EKNLPLDGKR QRFHSPHQEP TIVNHIVPLS DERRYSMSPL 130 140 150 160 FHTHYVPDIV RCVPPFREIS ILEPREITLP EAKDKLNKRT LTW

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Hereford. Tissue: Uterus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT025394 mRNA. Translation: ABF57350.1. BC113319 mRNA. Translation: AAI13320.1.
IPI	IPI00702849. IPI00843674.
RefSeq	NP_001039826.1.
UniGene	Bt.92095
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000006751. Bos taurus. [Contig view]
GeneID	533862.
KEGG	bta:533862.
Phylogenomic databases
HOVERGEN	Q2HJ44.
OMA	Q2HJ44. NQKTEAR.
Family and domain databases
InterPro	IPR002058. PAP_assoc. [Graphical view]
Pfam	PF03828. PAP_assoc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLD2_BOVIN

Accession

Primary (citable) accession number: Q2HJ44
Secondary accession number(s): Q1JPF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)