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Q2HJ40 (BACE1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-secretase 1
EC=3.4.23.46
Alternative name(s):
Beta-site amyloid precursor protein cleaving enzyme 1
Short name=Beta-site APP cleaving enzyme 1
Memapsin-2
Membrane-associated aspartic protease 2
Gene names
Name:BACE1
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase By similarity.

Catalytic activity

Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer's amyloid precursor protein.

Enzyme regulation

Inhibited by RTN3 and RTN4 By similarity.

Subunit structure

Monomer. Interacts with GGA1, GGA2 and GGA3. Interacts with RTN3 and RTN4. Interacts with SNX6 By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein. Golgi apparatus › trans-Golgi network By similarity. Endoplasmic reticulum By similarity. Endosome By similarity. Cell surface By similarity. Cytoplasmic vesicle membrane By similarity. Note: Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface By similarity.

Domain

The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP By similarity.

Post-translational modification

Glycosylated By similarity.

Sequence similarities

Belongs to the peptidase A1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Propeptide22 – 4524 By similarity
PRO_0000245802
Chain46 – 501456Beta-secretase 1
PRO_0000245803

Regions

Topological domain22 – 457436Extracellular Potential
Transmembrane458 – 47821Helical; Potential
Topological domain479 – 50123Cytoplasmic Potential
Region479 – 50123Interaction with RTN3 By similarity

Sites

Active site931 By similarity
Active site2891 By similarity

Amino acid modifications

Glycosylation1531N-linked (GlcNAc...) Potential
Glycosylation1721N-linked (GlcNAc...) Potential
Glycosylation2231N-linked (GlcNAc...) Potential
Glycosylation3541N-linked (GlcNAc...) Potential
Disulfide bond216 ↔ 420 By similarity
Disulfide bond278 ↔ 443 By similarity
Disulfide bond330 ↔ 380 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2HJ40 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: 8E5EA730FC91C295

FASTA50155,725
        10         20         30         40         50         60 
MAQALPWLLL WMGSGVLPAH GSQPGIRLPL RSGLGGAPLG LRLPRETDEE SEEPGRRGSF 

        70         80         90        100        110        120 
VEMVDNLRGK SGQGYYVEMT LGSPPQTLNI LVDTGSSNFA VGAAPHPFLH RYYQRQLSST 

       130        140        150        160        170        180 
YRDLRKGVYV PYTQGKWEGE LGTDLVSIPH GPNVTVRANI AAITESDKFF INGSNWEGIL 

       190        200        210        220        230        240 
GLAYAEIARP DDSLEPFFDS LVKQTHVPNL FSLQLCGAGF PLNQSEALAS VGGSMIIGGI 

       250        260        270        280        290        300 
DHSLYMGSLW YTPIRREWYY EVIIVRVEIN GQDLKMDCKE YNYDKSIVDS GTTNLRLPKK 

       310        320        330        340        350        360 
VFEAAVKSIK AASSTEKFPD GFWLGEQLVC WQAGTTPWNI FPVISLYLMG EVTNQSFRIT 

       370        380        390        400        410        420 
ILPQQYLRPV EDVATSQDDC YKFAISQSST GTVMGAVIME GFYVVFDRAR KRIGFAVSAC 

       430        440        450        460        470        480 
HVHDEFRTAA VEGPFVTPDM EDCGYNIPQT DESTLMTIAY VMAAICALFM LPLCLMVCQW 

       490        500 
RCLRCLRHQH DDFADDISLL K

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Uterus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC113325 mRNA. Translation: AAI13326.1.
IPIIPI00691457.
RefSeqNP_001039996.1. NM_001046531.1.
UniGeneBt.28273.

3D structure databases

ProteinModelPortalQ2HJ40.
SMRQ2HJ40. Positions 55-447.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2HJ40.

Protein family/group databases

MEROPSA01.004.

Proteomic databases

PRIDEQ2HJ40.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000040056; ENSBTAP00000039837; ENSBTAG00000019365.
GeneID614333.
KEGGbta:614333.

Organism-specific databases

CTD23621.

Phylogenomic databases

eggNOGmaNOG11594.
GeneTreeENSGT00600000084334.
HOVERGENHBG059578.
InParanoidQ2HJ40.
OMASFVEMVD.
OrthoDBEOG4SF95Q.
PhylomeDBQ2HJ40.

Family and domain databases

InterProIPR009119. Pept_A1_BACE.
IPR009120. Pept_A1_BACE1.
IPR001461. Peptidase_A1.
IPR021109. Peptidase_aspartic.
IPR001969. Peptidase_aspartic_AS.
IPR009007. Peptidase_aspartic_catalytic.
[Graphical view]
Gene3DG3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
KOK04521.
PANTHERPTHR13683. Peptidase_A1. 1 hit.
PfamPF00026. Asp. 1 hit.
[Graphical view]
PRINTSPR01816. BACE1.
PR01815. BACEFAMILY.
PR00792. PEPSIN.
SUPFAMSSF50630. Pept_Aspartic. 1 hit.
PROSITEPS00141. ASP_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBACE1_BOVIN
AccessionPrimary (citable) accession number: Q2HJ40
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 21, 2006
Last modified: November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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