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Protein

Myc proto-oncogene protein

Gene

MYC

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-BTA-69202. Cyclin E associated events during G1/S transition.
R-BTA-69656. Cyclin A:Cdk2-associated events at S phase entry.

Names & Taxonomyi

Protein namesi
Recommended name:
Myc proto-oncogene protein
Alternative name(s):
Proto-oncogene c-Myc
Transcription factor p64
Gene namesi
Name:MYC
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 14

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439Myc proto-oncogene proteinPRO_0000244986Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei6 – 61PhosphoserineBy similarity
Modified residuei58 – 581Phosphothreonine; by GSK3; alternateBy similarity
Glycosylationi58 – 581O-linked (GlcNAc); alternateBy similarity
Modified residuei62 – 621Phosphoserine; by DYRK2, GSK3 and CDK2By similarity
Modified residuei71 – 711PhosphoserineBy similarity
Modified residuei143 – 1431N6-acetyllysine; by PCAFBy similarity
Modified residuei148 – 1481N6-acetyllysineBy similarity
Modified residuei157 – 1571N6-acetyllysine; by PCAFBy similarity
Modified residuei161 – 1611PhosphoserineBy similarity
Modified residuei275 – 2751N6-acetyllysine; by PCAFBy similarity
Modified residuei317 – 3171N6-acetyllysine; by PCAFBy similarity
Modified residuei323 – 3231N6-acetyllysine; by PCAFBy similarity
Modified residuei329 – 3291Phosphoserine; by PIM2; in vitroBy similarity
Modified residuei371 – 3711N6-acetyllysine; by PCAFBy similarity

Post-translational modificationi

Phosphorylated by PRKDC. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome (By similarity). Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC. Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence (By similarity).By similarity
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ2HJ27.
PRIDEiQ2HJ27.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B. Interacts (when phosphorylated at Thr-58 and Ser-62) with FBXW7. Interacts with PIM2. Interacts with NO66. The heterodimer MYC:MAX interacts with ABI1; the interaction may enhance MYC:MAX transcriptional activity. Interacts with TRIM6.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000011066.

Structurei

3D structure databases

ProteinModelPortaliQ2HJ27.
SMRiQ2HJ27. Positions 353-434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini354 – 40653bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni413 – 43422Leucine-zipperAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi33 – 375Poly-Gln
Compositional biasi88 – 914Poly-Gly

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IFSM. Eukaryota.
ENOG41124Q3. LUCA.
GeneTreeiENSGT00510000046414.
HOGENOMiHOG000043075.
HOVERGENiHBG000472.
InParanoidiQ2HJ27.
KOiK04377.
OMAiAASECID.
OrthoDBiEOG7GJ6CX.
TreeFamiTF106001.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003327. Myc-LZ.
IPR002418. Tscrpt_reg_Myc.
IPR012682. Tscrpt_reg_Myc_N.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
PF02344. Myc-LZ. 1 hit.
PF01056. Myc_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001705. Myc_protein. 1 hit.
PRINTSiPR00044. LEUZIPPRMYC.
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2HJ27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLNVSFANK NYDLDYDSVQ PYFYCDEEEN FYHQQQQSEL QPPAPSEDIW
60 70 80 90 100
KKFELLPTPP LSPSRRSGLC SPSYVAVASF SPRGDDDGGG GSFSSADQLE
110 120 130 140 150
MVTELLGGDM VNQSFICDPD DETLIKNIII QDCMWSGFSA AAKLVSEKLA
160 170 180 190 200
SYQAARKDGG SPSPARGHGG CSTSSLYLQD LSAAASECID PSVVFPYPLN
210 220 230 240 250
DSSSPKPCAS PDSTAFSPSS DSLLSSAESS PRASPEPLAL HEETPPTTSS
260 270 280 290 300
DSEEEQEDEE EIDVVSVEKR QPPAKRSESG SPSAGSHSKP PHSPLVLKRC
310 320 330 340 350
HVSTHQHNYA APPSTRKDYP AAKRAKLDSG RVLKQISNNR KCASPRSSDT
360 370 380 390 400
EENDKRRTHN VLERQRRNEL KRSFFALRDQ IPELENNEKA PKVVILKKAT
410 420 430
AYILSVQAEQ QKLKSEIDVL QKRREQLKLK LEQIRNSCA
Length:439
Mass (Da):48,390
Last modified:March 21, 2006 - v1
Checksum:i25845F7A07DF31B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC113343 mRNA. Translation: AAI13344.1.
RefSeqiNP_001039539.1. NM_001046074.2.
XP_005215381.1. XM_005215324.3.
XP_010810231.1. XM_010811929.2.
UniGeneiBt.21164.

Genome annotation databases

EnsembliENSBTAT00000011066; ENSBTAP00000011066; ENSBTAG00000008409.
GeneIDi511077.
KEGGibta:511077.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC113343 mRNA. Translation: AAI13344.1.
RefSeqiNP_001039539.1. NM_001046074.2.
XP_005215381.1. XM_005215324.3.
XP_010810231.1. XM_010811929.2.
UniGeneiBt.21164.

3D structure databases

ProteinModelPortaliQ2HJ27.
SMRiQ2HJ27. Positions 353-434.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000011066.

Proteomic databases

PaxDbiQ2HJ27.
PRIDEiQ2HJ27.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000011066; ENSBTAP00000011066; ENSBTAG00000008409.
GeneIDi511077.
KEGGibta:511077.

Organism-specific databases

CTDi4609.

Phylogenomic databases

eggNOGiENOG410IFSM. Eukaryota.
ENOG41124Q3. LUCA.
GeneTreeiENSGT00510000046414.
HOGENOMiHOG000043075.
HOVERGENiHBG000472.
InParanoidiQ2HJ27.
KOiK04377.
OMAiAASECID.
OrthoDBiEOG7GJ6CX.
TreeFamiTF106001.

Enzyme and pathway databases

ReactomeiR-BTA-69202. Cyclin E associated events during G1/S transition.
R-BTA-69656. Cyclin A:Cdk2-associated events at S phase entry.

Miscellaneous databases

NextBioi20869761.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003327. Myc-LZ.
IPR002418. Tscrpt_reg_Myc.
IPR012682. Tscrpt_reg_Myc_N.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
PF02344. Myc-LZ. 1 hit.
PF01056. Myc_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001705. Myc_protein. 1 hit.
PRINTSiPR00044. LEUZIPPRMYC.
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiMYC_BOVIN
AccessioniPrimary (citable) accession number: Q2HJ27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 21, 2006
Last modified: April 13, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.