Reviewed, UniProtKB/Swiss-Prot Q2HG02 (MCR1_CHAGB)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 2
    EC=1.6.2.2
Alternative name(s):
    Mitochondrial cytochrome b reductase

Gene names

Name:	MCR1
ORF Names:	CHGG_00852

Organism

Chaetomium globosum (Soil fungus) [Complete proteome]

Taxonomic identifier

38033 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Chaetomiaceae › Chaetomium

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	NADH-cytochrome b5 reductase 2		PRO_0000330178
Regions
	Transmembrane	41 – 61	21	Potential
	Domain	97 – 202	106	FAD-binding FR-type
	Nucleotide binding	205 – 240	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2HG02-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 812F98E27939A0E7	FASTA	348	38,131
	10 20 30 40 50 60 MSLFVASRSA FRAAAPLKRQ FQIRRYATEP PSADAKKGNN TLLYGAAAAA VAGAGYYFLG 70 80 90 100 110 120 GTPAAKKAEE KVKDASSAAA GKLSTSEVKQ ALTGGEQGWV SLKLEEVEIV NHNSKRLRFR 130 140 150 160 170 180 LPEDDMVSGV HVASAILTKF KPVDAEKPVI RPYTPTNDED ARGYLDLLVK KYPNGPMSTH 190 200 210 220 230 240 LHDMVPGQRL DVKGPLPKYP WTANKHGHIA LVAGGTGITP MFQLCRAIFN NPDDQTKVTL 250 260 270 280 290 300 VFGNVREDDI LLKKELAALE NNNPRRFRAF YVLDDPPKHW TGGKGFITKD LLKTVLPEPK 310 320 330 340 DENIKVFVCG PPGMMDAISG NKKSPKDQGE LKGILKELGY SPEQVYKF

References

[1]

"Annotation of the Chaetomium globosum CBS 148.51 genome." Birren B.W., Lander E.S., Galagan J.E., Devon K., Nusbaum C., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K., Pushparaj V. , DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Kodira C.D., Yandava C., Zeng Q., Alvarado L., Oleary S., Untereiner W. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 6205 / CBS 148.51 / DSM 1962 / IFO 6347 / NRRL 1970.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH408029 Genomic DNA. Translation: EAQ92617.1.
RefSeq	XP_001220073.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4388100.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1_CHAGB

Accession

Primary (citable) accession number: Q2HG02

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)