Reviewed, UniProtKB/Swiss-Prot Q2HFL8 (PMIP_CHAGB)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase
      Short name=MIP
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase

Gene names

Name:	OCT1
ORF Names:	CHGG_00986

Organism

Chaetomium globosum (Soil fungus) [Complete proteome]

Taxonomic identifier

38033 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Chaetomiaceae › Chaetomium

Protein attributes

Sequence length	778 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 37	37	Mitochondrion Potential
	Chain	38 – 778	741	Mitochondrial intermediate peptidase		PRO_0000338578
Sites
	Active site	558	1	By similarity
	Metal binding	557	1	Zinc; catalytic By similarity
	Metal binding	561	1	Zinc; catalytic By similarity
	Metal binding	564	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2HFL8-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 77933C763AF2F3D9	FASTA	778	87,685
	10 20 30 40 50 60 MIRTVTRPRQ WQRWVYSSCL LQRAVPPAAA RQQPRFTTSH KANHFGYHQP IAPVTHAPIT 70 80 90 100 110 120 REDDFVLRSV FDYPQFWQDF TSVPGGIPVG LFRNSFLKEP RGMLTFAYVS LKKARAVVAK 130 140 150 160 170 180 VLAASSVTEY RLIVRDLDRL SDILCRVLDM ADFVRVTHPD PEIQQNASKA WESVYEYMNE 190 200 210 220 230 240 LNTMTGLHDQ LATAMANPDV TVVWSEEEKT VAEVLKLDFT KSAVSLPQKY RDRFVQLSSE 250 260 270 280 290 300 ISAVGSAFVQ EMAPEQETVV LPSSDLRGMD PVRARDLTRR GKVYLPTLSG EAVMALRTVH 310 320 330 340 350 360 DADARKHIFY ASRTASRRSV QMLEYLMRLR SELASLSGFE SYGHLALRDR MMAKSPEAVD 370 380 390 400 410 420 QFLRALVESN RPKAMQEMAE LLAEKQKAYP QTNSLDPWDK DYYSDIIRRP LRVAGRQGDL 430 440 450 460 470 480 LSSYFSLGVP LVGETWHPDV RRLDVVSDTD GHVAVLYCDL FTRPNKSPNP AHFTLRCSRE 490 500 510 520 530 540 IFASEMEEVW EQTQQSNQKS MFGSPELAAT DGMTFSRHGD SIKQLPTIAL VCDFPQPSKH 550 560 570 580 590 600 GDQPALLSFL QLETLFHEMG HAIHSVLART SFQTVAGTRC ATDLAELPST LMEFFAADAA 610 620 630 640 650 660 VLGQFARHHE TNEPLPYRMV AQKARQTRRF EALDTENQIV TAMFDQALHS PRAGEPGFDP 670 680 690 700 710 720 TAIFHALQRT HSCAPPDPPG TSWPGFFGHL SGYGSVYYSY LFDRVLAQRV WDVVFKAGER 730 740 750 760 770 RAALSRENGE KLKQSLLKWG GARDPWKCLA EALDDDRLAE GGEEAMALVG SWGSTKPR

References

[1]

"Annotation of the Chaetomium globosum CBS 148.51 genome." Birren B.W., Lander E.S., Galagan J.E., Devon K., Nusbaum C., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K., Pushparaj V. , DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Kodira C.D., Yandava C., Zeng Q., Alvarado L., Oleary S., Untereiner W. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 6205 / CBS 148.51 / DSM 1962 / IFO 6347 / NRRL 1970.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH408029 Genomic DNA. Translation: EAQ92751.1.
RefSeq	XP_001220207.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4386396.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. IPR006025. Pept_M_Zn_BS. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_CHAGB

Accession

Primary (citable) accession number: Q2HFL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)