Reviewed, UniProtKB/Swiss-Prot Q2H9L1 (DOT1_CHAGB)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase, H3 lysine-79 specific
    EC=2.1.1.43
Alternative name(s):
    Histone H3-K79 methyltransferase
    H3-K79-HMTase

Gene names

Name:	DOT1
ORF Names:	CHGG_03093

Organism

Chaetomium globosum (Soil fungus) [Complete proteome]

Taxonomic identifier

38033 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Chaetomiaceae › Chaetomium

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Enzyme regulation	Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3 By similarity.
Subcellular location	Nucleus By similarity.
Miscellaneous	In contrast to other lysine histone methyltransferase, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.
Sequence similarities	Belongs to the DOT1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Histone-lysine N-methyltransferase, H3 lysine-79 specific		PRO_0000270608
Regions
	Region	356 – 358	3	S-adenosyl-L-methionine binding By similarity
	Region	416 – 417	2	S-adenosyl-L-methionine binding By similarity
	Motif	352 – 363	12	SAM-binding motif 1 By similarity
	Motif	431 – 440	10	SAM-binding motif 2 By similarity
Sites
	Binding site	334	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	380	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2H9L1-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 1D678E4D39D6A9D4	FASTA	510	56,217
	10 20 30 40 50 60 MSIFNQKSKF KVKTEVRKVK QAVEPTPESK KRYVGNGSAS ASTNGTPRAS PTPSSLQVKR 70 80 90 100 110 120 PRPRPGAGAA SPATGPSLSS STSASPLDLT RKRRAPAGSS SSRSPATASP APRALSDSEP 130 140 150 160 170 180 GSDDDDDDDW RDRLDPSKRR KRAHTEDPDR RLRHPRLWAG QGDEDKPGIV HAVQVASLAD 190 200 210 220 230 240 KCQPVMKLAR DEVGVRLRYP GAKYTERYEL VWGKDKIDGA LDIMKVVKFV ASTYLTDAEA 250 260 270 280 290 300 KPFLDHNLGI NRRLEKSKNT NDGEGFKAAL AEYNNQLLAL QTEGAIARNI DAMRGVPREL 310 320 330 340 350 360 VEFILDQVYD RTVAPKVDLL AKYENGTDNV YGELLHPFIS DIFDRTKLSS DMVFVDLGSG 370 380 390 400 410 420 VGNVTLQAAL ERGCESWGCE MMENACNLAE AQKKEFTARC RMWGIAPGKV HLERGDFRKS 430 440 450 460 470 480 ERTLAALKRA DVVLVNNQAF TSELNDHLVN IFLDLKIGCK IVSLKTFVHD NKIAENDVAS 490 500 510 SILEVEDLRY HEGYVSWTGA AGSFCISTRK

References

[1]

"Annotation of the Chaetomium globosum CBS 148.51 genome." Birren B.W., Lander E.S., Galagan J.E., Devon K., Nusbaum C., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K., Pushparaj V. , DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Kodira C.D., Yandava C., Zeng Q., Alvarado L., Oleary S., Untereiner W. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 6205 / CBS 148.51 / DSM 1962 / IFO 6347 / NRRL 1970.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH408030 Genomic DNA. Translation: EAQ91158.1.
RefSeq	XP_001229609.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4389504.
Enzyme and pathway databases
BRENDA	2.1.1.43. 81575.
Family and domain databases
InterPro	IPR013110. DOT1. [Graphical view]
Pfam	PF08123. DOT1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DOT1_CHAGB

Accession

Primary (citable) accession number: Q2H9L1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)