Q2H6M8 (ATG15_CHAGB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative lipase atg15
EC=3.1.1.3

Alternative name(s):
Autophagy-related protein 15

Gene names

Name:	atg15
ORF Names:	CHGG_05687

Organism

Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) (Soil fungus) [Complete proteome]

Taxonomic identifier

306901 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Chaetomiaceae › Chaetomium

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW multivesicular body membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Putative lipase atg15		PRO_0000317960
Regions
	Topological domain	1 – 9	9	Cytoplasmic By similarity
	Transmembrane	10 – 30	21	Helical; Signal-anchor for type II membrane protein
	Topological domain	31 – 499	469	Lumenal By similarity
	Compositional bias	438 – 462	25	Thr-rich
Sites
	Active site	290	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	170	1	N-linked (GlcNAc...) Potential
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
	Glycosylation	250	1	N-linked (GlcNAc...) Potential
	Glycosylation	274	1	N-linked (GlcNAc...) Potential
	Glycosylation	436	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2H6M8 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 23AD1C9BC1CFC0AD	FASTA	499	54,141
	10 20 30 40 50 60 MSIGEVSDSA GHLASLVLPI EVAPIAPLIP EPPATAEHIF TLRHVYHHGT HKHPSLHRKH 70 80 90 100 110 120 DVTPSNADVW LAAEDGYEAE RIGPLRARSN ALRIQRLVDR RPSVVDPMVA QSRQQGFVSV 130 140 150 160 170 180 LSPSAWTVDD VAGPDVTDKG TVLTMALMAA NAYVEGPGKA DWQDVGAPFN RSLDFGWEGD 190 200 210 220 230 240 GLRGHVFADE NNSTIVIGLK GTSPAVFDGD GTTTNDKVND NLFFSCCCAQ QGPWTWHQVC 250 260 270 280 290 300 DCATGTYTCN NTCVTQALRE ENRYYQAARE LYANVTEVYP DSHVWIAGHS LGGAVSSFLG 310 320 330 340 350 360 LTYGVPVVTF QAVPDALPAG RLGLPVPPGA DPNAPQSRDY TGAFHFGHTA DPIYMGSCNG 370 380 390 400 410 420 ATASCSFAGY ALESACHTGH ECVYDTVGDK GWRVGIGTHK IVAVIRDVIL KYDTVPECKF 430 440 450 460 470 480 TPECRDCGNW KMYESNGTET TTTSSAPTTT SISRTRTETC KTPHLKSQQQ RQRPRPRPLH 490 ALPQWEGGNP DSPERNEEM

References

[1]

"Annotation of the Chaetomium globosum CBS 148.51 genome." The Broad Institute Genome Sequencing Platform Birren B.W., Lander E.S., Galagan J.E., Devon K., Nusbaum C., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K., Pushparaj V. , DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Kodira C.D., Yandava C., Zeng Q., Alvarado L., Oleary S., Untereiner W. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH408031 Genomic DNA. Translation: EAQ89068.1.
RefSeq	XP_001221782.1. XM_001221781.1.
3D structure databases
ProteinModelPortal	Q2H6M8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4390326.
Phylogenomic databases
OrthoDB	EOG4F7RV2.
Family and domain databases
InterPro	IPR002921. Lipase_3. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_CHAGB

Accession

Primary (citable) accession number: Q2H6M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program