Reviewed, UniProtKB/Swiss-Prot Q2H132 (CARA_CHAGB)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase arginine-specific small chain
      Short name=CPS-A
    EC=6.3.5.5
Alternative name(s):
    Arginine-specific carbamoyl-phosphate synthetase, glutamine chain

Gene names

Name:	CPA1
ORF Names:	CHGG_04514

Organism

Chaetomium globosum (Soil fungus) [Complete proteome]

Taxonomic identifier

38033 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Chaetomiaceae › Chaetomium

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1.
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Carbamoyl-phosphate synthase arginine-specific small chain		PRO_0000290592
Regions
	Domain	240 – 427	188	Glutamine amidotransferase type-1
Sites
	Active site	316	1	Nucleophile By similarity
	Active site	400	1	By similarity
	Active site	402	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2H132-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 733A877682E9348A	FASTA	461	50,215
	10 20 30 40 50 60 MSFFFSRQSD ISFLLFSEST STTRNSSQAR LLSRLAVDGS KGRAMPVLNT TRATAAASGV 70 80 90 100 110 120 DATLTIRDGP VFHGTAFGAN SNISGEAVFT TSLVGYPESM TDPSYRGQIL VFTQPLIGNY 130 140 150 160 170 180 GVPSNQRDEY GLLKYFESPH IQCAGVVVAD VAEKYSHWTA VESLSEWCAR EGVPVISGVD 190 200 210 220 230 240 TRAIVTHLRE QGSSLARISI GDEYDADEDE GFIDPGAINL VKRVSTKAPF VVSNPNATFH 250 260 270 280 290 300 VALIDCGVKE NILRSLVSRG ASVTVFPYNY PIHKVADNFD GVFISNGPGD PTHCQETVYN 310 320 330 340 350 360 LGRLMETSSV PIMGICLGHQ LLALAVGAQT IKLKYGNRAH NIPALDLTTG QCHITSQNHG 370 380 390 400 410 420 YAVDASTLPS EFKEYFVNLN DGSNEGMMHK TRPIFSTQFH PEAKGGPMDS SYLFDKYLEN 430 440 450 460 VQMYKDNSKV YRDNRPSQLM IDILSKERVG VEPSPLAANA I

References

[1]

"Annotation of the Chaetomium globosum CBS 148.51 genome." Birren B.W., Lander E.S., Galagan J.E., Devon K., Nusbaum C., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K., Pushparaj V. , DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Kodira C.D., Yandava C., Zeng Q., Alvarado L., Oleary S., Untereiner W. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 6205 / CBS 148.51 / DSM 1962 / IFO 6347 / NRRL 1970.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH408032 Genomic DNA. Translation: EAQ87895.1.
RefSeq	XP_001223728.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4392718.
Enzyme and pathway databases
BRENDA	6.3.5.5. 81575.
Family and domain databases
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_CHAGB

Accession

Primary (citable) accession number: Q2H132

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)