Leukotriene A-4 hydrolase homolog - Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) (Soil fungus)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Leukotriene A-4 hydrolase homolog
Short name=LTA-4 hydrolase
EC=3.3.2.6

Alternative name(s):
Leukotriene A(4) hydrolase

Gene names

ORF Names:

CHGG_07133

Organism

Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) (Soil fungus) [Complete proteome]

Taxonomic identifier

306901 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Chaetomiaceae › Chaetomium ›

Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Aminopeptidase that preferentially cleaves tripeptides. Also has low epoxide hydrolase activity (in vitro). Can hydrolyze an epoxide moiety of LTA₄ to form LTB₄ (in vitro) By similarity.
Catalytic activity	(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H₂O = (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Lipid metabolism; leukotriene B4 biosynthesis.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW peptide catabolic process Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB epoxide hydrolase activity Inferred from sequence or structural similarity. Source: UniProtKB leukotriene-A4 hydrolase activity Inferred from electronic annotation. Source: EC metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 611

611

Leukotriene A-4 hydrolase homolog

PRO_0000324926

Regions

Region

135 – 137

3

Substrate binding By similarity

Region

265 – 270

6

Substrate binding By similarity

Sites

Active site

295

1

Proton acceptor By similarity

Active site

383

1

Proton donor By similarity

Metal binding

294

1

Zinc; catalytic By similarity

Metal binding

298

1

Zinc; catalytic By similarity

Metal binding

317

1

Zinc; catalytic By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q2GY21 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: DCF0315477CB52A3
Show »

FASTA

611

69,072

        10         20         30         40         50         60 
MAPVRDPNTL SNYNEWRTKH TTADFKVDFT AKCLRGSVVL ELESQTDKAS KEIILDSSYV 

        70         80         90        100        110        120 
DVSAITLNST PSQWEVRDRT GPSGSPVRVA VPNGAGKGEV VKLEIELATT DKCTALQWLT 

       130        140        150        160        170        180 
PAQTSNKKAP FMFSQCQAIH ARSIFPCQDT PDVKSTYDFI IRSPHVVVAS GVPVPGEPES 

       190        200        210        220        230        240 
VGEDKVYKFH QKVPIPSYLF AVASGDIASA KIGRCSSVAT GPNELKASQW ELEDDMDKFL 

       250        260        270        280        290        300 
DAAEKIVFPY QWGEYNVLVL PPSFPYGGME NPIFTFATPT IISGDRQNID VIAHELAHSW 

       310        320        330        340        350        360 
SGNLVTSCSW EHFWLNEGWT VYLERRILAS IHKNDSYFDF SAIIGWKHLE EAIEEFGKDH 

       370        380        390        400        410        420 
EYTKLSIKHD GIDPDDAFSS VPYEKGFHFI WSLDRLVGRE NFDKFIPHYF SKWQNKSLDS 

       430        440        450        460        470        480 
FEFKDTFLEF FSAPEYSKLK DKISQIDWEG RFFNPGLPPK PEFDTTLVDG CFQLANKWKS 

       490        500        510        520        530        540 
KDFSPSPSDT SSWTGNQLLV FLNVVQDFEE PLTAEQSQNM GKIYALADSK NVELKAAYYQ 

       550        560        570        580        590        600 
IAMKAKDTTS YPGVAELLGN VGRMKFVRTL FRTLNKVDRD LAVKTFQKNR DFYHPICRQL 

       610 
VEKDLGLGES K

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References

[1]

"Annotation of the Chaetomium globosum CBS 148.51 genome."
The Broad Institute Genome Sequencing Platform
Birren B.W., Lander E.S., Galagan J.E., Devon K., Nusbaum C., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K., Pushparaj V.

Untereiner W.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH408033 Genomic DNA. Translation: EAQ85880.1.
RefSeq	XP_001224789.1. XM_001224788.1.
3D structure databases
ProteinModelPortal	Q2GY21.
ModBase	Search...
Protein family/group databases
MEROPS	M01.034.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4393714.
Phylogenomic databases
OrthoDB	EOG49KJZX.
Enzyme and pathway databases
UniPathway	UPA00878.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR012777. Leukotriene_A4_hydrolase. IPR001930. Peptidase_M1. IPR015211. Peptidase_M1_C. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. PTHR11533. 1 hit.
Pfam	PF09127. Leuk-A4-hydro_C. 1 hit. PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
TIGRFAMs	TIGR02411. leuko_A4_hydro. 1 hit.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LKHA4_CHAGB

Accession

Primary (citable) accession number: Q2GY21

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 21, 2006
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents