Q2GS86 (TRMB_CHAGB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	TRM8
ORF Names:	CHGG_09168

Organism

Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) (Soil fungus) [Complete proteome]

Taxonomic identifier

306901 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Chaetomiaceae › Chaetomium › Chaetomium globosum

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subunit structure	Forms a complex with TRM82 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370593
Regions
	Region	134 – 135	2	S-adenosyl-L-methionine binding By similarity
	Region	169 – 170	2	S-adenosyl-L-methionine binding By similarity
	Region	267 – 269	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	192	1	By similarity
	Binding site	111	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	189	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2GS86 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: B6250E938A788357	FASTA	293	33,667
	10 20 30 40 50 60 MGGDKIKKDK RQKREDYRAA MRKDDISELP RKKFYRQRAH ANPFSDHQLI YPPHPDQMDW 70 80 90 100 110 120 SSLYPHYIVD EPTTTSPPPP PPVPEQTPSE PDLTPLRPKP LSKEVSVADI GCGFGGLLIA 130 140 150 160 170 180 LAPTMPEALI LGLEIRVSVT HFVEDRIKAL RAQNQAAGLY RNVGVLRANT MKFLPNFFRK 190 200 210 220 230 240 AQLEKVFICF PDPHFKIRKH KQRIVSTTLN SEYAYVVKPG GIVYTITDVL DLHEWMVQHF 250 260 270 280 290 DAHPSFERVS EEEQEADPCV AIMRTETEEG KKVERHKGEK HVALFRRLED PAW

References

[1]

"Annotation of the Chaetomium globosum CBS 148.51 genome." The Broad Institute Genome Sequencing Platform Birren B.W., Lander E.S., Galagan J.E., Devon K., Nusbaum C., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K., Pushparaj V. , DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Kodira C.D., Yandava C., Zeng Q., Alvarado L., Oleary S., Untereiner W. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH408034 Genomic DNA. Translation: EAQ85154.1.
RefSeq	XP_001227095.1. XM_001227094.1.
3D structure databases
ProteinModelPortal	Q2GS86.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4395245.
Phylogenomic databases
OrthoDB	EOG4M3DJF.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_CHAGB

Accession

Primary (citable) accession number: Q2GS86

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	March 21, 2006
Last modified:	October 3, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program