Reviewed, UniProtKB/Swiss-Prot Q2GLK4 (SYH_ANAPZ)

Names and origin

Protein names

Recommended name:
    Histidyl-tRNA synthetase
    EC=6.1.1.21
Alternative name(s):
    Histidine--tRNA ligase
      Short name=HisRS

Gene names

Name:	hisS
Ordered Locus Names:	APH_0120

Organism

Anaplasma phagocytophilum (strain HZ) [Complete proteome] [HAMAP]

Taxonomic identifier

212042 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma › phagocytophilum group

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His). HAMAP MF_00127
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP histidine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Histidyl-tRNA synthetase HAMAP MF_00127		PRO_1000016309

Sequences

	Sequence		Length	Mass (Da)
	Q2GLK4-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 9A81EAC823EF94E0	FASTA	420	47,159
	10 20 30 40 50 60 MKVGGFQPVR GTKDLLSEEY YKLFHIQNVA QEIGERFGFM PVQTPIFEFQ EVFCKTLGDS 70 80 90 100 110 120 TDVIGKEMYT FADRGGDVLA LRPEFTAAIV RLLLSEKMQP PARLFTAGPV FRYERPQKCR 130 140 150 160 170 180 QRQFHQINYE CFGAGGSQAD AEIISLAYCV LEVFGLHCDV TLEINSLGSS ECMNAYRASL 190 200 210 220 230 240 LSFFEKYRSE LSEDSRRRLQ TNPLRILDSK DATDKEILST APSIEDFYDA ETRASFEGLK 250 260 270 280 290 300 DHLTNLGIPY AVNRRLVRGL DYYTGTVFEY KTSSLGAQDA IIAGGRYDNL VAAMGGENVP 310 320 330 340 350 360 AIGFAGGVER LAALMSYSRT RKFCVFILPI SEEVVSHAMR ITYEIRRTLS GVQVICDIVT 370 380 390 400 410 420 RLKTGIKRAD RQKADIALIL GDEEVNRNAV SCKNMLTGKQ EEISISNITE YLKAMMAERQ

References

[1]

"Comparative genomics of emerging human ehrlichiosis agents." Dunning Hotopp J.C., Lin M., Madupu R., Crabtree J., Angiuoli S.V., Eisen J.A., Seshadri R., Ren Q., Wu M., Utterback T.R., Smith S., Lewis M., Khouri H., Zhang C., Niu H., Lin Q., Ohashi N., Zhi N. , Nelson W.C., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sundaram J.P., Daugherty S.C., Davidsen T., Durkin A.S., Gwinn M.L., Haft D.H., Selengut J.D., Sullivan S.A., Zafar N., Zhou L., Benahmed F., Forberger H., Halpin R., Mulligan S., Robinson J., White O., Rikihisa Y., Tettelin H. PLoS Genet. 2:208-222(2006) [PubMed: 16482227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000235 Genomic DNA. Translation: ABD44457.1.
RefSeq	YP_504747.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3930760.
GenomeReviews	Gene locus APH_0120 in contig CP000235_GR.
KEGG	aph:APH_0120.
NMPDR	fig|212042.5.peg.117.
TIGR	APH_0120.
Phylogenomic databases
HOGENOM	Q2GLK4.
OMA	Q2GLK4. VFEWVTT.
Enzyme and pathway databases
BioCyc	APHA212042:APH_0120-MON.
Family and domain databases
HAMAP	MF_00127. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR015807. His-tRNA-synth_IIa_sub. IPR004516. His-tRNA_synth_IIA. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
PANTHER	PTHR11476. His-tRNA_synth. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001549. His-tRNA_synth. 1 hit.
TIGRFAMs	TIGR00442. hisS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYH_ANAPZ

Accession

Primary (citable) accession number: Q2GLK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)