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Q2GLB4 (BIOB_ANAPZ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:APH_0218
OrganismAnaplasma phagocytophilum (strain HZ) [Complete proteome] [HAMAP]
Taxonomic identifier212042 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeAnaplasmaphagocytophilum group

Protein attributes

Sequence length322 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 322322Biotin synthase HAMAP-Rule MF_01694
PRO_0000381200

Sites

Metal binding521Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding961Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1271Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1871Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2591Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2GLB4 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: FC501843B43CFA47

FASTA32235,830
        10         20         30         40         50         60 
MIRNNWTLEE TLELFGLPFN DLLFKAHMVH REYFRHNEVQ VCALANIKAG GCPENCCYCS 

        70         80         90        100        110        120 
QSAHNKTGLT KKGLSSVEEV RTAARRAKDV GVHRFCFAAA WRNLHDRDVD RICEFVKAIK 

       130        140        150        160        170        180 
EEGLESCASL GMLKLEQAQK LKEAGLDFYN HNVDTSRAYY HNIVTTRTYD ERLDTINNVQ 

       190        200        210        220        230        240 
EAGINVCCGG ILGMGESTED RAQMLLTLAN LKQHPRSVPI NRLVAFKGTP LEGAKKVNSV 

       250        260        270        280        290        300 
DFVRTIAVAR IMMPASYVRL AAGRSDMTEE MHALCFFAGA NSIFYGEQLL TTENATPEFD 

       310        320 
QNLLSRLGVV ATPSGAHESC AC 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000235 Genomic DNA. Translation: ABD44282.1.
RefSeqYP_504837.1. NC_007797.1.

3D structure databases

ProteinModelPortalQ2GLB4.
SMRQ2GLB4. Positions 3-310.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING212042.APH_0218.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD44282; ABD44282; APH_0218.
GeneID3930883.
KEGGaph:APH_0218.
PATRIC20949010. VBIAnaPha602_0229.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycAPHA212042:GHPM-217-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_ANAPZ
AccessionPrimary (citable) accession number: Q2GLB4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 21, 2006
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways