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Q2GLB4

- BIOB_ANAPZ

UniProt

Q2GLB4 - BIOB_ANAPZ

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Protein
Biotin synthase
Gene
bioB, APH_0218
Organism
Anaplasma phagocytophilum (strain HZ)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi52 – 521Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi96 – 961Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi127 – 1271Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi187 – 1871Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi259 – 2591Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAPHA212042:GHPM-217-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:APH_0218
OrganismiAnaplasma phagocytophilum (strain HZ)
Taxonomic identifieri212042 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeAnaplasmaphagocytophilum group
ProteomesiUP000001943: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Biotin synthaseUniRule annotation
PRO_0000381200Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi212042.APH_0218.

Structurei

3D structure databases

ProteinModelPortaliQ2GLB4.
SMRiQ2GLB4. Positions 3-310.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2GLB4-1 [UniParc]FASTAAdd to Basket

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MIRNNWTLEE TLELFGLPFN DLLFKAHMVH REYFRHNEVQ VCALANIKAG    50
GCPENCCYCS QSAHNKTGLT KKGLSSVEEV RTAARRAKDV GVHRFCFAAA 100
WRNLHDRDVD RICEFVKAIK EEGLESCASL GMLKLEQAQK LKEAGLDFYN 150
HNVDTSRAYY HNIVTTRTYD ERLDTINNVQ EAGINVCCGG ILGMGESTED 200
RAQMLLTLAN LKQHPRSVPI NRLVAFKGTP LEGAKKVNSV DFVRTIAVAR 250
IMMPASYVRL AAGRSDMTEE MHALCFFAGA NSIFYGEQLL TTENATPEFD 300
QNLLSRLGVV ATPSGAHESC AC 322
Length:322
Mass (Da):35,830
Last modified:March 21, 2006 - v1
Checksum:iFC501843B43CFA47
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000235 Genomic DNA. Translation: ABD44282.1.
RefSeqiYP_504837.1. NC_007797.1.

Genome annotation databases

EnsemblBacteriaiABD44282; ABD44282; APH_0218.
GeneIDi3930883.
KEGGiaph:APH_0218.
PATRICi20949010. VBIAnaPha602_0229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000235 Genomic DNA. Translation: ABD44282.1 .
RefSeqi YP_504837.1. NC_007797.1.

3D structure databases

ProteinModelPortali Q2GLB4.
SMRi Q2GLB4. Positions 3-310.
ModBasei Search...

Protein-protein interaction databases

STRINGi 212042.APH_0218.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD44282 ; ABD44282 ; APH_0218 .
GeneIDi 3930883.
KEGGi aph:APH_0218.
PATRICi 20949010. VBIAnaPha602_0229.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci APHA212042:GHPM-217-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HZ.

Entry informationi

Entry nameiBIOB_ANAPZ
AccessioniPrimary (citable) accession number: Q2GLB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 21, 2006
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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