ID   TRUA_ANAPZ              Reviewed;         246 AA.
AC   Q2GKK4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   16-JUN-2009, entry version 24.
DE   RecName: Full=tRNA pseudouridine synthase A;
DE            EC=5.4.99.12;
DE   AltName: Full=tRNA-uridine isomerase I;
DE   AltName: Full=tRNA pseudouridylate synthase I;
GN   Name=truA; OrderedLocusNames=APH_0502;
OS   Anaplasma phagocytophilum (strain HZ).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Anaplasmataceae; Anaplasma; phagocytophilum group.
OX   NCBI_TaxID=212042;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16482227; DOI=10.1371/journal.pgen.0020021;
RA   Dunning Hotopp J.C., Lin M., Madupu R., Crabtree J., Angiuoli S.V.,
RA   Eisen J.A., Seshadri R., Ren Q., Wu M., Utterback T.R., Smith S.,
RA   Lewis M., Khouri H., Zhang C., Niu H., Lin Q., Ohashi N., Zhi N.,
RA   Nelson W.C., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sundaram J.P.,
RA   Daugherty S.C., Davidsen T., Durkin A.S., Gwinn M.L., Haft D.H.,
RA   Selengut J.D., Sullivan S.A., Zafar N., Zhou L., Benahmed F.,
RA   Forberger H., Halpin R., Mulligan S., Robinson J., White O.,
RA   Rikihisa Y., Tettelin H.;
RT   "Comparative genomics of emerging human ehrlichiosis agents.";
RL   PLoS Genet. 2:208-222(2006).
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC       the anticodon stem and loop of transfer RNAs (By similarity).
CC   -!- CATALYTIC ACTIVITY: tRNA uridine = tRNA pseudouridine.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase truA
CC       family.
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DR   EMBL; CP000235; ABD44026.1; -; Genomic_DNA.
DR   RefSeq; YP_505097.1; -.
DR   GeneID; 3931158; -.
DR   GenomeReviews; CP000235_GR; APH_0502.
DR   KEGG; aph:APH_0502; -.
DR   NMPDR; fig|212042.5.peg.496; -.
DR   TIGR; APH_0502; -.
DR   HOGENOM; Q2GKK4; -.
DR   OMA; Q2GKK4; WDPFRLA.
DR   BioCyc; APHA212042:APH_0502-MON; -.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP.
DR   HAMAP; MF_00171; -; 1.
DR   InterPro; IPR001406; tRNA_psdUrid_synth.
DR   Gene3D; G3DSA:3.30.70.580; PseudoU_synth_1; 1.
DR   Gene3D; G3DSA:3.30.70.660; PseudoU_synth_1; 1.
DR   PANTHER; PTHR11142; PseudoU_synth_1; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Isomerase; tRNA processing.
FT   CHAIN         1    246       tRNA pseudouridine synthase A.
FT                                /FTId=PRO_1000017037.
FT   ACT_SITE     53     53       Nucleophile (By similarity).
SQ   SEQUENCE   246 AA;  28030 MW;  33A653101D8CA575 CRC64;
     MRYKAVVEYE GTSFIGWQRQ HGVVGKSVQE SIERSIKEFC QQSVIVYAAG RTDAGVHATG
     QVVHFDLKVS REDYVIKNAL NHYLRNEKVS ILQLERVDEE FHARFSAKKR HYTYKISNRE
     APLCIDLMRM WHVPKRLNVE NMREAASYIV GKHDFASFRA KDCQSKSSIK TVDSIDIIAA
     EEEININISA QSFLHKQVRI TVGTFVECGM GLYPPAHVLE ILEKKDRAAA GITAPAHGLY
     LTRVDY
//