Reviewed, UniProtKB/Swiss-Prot Q2GKK4 (TRUA_ANAPZ)

Names and origin

Protein names

Recommended name:
    tRNA pseudouridine synthase A
    EC=5.4.99.12
Alternative name(s):
    tRNA-uridine isomerase I
    tRNA pseudouridylate synthase I

Gene names

Name:	truA
Ordered Locus Names:	APH_0502

Organism

Anaplasma phagocytophilum (strain HZ) [Complete proteome] [HAMAP]

Taxonomic identifier

212042 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma › phagocytophilum group

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity.
Catalytic activity	tRNA uridine = tRNA pseudouridine. HAMAP MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase truA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	tRNA pseudouridine synthase A HAMAP MF_00171		PRO_1000017037
Sites
	Active site	53	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2GKK4-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 33A653101D8CA575	FASTA	246	28,030
	10 20 30 40 50 60 MRYKAVVEYE GTSFIGWQRQ HGVVGKSVQE SIERSIKEFC QQSVIVYAAG RTDAGVHATG 70 80 90 100 110 120 QVVHFDLKVS REDYVIKNAL NHYLRNEKVS ILQLERVDEE FHARFSAKKR HYTYKISNRE 130 140 150 160 170 180 APLCIDLMRM WHVPKRLNVE NMREAASYIV GKHDFASFRA KDCQSKSSIK TVDSIDIIAA 190 200 210 220 230 240 EEEININISA QSFLHKQVRI TVGTFVECGM GLYPPAHVLE ILEKKDRAAA GITAPAHGLY LTRVDY

References

[1]

"Comparative genomics of emerging human ehrlichiosis agents." Dunning Hotopp J.C., Lin M., Madupu R., Crabtree J., Angiuoli S.V., Eisen J.A., Seshadri R., Ren Q., Wu M., Utterback T.R., Smith S., Lewis M., Khouri H., Zhang C., Niu H., Lin Q., Ohashi N., Zhi N. , Nelson W.C., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sundaram J.P., Daugherty S.C., Davidsen T., Durkin A.S., Gwinn M.L., Haft D.H., Selengut J.D., Sullivan S.A., Zafar N., Zhou L., Benahmed F., Forberger H., Halpin R., Mulligan S., Robinson J., White O., Rikihisa Y., Tettelin H. PLoS Genet. 2:208-222(2006) [PubMed: 16482227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000235 Genomic DNA. Translation: ABD44026.1.
RefSeq	YP_505097.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3931158.
GenomeReviews	Gene locus APH_0502 in contig CP000235_GR.
KEGG	aph:APH_0502.
NMPDR	fig|212042.5.peg.496.
TIGR	APH_0502.
Phylogenomic databases
HOGENOM	Q2GKK4.
OMA	Q2GKK4. WDPFRLA.
Enzyme and pathway databases
BioCyc	APHA212042:APH_0502-MON.
Family and domain databases
HAMAP	MF_00171. [Tree]
InterPro	IPR001406. tRNA_psdUrid_synth. [Graphical view]
Gene3D	G3DSA:3.30.70.580. PseudoU_synth_1. 1 hit. G3DSA:3.30.70.660. PseudoU_synth_1. 1 hit.
PANTHER	PTHR11142. PseudoU_synth_1. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_ANAPZ

Accession

Primary (citable) accession number: Q2GKK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)