Reviewed, UniProtKB/Swiss-Prot Q2GK85 (MDH_ANAPZ)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase
    EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	APH_0629

Organism

Anaplasma phagocytophilum (strain HZ) [Complete proteome] [HAMAP]

Taxonomic identifier

212042 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma › phagocytophilum group

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD(+) = oxaloacetate + NADH.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Malate dehydrogenase		PRO_0000241941
Regions
	Nucleotide binding	13 – 18	6	NAD By similarity
	Nucleotide binding	123 – 125	3	NAD By similarity
Sites
	Active site	180	1	Proton acceptor By similarity
	Binding site	38	1	NAD By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	100	1	NAD By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2GK85-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: DA06315D62C004C8	FASTA	321	33,835
	10 20 30 40 50 60 MRSHRSVKVS LVGAGNIGGT LAYMLGVAGI CQELVFVDVM DGVPRGKLLD IGHALAISGV 70 80 90 100 110 120 DITAVGGSDY AAIEGSDAIV VTAGLPRKEG MSREDLLMAN AAVIKGVAEN IRKYSPDAFV 130 140 150 160 170 180 IVVTNPLDAM VWYMHQCSGL PVNKVVGMAG VLDSARFSFF LAKHMSVSVS SVSSVVLGGH 190 200 210 220 230 240 GDLMLPLLKY STVGGVSVSD LISCGRLSSE DVHAIIERTR KGGEEIVKLL KSGSAYYAPA 250 260 270 280 290 300 ASCMNMLESY LFDKRCVIPC SVGLDGKYGV NGGLFVGVPA VIGKNGVEEV IEYVLSQEER 310 320 EIFEKSVGLI SNSVKIISEQ K

References

[1]

"Comparative genomics of emerging human ehrlichiosis agents." Dunning Hotopp J.C., Lin M., Madupu R., Crabtree J., Angiuoli S.V., Eisen J.A., Seshadri R., Ren Q., Wu M., Utterback T.R., Smith S., Lewis M., Khouri H., Zhang C., Niu H., Lin Q., Ohashi N., Zhi N. , Nelson W.C., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sundaram J.P., Daugherty S.C., Davidsen T., Durkin A.S., Gwinn M.L., Haft D.H., Selengut J.D., Sullivan S.A., Zafar N., Zhou L., Benahmed F., Forberger H., Halpin R., Mulligan S., Robinson J., White O., Rikihisa Y., Tettelin H. PLoS Genet. 2:208-222(2006) [PubMed: 16482227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000235 Genomic DNA. Translation: ABD44326.1.
RefSeq	YP_505216.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3930971.
GenomeReviews	Gene locus APH_0629 in contig CP000235_GR.
KEGG	aph:APH_0629.
NMPDR	fig|212042.5.peg.616.
TIGR	APH_0629.
Phylogenomic databases
HOGENOM	Q2GK85.
Enzyme and pathway databases
BioCyc	APHA212042:APH_0629-MON.
Family and domain databases
HAMAP	MF_00487. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR011275. Malate_DHase_NAD-dep_bac. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_ANAPZ

Accession

Primary (citable) accession number: Q2GK85

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 21, 2006
Last modified:	November 25, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)