Reviewed, UniProtKB/Swiss-Prot Q2GK70 (SYP_ANAPZ)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	APH_0647

Organism

Anaplasma phagocytophilum (strain HZ) [Complete proteome] [HAMAP]

Taxonomic identifier

212042 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma › phagocytophilum group

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01570
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Prolyl-tRNA synthetase HAMAP MF_01570		PRO_0000248888

Sequences

	Sequence		Length	Mass (Da)
	Q2GK70-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 28B518077C79FDC2	FASTA	426	48,223
	10 20 30 40 50 60 MRLSEFYSPT VKNVSSDVVS ASHKYSIRAG IVSQTASGIY TLLPLGLMVL RKVENIIREE 70 80 90 100 110 120 INAVGFSEIL MPTMQPADLW KESQRYDSYG QELIRIHDRG GREMVLGPTH EEVVTDLVRS 130 140 150 160 170 180 SLKSYRDLPV NLYQIQWKFR DELRPRNGIL RSREFLMMDA YSFDTDFEKA MKTYDAVFRA 190 200 210 220 230 240 YRKAFKRMNL QTIALKADMG AIGGSVSHEF HVLTPTGEST VYYDERALEL SEMNDYGIEE 250 260 270 280 290 300 LKEVYAATDD MHDEKSCGIA PEDLKTARGI EVGHIFYLDD RYSRTMNVKF CNTDGHSGTH 310 320 330 340 350 360 VKMGCYGIGI SRLIGALIEV FHDDAGIKWP LSVAPFKVGI VNLFSKNEEC KRVSERIHSV 370 380 390 400 410 420 LPNDSLYDDR DDTPGVKLSR MDLIGLPWQV IVGNSFIKDG VLELKNRATG DIELLSVDDV VSRISV

References

[1]

"Comparative genomics of emerging human ehrlichiosis agents." Dunning Hotopp J.C., Lin M., Madupu R., Crabtree J., Angiuoli S.V., Eisen J.A., Seshadri R., Ren Q., Wu M., Utterback T.R., Smith S., Lewis M., Khouri H., Zhang C., Niu H., Lin Q., Ohashi N., Zhi N. , Nelson W.C., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sundaram J.P., Daugherty S.C., Davidsen T., Durkin A.S., Gwinn M.L., Haft D.H., Selengut J.D., Sullivan S.A., Zafar N., Zhou L., Benahmed F., Forberger H., Halpin R., Mulligan S., Robinson J., White O., Rikihisa Y., Tettelin H. PLoS Genet. 2:208-222(2006) [PubMed: 16482227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000235 Genomic DNA. Translation: ABD43602.1.
RefSeq	YP_505231.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3930859.
GenomeReviews	Gene locus APH_0647 in contig CP000235_GR.
KEGG	aph:APH_0647.
NMPDR	fig|212042.5.peg.632.
TIGR	APH_0647.
Phylogenomic databases
HOGENOM	Q2GK70.
OMA	Q2GK70. VVSHQLM.
Enzyme and pathway databases
BioCyc	APHA212042:APH_0647-MON.
Family and domain databases
HAMAP	MF_01570. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ANAPZ

Accession

Primary (citable) accession number: Q2GK70

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)