Reviewed, UniProtKB/Swiss-Prot Q2GJ36 (SYA_ANAPZ)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	APH_1059

Organism

Anaplasma phagocytophilum (strain HZ) [Complete proteome] [HAMAP]

Taxonomic identifier

212042 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma › phagocytophilum group

Protein attributes

Sequence length	876 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 876	876	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347490

Sequences

	Sequence		Length	Mass (Da)
	Q2GJ36-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 93320B0A64AC0E1E	FASTA	876	96,764
	10 20 30 40 50 60 MSSSSVSGIR EAFLDFFEKQ GHTRYPSAPL IAEGDASLLF TNAGMVPFKQ RFITGVSDVK 70 80 90 100 110 120 TATTSQKCLR AGGKHNDLEN VGYTNRHHTF FEMLGNFSFG DYFKETAIEL AWRFVTKELG 130 140 150 160 170 180 LSKERLWITV YSEDQEAFDI WKKITGYTDH KIIRISTSDN FWSMGDTGPC GPCSEIFYDY 190 200 210 220 230 240 GDGVPGGLPG TDESDGARYT EIWNLVFMQY NRDESGELHK LPRGCIDTGM GLERIAAVMQ 250 260 270 280 290 300 GVCDNYETDM FQAIIDRSRS IFGSHDHPIA HRVIADHVRA ASFLIAEGLT PGNEGRNYVL 310 320 330 340 350 360 RRIIRRAVRY IYQIVGDKFS LHEVVPVLTR EGSAGYMGNA YPEIVKAEQS IVSTLKIEED 370 380 390 400 410 420 GFADTLRRGT GILEQEIRGL KSGEVLSGEI AFKLYDTYGF PLDITLDVAK ERGLKFDEDG 430 440 450 460 470 480 FNRCMAKQKE QSRKHWKGSG EAQTASSHIL NKHKATSFVG YENHRVKSMV KEIFCSGEAV 490 500 510 520 530 540 TSMGEGEEGI AVLDITPFYA ESGGQEGDTG LLKVVTTKCG SVAEVVDTTK SNNVHLHKIR 550 560 570 580 590 600 VIRGTLKVGD VVEAVVDKQR REKLRANHSA THILQSVLRT LIGEGIQQKG SLVAADKLRF 610 620 630 640 650 660 DFSHALPLTK EQLRTVEMEV NRQIMANQPV IIDHCSLEDA VQEGAIALFG EKYNDQNVRV 670 680 690 700 710 720 VSMGSSKELC GGTHVRFTGD IGAFRIISET GIAQGVRRIE AITGHEVVSS MNRDSESLQQ 730 740 750 760 770 780 VAECLSVPVD QVIEKLKKVF VEQREINKKM ATICYTHMNS CAKCIEVGSE IKLYVGEFSN 790 800 810 820 830 840 IPVEVVASYV KEKMHTNEVL AISTTDGKRT TFIVGVGESA IKRIKATDIV KALQQIQGKG 850 860 870 GGNASIARAS LPSEYSAKAA EIIRQTVIDA IQNSGA

References

[1]

"Comparative genomics of emerging human ehrlichiosis agents." Dunning Hotopp J.C., Lin M., Madupu R., Crabtree J., Angiuoli S.V., Eisen J.A., Seshadri R., Ren Q., Wu M., Utterback T.R., Smith S., Lewis M., Khouri H., Zhang C., Niu H., Lin Q., Ohashi N., Zhi N. , Nelson W.C., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sundaram J.P., Daugherty S.C., Davidsen T., Durkin A.S., Gwinn M.L., Haft D.H., Selengut J.D., Sullivan S.A., Zafar N., Zhou L., Benahmed F., Forberger H., Halpin R., Mulligan S., Robinson J., White O., Rikihisa Y., Tettelin H. PLoS Genet. 2:208-222(2006) [PubMed: 16482227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000235 Genomic DNA. Translation: ABD44304.1.
RefSeq	YP_505615.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3930808.
GenomeReviews	Gene locus APH_1059 in contig CP000235_GR.
KEGG	aph:APH_1059.
NMPDR	fig|212042.5.peg.1003.
TIGR	APH_1059.
Phylogenomic databases
HOGENOM	Q2GJ36.
OMA	Q2GJ36. GEFVIKL.
Enzyme and pathway databases
BioCyc	APHA212042:APH_1059-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ANAPZ

Accession

Primary (citable) accession number: Q2GJ36

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)