Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q2GHB1 (BIOB_EHRCR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:ECH_0352
OrganismEhrlichia chaffeensis (strain ATCC CRL-10679 / Arkansas) [Complete proteome] [HAMAP]
Taxonomic identifier205920 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326Biotin synthase HAMAP-Rule MF_01694
PRO_0000381375

Sites

Metal binding571Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding641Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1011Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1321Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1921Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2641Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2GHB1 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: A5DD8AF82730369A

FASTA32636,793
        10         20         30         40         50         60 
MNTCTTIRNN WQLDEILELF NTPFNDLILR SHLTHRQFFN NNEIQLAALL NIKTGGCPEN 

        70         80         90        100        110        120 
CRYCSQSAHY KTDLQKEALL DVENIKKAIQ TAKNSGADRF CFAAAWRQLR DKDIEYICNI 

       130        140        150        160        170        180 
INLIKSEKLE SCASLGMITL DQAKKLKNAG LDFYNHNIDT SRDFYSNVTT TRNYDDRLAS 

       190        200        210        220        230        240 
LNNIYEAGIN ICSGGILGLG ESIEDRAKML LTLANLKEHP RSVPINRLVP IKGTPFENNI 

       250        260        270        280        290        300 
KVDNIDFIKT IAVTRILMPK SYVRLAAGRK DMSEEMQALC LFAGANSIFY GEKLLTTPNS 

       310        320 
NCDDDKNLLS KLGIKTKEPV LLNSQN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000236 Genomic DNA. Translation: ABD45247.1.
RefSeqYP_507172.1. NC_007799.1.

3D structure databases

ProteinModelPortalQ2GHB1.
SMRQ2GHB1. Positions 8-318.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING205920.ECH_0352.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD45247; ABD45247; ECH_0352.
GeneID3927903.
KEGGech:ECH_0352.
PATRIC20576199. VBIEhrCha103583_0300.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMADETQALC.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycECHA205920:GJNR-353-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_EHRCR
AccessionPrimary (citable) accession number: Q2GHB1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 21, 2006
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways