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Q2GHB1

- BIOB_EHRCR

UniProt

Q2GHB1 - BIOB_EHRCR

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Protein
Biotin synthase
Gene
bioB, ECH_0352
Organism
Ehrlichia chaffeensis (strain ATCC CRL-10679 / Arkansas)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi101 – 1011Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi132 – 1321Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi192 – 1921Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi264 – 2641Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciECHA205920:GJNR-353-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:ECH_0352
OrganismiEhrlichia chaffeensis (strain ATCC CRL-10679 / Arkansas)
Taxonomic identifieri205920 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
ProteomesiUP000008320: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Biotin synthaseUniRule annotation
PRO_0000381375Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi205920.ECH_0352.

Structurei

3D structure databases

ProteinModelPortaliQ2GHB1.
SMRiQ2GHB1. Positions 8-318.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2GHB1-1 [UniParc]FASTAAdd to Basket

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MNTCTTIRNN WQLDEILELF NTPFNDLILR SHLTHRQFFN NNEIQLAALL    50
NIKTGGCPEN CRYCSQSAHY KTDLQKEALL DVENIKKAIQ TAKNSGADRF 100
CFAAAWRQLR DKDIEYICNI INLIKSEKLE SCASLGMITL DQAKKLKNAG 150
LDFYNHNIDT SRDFYSNVTT TRNYDDRLAS LNNIYEAGIN ICSGGILGLG 200
ESIEDRAKML LTLANLKEHP RSVPINRLVP IKGTPFENNI KVDNIDFIKT 250
IAVTRILMPK SYVRLAAGRK DMSEEMQALC LFAGANSIFY GEKLLTTPNS 300
NCDDDKNLLS KLGIKTKEPV LLNSQN 326
Length:326
Mass (Da):36,793
Last modified:March 21, 2006 - v1
Checksum:iA5DD8AF82730369A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000236 Genomic DNA. Translation: ABD45247.1.
RefSeqiYP_507172.1. NC_007799.1.

Genome annotation databases

EnsemblBacteriaiABD45247; ABD45247; ECH_0352.
GeneIDi3927903.
KEGGiech:ECH_0352.
PATRICi20576199. VBIEhrCha103583_0300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000236 Genomic DNA. Translation: ABD45247.1 .
RefSeqi YP_507172.1. NC_007799.1.

3D structure databases

ProteinModelPortali Q2GHB1.
SMRi Q2GHB1. Positions 8-318.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 205920.ECH_0352.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD45247 ; ABD45247 ; ECH_0352 .
GeneIDi 3927903.
KEGGi ech:ECH_0352.
PATRICi 20576199. VBIEhrCha103583_0300.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci ECHA205920:GJNR-353-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC CRL-10679 / Arkansas.

Entry informationi

Entry nameiBIOB_EHRCR
AccessioniPrimary (citable) accession number: Q2GHB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 21, 2006
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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