Reviewed, UniProtKB/Swiss-Prot Q2GGL8 (SYE2_EHRCR)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 2
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 2
      Short name=GluRS 2

Gene names

Name:	gltX2
Synonyms:	gltX-2
Ordered Locus Names:	ECH_0605

Organism

Ehrlichia chaffeensis (strain Arkansas) [Complete proteome] [HAMAP]

Taxonomic identifier

205920 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Ehrlichia

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00022
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Glutamyl-tRNA synthetase 2 HAMAP MF_00022		PRO_0000237363
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00022
	Motif	238 – 242	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	241	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2GGL8-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: FC59CCB762FB49A0	FASTA	469	54,319
	10 20 30 40 50 60 MSHNVITRFA PSPTGHLHLG GARTALFNWL YAKHNNGKFL LRIEDTDKKR SSKELIDSII 70 80 90 100 110 120 NAMSWLKIPY DGEIVLQSKN ISRHIEIANQ LILNNKAYYC YCSEEEINKE KEEFSKKGLY 130 140 150 160 170 180 YKHNCIWKNK NFTIDNLTRV IRLRSPTEGV TSFDDKVYGN ITVSNTQLDD MVLLRSDNTP 190 200 210 220 230 240 TYLLSVVVDD HDMNITHIIR GTDHLTNTAR QLLIYNALEW NPPKFAHIPL IHDEDGNKLS 250 260 270 280 290 300 KRHQAIGIHE YKNLGILPEA ISNYLLRMGW SHEDDEIISM DQAIKWFSIK NIGQSPARLD 310 320 330 340 350 360 NKKLEFLNNH YISLTEDEVI LNMIIPIIEK KIGYMLNEVK KGYLLKGLYE LKKRTKNLVN 370 380 390 400 410 420 LANESLFYVE DVPISIDQEA SAIIKDYKHV FSILYNNLSR ISEKEWNNSI LTSTIKNISQ 430 440 450 460 NLDIKISNIY HCLRASIVGR MNAPSIIEIM INLQQEECLK RIKYAQNIE

References

[1]

"Comparative genomics of emerging human ehrlichiosis agents." Dunning Hotopp J.C., Lin M., Madupu R., Crabtree J., Angiuoli S.V., Eisen J.A., Seshadri R., Ren Q., Wu M., Utterback T.R., Smith S., Lewis M., Khouri H., Zhang C., Niu H., Lin Q., Ohashi N., Zhi N. , Nelson W.C., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sundaram J.P., Daugherty S.C., Davidsen T., Durkin A.S., Gwinn M.L., Haft D.H., Selengut J.D., Sullivan S.A., Zafar N., Zhou L., Benahmed F., Forberger H., Halpin R., Mulligan S., Robinson J., White O., Rikihisa Y., Tettelin H. PLoS Genet. 2:208-222(2006) [PubMed: 16482227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000236 Genomic DNA. Translation: ABD45013.1.
RefSeq	YP_507415.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3927900.
GenomeReviews	Gene locus ECH_0605 in contig CP000236_GR.
KEGG	ech:ECH_0605.
TIGR	ECH_0605.
Phylogenomic databases
HOGENOM	Q2GGL8.
OMA	Q2GGL8. MARYDAN.
Enzyme and pathway databases
BioCyc	ECHA205920:ECH_0605-MON.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_EHRCR

Accession

Primary (citable) accession number: Q2GGL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)