Q2GG78 (ISPE_EHRCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	ECH_0757

Organism

Ehrlichia chaffeensis (strain Arkansas) [Complete proteome] [HAMAP]

Taxonomic identifier

205920 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Ehrlichia

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000007844
Regions
	Nucleotide binding	93 – 103	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	133	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2GG78 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 0B0E3B5CA7E4340E	FASTA	282	31,277
	10 20 30 40 50 60 MLKFLVKAPA KVNLFLHITG KRSDQHHYLE SLFVFVNVYD ILEVDVGGSK RGVYFSNLRI 70 80 90 100 110 120 SKYNNTVYKA IELLLKHSAV CPNVSVSIIK NILVSAGLAG GSADAAAIMR LLGNMWNIDY 130 140 150 160 170 180 TLLQDLALKI GSDVPACLES KTLFAKGVGE DILLLPDLLL PKYIILVAPR GKTLSTAKVF 190 200 210 220 230 240 NNYQSATYSP SICDKLPVKQ DDWMELICNA KNDLLEVALK FVPEIEEILF VLKQLKNSVI 250 260 270 280 ARMTGSGATC FALFNELSHA EDAARKLQMT RPDWIIFNAK IL

References

[1]

"Comparative genomics of emerging human ehrlichiosis agents." Dunning Hotopp J.C., Lin M., Madupu R., Crabtree J., Angiuoli S.V., Eisen J.A., Seshadri R., Ren Q., Wu M., Utterback T.R., Smith S., Lewis M., Khouri H., Zhang C., Niu H., Lin Q., Ohashi N., Zhi N. , Nelson W.C., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sundaram J.P., Daugherty S.C., Davidsen T., Durkin A.S., Gwinn M.L., Haft D.H., Selengut J.D., Sullivan S.A., Zafar N., Zhou L., Benahmed F., Forberger H., Halpin R., Mulligan S., Robinson J., White O., Rikihisa Y., Tettelin H. PLoS Genet. 2:208-222(2006) [PubMed: 16482227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Arkansas.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000236 Genomic DNA. Translation: ABD44533.1.
RefSeq	YP_507555.1. NC_007799.1.
3D structure databases
ProteinModelPortal	Q2GG78.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2GG78.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3927481.
GenomeReviews	Gene locus ECH_0757 in contig CP000236_GR.
KEGG	ech:ECH_0757.
PATRIC	20576948. VBIEhrCha103583_0658.
TIGR	ECH_0757.
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	SDVPACL.
PhylomeDB	Q2GG78.
ProtClustDB	PRK14610.
Enzyme and pathway databases
BioCyc	ECHA205920:ECH_0757-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_EHRCR

Accession

Primary (citable) accession number: Q2GG78

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program