Q2GEM3 (ISPD_NEOSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	NSE_0178

Organism

Neorickettsia sennetsu (strain Miyayama) [Complete proteome] [HAMAP]

Taxonomic identifier

222891 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Neorickettsia

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_1000202893
Sites
	Site	16	1	Transition state stabilizer By similarity
	Site	24	1	Transition state stabilizer By similarity
	Site	156	1	Positions MEP for the nucleophilic attack By similarity
	Site	213	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2GEM3 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: C3794CA36EA09DE3	FASTA	232	25,310
	10 20 30 40 50 60 MGKIAGLVVA GGGGSRITNS VLPKQYLQVR GKAILQYTVE ALFAHPKIEC VHLVVNSKCE 70 80 90 100 110 120 VHYLPILRNL SGYVVSLSEA GNTRTDSVFS GLKALECLNP SHVLIQDAAR PFTTPKVINA 130 140 150 160 170 180 VIKSLLEGCE GVVPVVPVQD TIIKREPEGI VTDVNRDELR IVQTPQGFDF CKIFAAYKAH 190 200 210 220 230 FMCPSKRYTD DGSLALAHGI KVECIPGDSS NLKITHPFDL KFADFLLAQS HR

References

[1]

"Comparative genomics of emerging human ehrlichiosis agents." Dunning Hotopp J.C., Lin M., Madupu R., Crabtree J., Angiuoli S.V., Eisen J.A., Seshadri R., Ren Q., Wu M., Utterback T.R., Smith S., Lewis M., Khouri H., Zhang C., Niu H., Lin Q., Ohashi N., Zhi N. , Nelson W.C., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sundaram J.P., Daugherty S.C., Davidsen T., Durkin A.S., Gwinn M.L., Haft D.H., Selengut J.D., Sullivan S.A., Zafar N., Zhou L., Benahmed F., Forberger H., Halpin R., Mulligan S., Robinson J., White O., Rikihisa Y., Tettelin H. PLoS Genet. 2:208-222(2006) [PubMed: 16482227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Miyayama.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000237 Genomic DNA. Translation: ABD46254.1.
RefSeq	YP_506073.1. NC_007798.1.
3D structure databases
ProteinModelPortal	Q2GEM3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2GEM3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3932002.
GenomeReviews	Gene locus NSE_0178 in contig CP000237_GR.
KEGG	nse:NSE_0178.
PATRIC	22680455. VBINeoSen119815_0165.
TIGR	NSE_0178.
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	REQQDFW.
PhylomeDB	Q2GEM3.
ProtClustDB	CLSK2528037.
Enzyme and pathway databases
BioCyc	NSEN222891:NSE_0178-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_NEOSM

Accession

Primary (citable) accession number: Q2GEM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program