Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q2GE84

- LIPA_NEOSM

UniProt

Q2GE84 - LIPA_NEOSM

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Lipoyl synthase

Gene

lipA

Organism
Neorickettsia sennetsu (strain Miyayama)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi35 – 351Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi40 – 401Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi46 – 461Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi61 – 611Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi65 – 651Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi68 – 681Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciNSEN222891:GHFU-323-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:NSE_0322
OrganismiNeorickettsia sennetsu (strain Miyayama)
Taxonomic identifieri222891 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeNeorickettsia
ProteomesiUP000001942: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Lipoyl synthasePRO_1000012242Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi222891.NSE_0322.

Structurei

3D structure databases

ProteinModelPortaliQ2GE84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiFERIATN.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2GE84-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERTIEKPRI RVSADNRAFL ETSRLVSACG LNTVCQEAAC PNISECWSSK
60 70 80 90 100
HVTVMILGSV CTRACRFCNV TTGKPELLDP HEPEKLASAV GKLGLRHVVI
110 120 130 140 150
TSVDRDDLDD GGAEHFASCV RRIRETSPGT SIEVLTPDFL GKVGARDIII
160 170 180 190 200
AAAPDVFNHN VETVPRLHPK IRIKARYFNS LSLLEEVKRK DPRIFTKSGL
210 220 230 240 250
MLGLGEERSE VLQVMDDMRV AGIDFLTIGQ YLRPSKKHME VQRYATDEEF
260 270 280 290
QYYKEAAYAR GFLMVASSAL TRSSYHADED FLHLKNARAG ALAKLV
Length:296
Mass (Da):32,995
Last modified:March 21, 2006 - v1
Checksum:iDE4EAC5ABA007381
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000237 Genomic DNA. Translation: ABD45978.1.
RefSeqiWP_011451718.1. NC_007798.1.
YP_506212.1. NC_007798.1.

Genome annotation databases

EnsemblBacteriaiABD45978; ABD45978; NSE_0322.
GeneIDi3931843.
KEGGinse:NSE_0322.
PATRICi22680735. VBINeoSen119815_0300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000237 Genomic DNA. Translation: ABD45978.1 .
RefSeqi WP_011451718.1. NC_007798.1.
YP_506212.1. NC_007798.1.

3D structure databases

ProteinModelPortali Q2GE84.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 222891.NSE_0322.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD45978 ; ABD45978 ; NSE_0322 .
GeneIDi 3931843.
KEGGi nse:NSE_0322.
PATRICi 22680735. VBINeoSen119815_0300.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi FERIATN.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci NSEN222891:GHFU-323-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Miyayama.

Entry informationi

Entry nameiLIPA_NEOSM
AccessioniPrimary (citable) accession number: Q2GE84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 21, 2006
Last modified: November 26, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3