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Q2GDP4 (SYE1_NEOSM) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1
Gene names
Name:gltX1
Ordered Locus Names:NSE_0518
OrganismNeorickettsia sennetsu (strain Miyayama) [Complete proteome] [HAMAP]
Taxonomic identifier222891 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Neorickettsia

Protein attributes

Sequence length433 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B

Subunit structure

Monomer By similarity. HAMAP MF_00022_B

Subcellular location

Cytoplasm By similarity HAMAP MF_00022_B.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 433433Glutamate--tRNA ligase 1 HAMAP MF_00022_B
PRO_0000367718

Regions

Motif7 – 1711"HIGH" region HAMAP MF_00022_B
Motif230 – 2345"KMSKS" region HAMAP MF_00022_B

Sites

Binding site2331ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2GDP4 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: 862FFB1C69E5A119

FASTA43349,866
        10         20         30         40         50         60 
MITRFAPSPT GLIHLGNART ALIAYFAARS SGGEFILRID DTDVTRIKSE YVNKIFTDLS 

        70         80         90        100        110        120 
WLGIKEDLCI KQSERCELYA NAVVKLKESG RIYPCYETPE ELEIERRSLL ARALPPVYRR 

       130        140        150        160        170        180 
KNRPQHSTRL PYYRFELDPD RVVIWEDKLR GKIVIDLHST SDPIVIRENG TYTYMLPSVV 

       190        200        210        220        230        240 
DDIECRISTI IRGEDHISNT AVQIQMFEAL GCTEIPKFAH MPLLKLQTGK MSKRAGGNEI 

       250        260        270        280        290        300 
QTFRENYIEP EVICLYLLNL GSKSRSTFKN YKDYSNFNLE NYSSSSSVTV LEDEIYSLNA 

       310        320        330        340        350        360 
DFLRLSNYED LARRLGGVSK TFWDAVKNNV KSIPEVNYWW EICTTEAKLY PGIGCNAQLV 

       370        380        390        400        410        420 
RDAISVLPQE EVSRETYRRW VELIVENYRY DKREVHINLR LALTGKVGGP EMAAILPFIS 

       430 
RERILIRLND SLS

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000237 Genomic DNA. Translation: ABD46392.1.
RefSeqYP_506402.1. NC_007798.1.

3D structure databases

ProteinModelPortalQ2GDP4.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2GDP4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3931642.
GenomeReviewsGene locus NSE_0518 in contig CP000237_GR.
KEGGnse:NSE_0518.
PATRIC22681085. VBINeoSen119815_0469.
TIGRNSE_0518.

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHBG628189.
OMAARTALIC.

Enzyme and pathway databases

BioCycNSEN222891:NSE_0518-MONOMER.

Family and domain databases

HAMAPMF_00022_B. Glu_tRNA_synth_B.
[Tree]
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-synth_Ib_bac/mito.
IPR000924. Glu/Gln-tRNA-synth_Ib.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KOK01885.
PANTHERPTHR10119. Glu_tRNA-synt_1c. 1 hit.
PTHR10119:SF1. PTHR10119:SF1. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. tRNA-synt_bind. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE1_NEOSM
AccessionPrimary (citable) accession number: Q2GDP4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 21, 2006
Last modified: January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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