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Q2GDF4

- BIOB_NEOSM

UniProt

Q2GDF4 - BIOB_NEOSM

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Protein

Biotin synthase

Gene
bioB, NSE_0612
Organism
Neorickettsia sennetsu (strain Miyayama)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi55 – 551Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi58 – 581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi95 – 951Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi126 – 1261Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi186 – 1861Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi258 – 2581Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciNSEN222891:GHFU-615-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:NSE_0612
OrganismiNeorickettsia sennetsu (strain Miyayama)
Taxonomic identifieri222891 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeNeorickettsia
ProteomesiUP000001942: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325Biotin synthaseUniRule annotationPRO_0000381499Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi222891.NSE_0612.

Structurei

3D structure databases

ProteinModelPortaliQ2GDF4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2GDF4-1 [UniParc]FASTAAdd to Basket

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MAKVWQFSEA EELFHLPFLD LLYSAQHVHR ENFPHNEVQL AMLLSVKTGG    50
CPENCSYCPQ SAHYDTGLRK EPVMHVEKVL EAAKRAVELG ATRFCIGAAW 100
RGPRGKDLDI VCEMISRIKK LGLETCASLG LLSYEQAVSL KAAGLDFYNH 150
NIDTSQEYYS KIITTRRFSD RIETLENVAK AGLKICSGGI LGMGESNEDR 200
IKMLVELSHL SFPPESIPIN KLIPIPGTPL AEKNAVDPLD FVRIIALARI 250
MFPKSYVRLS AGRESMSDEL QTLCFIAGAN SIFYGEKLLT SANSDPESDE 300
KLLLKLGLLR EKSKTAVDCS INLGS 325
Length:325
Mass (Da):36,011
Last modified:July 28, 2009 - v2
Checksum:i4899075FFE3F6A33
GO

Sequence cautioni

The sequence ABD45829.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000237 Genomic DNA. Translation: ABD45829.1. Different initiation.
RefSeqiYP_506492.1. NC_007798.1.

Genome annotation databases

EnsemblBacteriaiABD45829; ABD45829; NSE_0612.
GeneIDi3931759.
KEGGinse:NSE_0612.
PATRICi22681253. VBINeoSen119815_0549.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000237 Genomic DNA. Translation: ABD45829.1 . Different initiation.
RefSeqi YP_506492.1. NC_007798.1.

3D structure databases

ProteinModelPortali Q2GDF4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 222891.NSE_0612.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD45829 ; ABD45829 ; NSE_0612 .
GeneIDi 3931759.
KEGGi nse:NSE_0612.
PATRICi 22681253. VBINeoSen119815_0549.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci NSEN222891:GHFU-615-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Miyayama.

Entry informationi

Entry nameiBIOB_NEOSM
AccessioniPrimary (citable) accession number: Q2GDF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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