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Q2GDF4 (BIOB_NEOSM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:NSE_0612
OrganismNeorickettsia sennetsu (strain Miyayama) [Complete proteome] [HAMAP]
Taxonomic identifier222891 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeNeorickettsia

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence ABD45829.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 325325Biotin synthase HAMAP-Rule MF_01694
PRO_0000381499

Sites

Metal binding511Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding551Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding951Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1261Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1861Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2581Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2GDF4 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: 4899075FFE3F6A33

FASTA32536,011
        10         20         30         40         50         60 
MAKVWQFSEA EELFHLPFLD LLYSAQHVHR ENFPHNEVQL AMLLSVKTGG CPENCSYCPQ 

        70         80         90        100        110        120 
SAHYDTGLRK EPVMHVEKVL EAAKRAVELG ATRFCIGAAW RGPRGKDLDI VCEMISRIKK 

       130        140        150        160        170        180 
LGLETCASLG LLSYEQAVSL KAAGLDFYNH NIDTSQEYYS KIITTRRFSD RIETLENVAK 

       190        200        210        220        230        240 
AGLKICSGGI LGMGESNEDR IKMLVELSHL SFPPESIPIN KLIPIPGTPL AEKNAVDPLD 

       250        260        270        280        290        300 
FVRIIALARI MFPKSYVRLS AGRESMSDEL QTLCFIAGAN SIFYGEKLLT SANSDPESDE 

       310        320 
KLLLKLGLLR EKSKTAVDCS INLGS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000237 Genomic DNA. Translation: ABD45829.1. Different initiation.
RefSeqYP_506492.1. NC_007798.1.

3D structure databases

ProteinModelPortalQ2GDF4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING222891.NSE_0612.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD45829; ABD45829; NSE_0612.
GeneID3931759.
KEGGnse:NSE_0612.
PATRIC22681253. VBINeoSen119815_0549.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OrthoDBEOG622PMP.
ProtClustDBCLSK2527769.

Enzyme and pathway databases

BioCycNSEN222891:GHFU-615-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_NEOSM
AccessionPrimary (citable) accession number: Q2GDF4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: February 19, 2014
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways