Q2GD50 (ISPE_NEOSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	NSE_0720

Organism

Neorickettsia sennetsu (strain Miyayama) [Complete proteome] [HAMAP]

Taxonomic identifier

222891 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Neorickettsia

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000071163
Regions
	Nucleotide binding	92 – 102	11	ATP Potential
Sites
	Active site	9	1	By similarity
	Active site	132	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2GD50 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 428606A703FCF9A8	FASTA	279	30,417
	10 20 30 40 50 60 MLTLRAPAKI NLFLLITGRN ESGFHLLDSV FCFTHDLYDE IIIVPTSATE NSVKFIGASQ 70 80 90 100 110 120 AIGADNSVSR VLRFVNDYAK RYFNVTLVKN LPVAAGIGGG SADAAALLRY FGGLHNISEV 130 140 150 160 170 180 LLSQVALSVG FDTLACLYSH PCHVTGMGEK IVALPESKLV YPILLVCPNF PVSSAEVYKN 190 200 210 220 230 240 FRERRSIFSA ESNLAGDHQA FEDMLSLPND LTDAAIEIAP VISDVLKSIK NCKGNKVTKL 250 260 270 CGSGPTCIGV FDTVENLRDA HSELIRHHKW WVKIAHISL

References

[1]

"Comparative genomics of emerging human ehrlichiosis agents." Dunning Hotopp J.C., Lin M., Madupu R., Crabtree J., Angiuoli S.V., Eisen J.A., Seshadri R., Ren Q., Wu M., Utterback T.R., Smith S., Lewis M., Khouri H., Zhang C., Niu H., Lin Q., Ohashi N., Zhi N. , Nelson W.C., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sundaram J.P., Daugherty S.C., Davidsen T., Durkin A.S., Gwinn M.L., Haft D.H., Selengut J.D., Sullivan S.A., Zafar N., Zhou L., Benahmed F., Forberger H., Halpin R., Mulligan S., Robinson J., White O., Rikihisa Y., Tettelin H. PLoS Genet. 2:208-222(2006) [PubMed: 16482227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Miyayama.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000237 Genomic DNA. Translation: ABD45745.1.
RefSeq	YP_506596.1. NC_007798.1.
3D structure databases
ProteinModelPortal	Q2GD50.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2GD50.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3931979.
GenomeReviews	Gene locus NSE_0720 in contig CP000237_GR.
KEGG	nse:NSE_0720.
PATRIC	22681455. VBINeoSen119815_0646.
TIGR	NSE_0720.
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	ITGRNES.
ProtClustDB	CLSK2527715.
Enzyme and pathway databases
BioCyc	NSEN222891:NSE_0720-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_NEOSM

Accession

Primary (citable) accession number: Q2GD50

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program