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Q2GCL0 (TRMB_NEOSM) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:NSE_0922
OrganismNeorickettsia sennetsu (strain Miyayama) [Complete proteome] [HAMAP]
Taxonomic identifier222891 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeNeorickettsia

Protein attributes

Sequence length217 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 217217tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288188

Sites

Active site1301 By similarity
Binding site561S-adenosyl-L-methionine By similarity
Binding site811S-adenosyl-L-methionine By similarity
Binding site1081S-adenosyl-L-methionine By similarity
Binding site1301S-adenosyl-L-methionine By similarity
Binding site1341Substrate By similarity
Binding site1661Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2GCL0 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: FCFAB0446C593638

FASTA21725,437
        10         20         30         40         50         60 
MFVHNEVNLR LEKKQFLRSY GRRRGRNFRE NKLVGLERFL ASHEKLENLK SEIVLEVGFG 

        70         80         90        100        110        120 
FGEHILKKAI DYPDKVFVGA EVYVNGIARL LEQVELHELS NVLIWNDDVR LLLEKLLCKV 

       130        140        150        160        170        180 
FHKVYILFPD PWPKRRHHKR RIVDVPFFQL LSRSLHPLAE VVVATDDHDY AQQIYQSALE 

       190        200        210 
VFSKVENTEI PKNASRFASK ASNRIFGYKM VYDATDA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000237 Genomic DNA. Translation: ABD46256.1.
RefSeqYP_506786.1. NC_007798.1.

3D structure databases

ProteinModelPortalQ2GCL0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING222891.NSE_0922.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD46256; ABD46256; NSE_0922.
GeneID3932004.
KEGGnse:NSE_0922.
PATRIC22681829. VBINeoSen119815_0824.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000154684.
KOK03439.
OMAGRNFREN.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycNSEN222891:GHFU-925-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_NEOSM
AccessionPrimary (citable) accession number: Q2GCL0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 21, 2006
Last modified: June 11, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways