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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Neorickettsia sennetsu (strain ATCC VR-367 / Miyayama) (Ehrlichia sennetsu)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56S-adenosyl-L-methionineUniRule annotation1
Binding sitei81S-adenosyl-L-methionineUniRule annotation1
Binding sitei108S-adenosyl-L-methionineUniRule annotation1
Active sitei130By similarity1
Binding sitei130S-adenosyl-L-methionineUniRule annotation1
Binding sitei134SubstrateUniRule annotation1
Binding sitei166SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:NSE_0922
OrganismiNeorickettsia sennetsu (strain ATCC VR-367 / Miyayama) (Ehrlichia sennetsu)
Taxonomic identifieri222891 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeNeorickettsia
Proteomesi
  • UP000001942 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002881881 – 217tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST217

Interactioni

Protein-protein interaction databases

STRINGi222891.NSE_0922.

Structurei

3D structure databases

ProteinModelPortaliQ2GCL0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000154684.
KOiK03439.
OMAiGFEVENS.
OrthoDBiPOG091H015P.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2GCL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFVHNEVNLR LEKKQFLRSY GRRRGRNFRE NKLVGLERFL ASHEKLENLK
60 70 80 90 100
SEIVLEVGFG FGEHILKKAI DYPDKVFVGA EVYVNGIARL LEQVELHELS
110 120 130 140 150
NVLIWNDDVR LLLEKLLCKV FHKVYILFPD PWPKRRHHKR RIVDVPFFQL
160 170 180 190 200
LSRSLHPLAE VVVATDDHDY AQQIYQSALE VFSKVENTEI PKNASRFASK
210
ASNRIFGYKM VYDATDA
Length:217
Mass (Da):25,437
Last modified:March 21, 2006 - v1
Checksum:iFCFAB0446C593638
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000237 Genomic DNA. Translation: ABD46256.1.
RefSeqiWP_011452292.1. NC_007798.1.

Genome annotation databases

EnsemblBacteriaiABD46256; ABD46256; NSE_0922.
KEGGinse:NSE_0922.
PATRICi22681829. VBINeoSen119815_0824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000237 Genomic DNA. Translation: ABD46256.1.
RefSeqiWP_011452292.1. NC_007798.1.

3D structure databases

ProteinModelPortaliQ2GCL0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi222891.NSE_0922.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD46256; ABD46256; NSE_0922.
KEGGinse:NSE_0922.
PATRICi22681829. VBINeoSen119815_0824.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000154684.
KOiK03439.
OMAiGFEVENS.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_NEOSM
AccessioniPrimary (citable) accession number: Q2GCL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 21, 2006
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.