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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Neorickettsia sennetsu (strain Miyayama)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91MagnesiumUniRule annotation
Metal bindingi70 – 701MagnesiumUniRule annotation
Metal bindingi144 – 1441MagnesiumUniRule annotation
Metal bindingi147 – 1471MagnesiumUniRule annotation

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciNSEN222891:GHFU-958-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:NSE_0955
OrganismiNeorickettsia sennetsu (strain Miyayama)
Taxonomic identifieri222891 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeNeorickettsia
ProteomesiUP000001942: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 166166Crossover junction endodeoxyribonuclease RuvCPRO_1000195266Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi222891.NSE_0955.

Structurei

3D structure databases

ProteinModelPortaliQ2GCH7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiRYDIMEA.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2GCH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYVALGVDP GLLRTGWAVV EYDGLCNVRY IDSGIVKTAS QGSLSARLEK
60 70 80 90 100
IHRGISDVIE KVNPSVAVLE KVFVNNNPYS SLNLAYCRGA LILTLALKGL
110 120 130 140 150
FIVEFAPSVL KKRITGNGRA TKAQVKYMVE QLLGLDPCLS KYSDLYDALA
160
LAASVTRYDI MEAKGT
Length:166
Mass (Da):18,019
Last modified:March 21, 2006 - v1
Checksum:i6993C039F7ECC561
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000237 Genomic DNA. Translation: ABD46339.1.
RefSeqiWP_011452325.1. NC_007798.1.
YP_506819.1. NC_007798.1.

Genome annotation databases

EnsemblBacteriaiABD46339; ABD46339; NSE_0955.
GeneIDi3931536.
KEGGinse:NSE_0955.
PATRICi22681883. VBINeoSen119815_0851.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000237 Genomic DNA. Translation: ABD46339.1.
RefSeqiWP_011452325.1. NC_007798.1.
YP_506819.1. NC_007798.1.

3D structure databases

ProteinModelPortaliQ2GCH7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi222891.NSE_0955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD46339; ABD46339; NSE_0955.
GeneIDi3931536.
KEGGinse:NSE_0955.
PATRICi22681883. VBINeoSen119815_0851.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiRYDIMEA.
OrthoDBiEOG6RG044.

Enzyme and pathway databases

BioCyciNSEN222891:GHFU-958-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Miyayama.

Entry informationi

Entry nameiRUVC_NEOSM
AccessioniPrimary (citable) accession number: Q2GCH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 21, 2006
Last modified: March 4, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.