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Q2GBM0 (TRMB_NOVAD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Saro_0305
OrganismNovosphingobium aromaticivorans (strain DSM 12444 / F199) [Complete proteome] [HAMAP]
Taxonomic identifier279238 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 238238tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288192

Regions

Region216 – 2194Substrate binding By similarity

Sites

Active site1411 By similarity
Binding site621S-adenosyl-L-methionine By similarity
Binding site871S-adenosyl-L-methionine By similarity
Binding site1191S-adenosyl-L-methionine By similarity
Binding site1411S-adenosyl-L-methionine By similarity
Binding site1451Substrate By similarity
Binding site1771Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2GBM0 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: E38CC85355B17E96

FASTA23827,165
        10         20         30         40         50         60 
MTAYKSGDPT TINRLYGRAK GKPLRQGQQA LVDELLPQIS MPAEGPITAE ALFGEPRPLH 

        70         80         90        100        110        120 
FEIGFGGGEH MAFRADMLPD HGFIGAEPFL NGVAQALTHV SGDNGQHPPI PNVRIHHGDA 

       130        140        150        160        170        180 
LEVLRRIPDG SLSFLYLLHP DPWPKARHAK RRMMNDGPVD LFAAKLRPGG EFRFGTDHAV 

       190        200        210        220        230 
YLRHALMVMR RHKHQFEWLA KDARDFQVRP GGWPETRYEH KARTVYGHEV WYFRFRRR 

« Hide

References

[1]"Complete sequence of Novosphingobium aromaticivorans DSM 12444."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. expand/collapse author list , Fredrickson J., Balkwill D., Romine M.F., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 12444 / F199.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000248 Genomic DNA. Translation: ABD24753.1.
RefSeqYP_495587.1. NC_007794.1.

3D structure databases

ProteinModelPortalQ2GBM0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING279238.Saro_0305.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD24753; ABD24753; Saro_0305.
GeneID3916242.
KEGGnar:Saro_0305.
PATRIC22783204. VBINovAro50627_0315.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMARAFYGRR.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycNARO279238:GHBU-310-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_NOVAD
AccessionPrimary (citable) accession number: Q2GBM0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 21, 2006
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways