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Q2GAJ4

- GLND_NOVAD

UniProt

Q2GAJ4 - GLND_NOVAD

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, Saro_0682
Organism
Novosphingobium aromaticivorans (strain DSM 12444 / F199)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciNARO279238:GHBU-690-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:Saro_0682
OrganismiNovosphingobium aromaticivorans (strain DSM 12444 / F199)
Taxonomic identifieri279238 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium
ProteomesiUP000009134: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 912912Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_1000078807Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi279238.Saro_0682.

Structurei

3D structure databases

ProteinModelPortaliQ2GAJ4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini486 – 591106HD
Add
BLAST
Domaini723 – 80280ACT 1
Add
BLAST
Domaini834 – 91279ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 369369UridylyltransferaseUniRule annotation
Add
BLAST
Regioni370 – 722353Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2GAJ4-1 [UniParc]FASTAAdd to Basket

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MLPRIANQRA IVDRRALADA VSAAFAEQGD RSRPAVVELL RGALDSGRAE    50
LARRLEARPS AGTECAHGQA FLVDQLVRVI HDHVVGKVYR ASNRSTGERI 100
AIIAVGGYGR GEMAPHSDVD IAFVTPIKPT SWCEQVIEAI LYFLWDLGLK 150
VGHSSRSLDE VVRMAKSDLT IRTALLEGRY VWGDRDLFDA ASARFWNEVV 200
NGTEKQFVAE KLQERNERHK RLGDSRYVVE PNVKEGKGGL RDLHTLYWIG 250
KYIHKVRDAS ELVDVGLLTA EEYRAFRRAE NFFWAVRCHL HAITRRAEDR 300
LTFDLQREVA MRMNFADRPG KSAVERFMQY FFLQAKQVGS LTGVFLAQLD 350
GQFARQKRSF FASLRSRRKK VGPFFIEGGK LGVPAEDTFQ QDPVRLVELF 400
AVAASEKVEI HPEAMRLARR DAGLIDAAVR KDQRANALFL DVLTSRNDPE 450
TVLRWMNEAG VFGRFVPDFG RVVAQMQFDM YHHYTVDEHT IRAIGLLASI 500
EKGEAKADHP LASEVVGKVA SRRVLYVATL LHDIAKGRRG DHSVLGAEVA 550
MKLCPRLGLS AAETELVAWL VRWHLLMSAT AFKRDLADYK TIADFVNTVQ 600
SQERLRLLLL LTIVDIRAVG PGVWNSWKRQ LLGDLFVSAE EVLRLGHKQH 650
GRAERIIAKK KAVGAILKER DNLIGTVGRQ LGDAYWIAEP EDIIALNLQQ 700
MDQALGELLS VEAHWYPARG ATLVTVLAAD HPGLFYRIAG GIHLAGGNII 750
DARIHTARNG TAVDNFLVQD PLGRPLNEAS QIERLKNAIA DALANRVKLV 800
PQLAARPLAR PRADAFDVRP IVIFDNKASN RFTVIEVGAR DRPALLNRLA 850
RALFEARLIV HSAHIATYGE RAVDTFYVTD VLGEKVDSEA RMKAVEKRLL 900
EAAEDRKVKD AA 912
Length:912
Mass (Da):101,972
Last modified:March 21, 2006 - v1
Checksum:iE81AD196FA1A216A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000248 Genomic DNA. Translation: ABD25129.1.
RefSeqiWP_011444343.1. NC_007794.1.
YP_495963.1. NC_007794.1.

Genome annotation databases

EnsemblBacteriaiABD25129; ABD25129; Saro_0682.
GeneIDi3918107.
KEGGinar:Saro_0682.
PATRICi22783982. VBINovAro50627_0701.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000248 Genomic DNA. Translation: ABD25129.1 .
RefSeqi WP_011444343.1. NC_007794.1.
YP_495963.1. NC_007794.1.

3D structure databases

ProteinModelPortali Q2GAJ4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 279238.Saro_0682.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD25129 ; ABD25129 ; Saro_0682 .
GeneIDi 3918107.
KEGGi nar:Saro_0682.
PATRICi 22783982. VBINovAro50627_0701.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci NARO279238:GHBU-690-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 12444 / F199.

Entry informationi

Entry nameiGLND_NOVAD
AccessioniPrimary (citable) accession number: Q2GAJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 21, 2006
Last modified: September 3, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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