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Q2GAF7 (BIOB_NOVAD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Saro_0719
OrganismNovosphingobium aromaticivorans (strain DSM 12444) [Complete proteome] [HAMAP]
Taxonomic identifier279238 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Biotin synthase HAMAP-Rule MF_01694
PRO_0000381511

Sites

Metal binding691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding761Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1131Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1441Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2041Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2821Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2GAF7 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: A6C552ABAD311F9C

FASTA35638,170
        10         20         30         40         50         60 
MQETALLERE SAGTGNAPVR TDWTREEIAA LFDLPFTELL FRAAEVHRAH HRAGEVQLCT 

        70         80         90        100        110        120 
LLSIKTGGCP EDCGYCSQSV KADSGVEATK LMEVQKVLQS AAQAKDNGSK RFCMGAAWRN 

       130        140        150        160        170        180 
PKDRDMPAII SMIKGVREMG METCMTLGML TPSQAAQLAD AGLDYYNHNI DTSPERYEEV 

       190        200        210        220        230        240 
ITTRTFADRL QTLDNVRNAG INVCSGGIVG MGETRADRVG FVHALATLEQ HPESVPVNAL 

       250        260        270        280        290        300 
VPIKGTVLGD MLADTPLAKI DDIEFVRTVA VARITMPLSM VRLSAGRESM SEATQALCFM 

       310        320        330        340        350 
AGANSIFTGD KLLTAANAGD DADAAMFKRL GLKPMEGEEP MRAMKSVGGC SGGCAA 

« Hide

References

[1]"Complete sequence of Novosphingobium aromaticivorans DSM 12444."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. expand/collapse author list , Fredrickson J., Balkwill D., Romine M.F., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 12444.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000248 Genomic DNA. Translation: ABD25166.1.
RefSeqYP_496000.1. NC_007794.1.

3D structure databases

ProteinModelPortalQ2GAF7.
SMRQ2GAF7. Positions 20-336.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING279238.Saro_0719.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD25166; ABD25166; Saro_0719.
GeneID3918543.
KEGGnar:Saro_0719.
PATRIC22784058. VBINovAro50627_0738.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2391555.

Enzyme and pathway databases

BioCycNARO279238:GHBU-728-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_NOVAD
AccessionPrimary (citable) accession number: Q2GAF7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 21, 2006
Last modified: February 19, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways