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Q2GAF7

- BIOB_NOVAD

UniProt

Q2GAF7 - BIOB_NOVAD

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Protein
Biotin synthase
Gene
bioB, Saro_0719
Organism
Novosphingobium aromaticivorans (strain DSM 12444 / F199)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi76 – 761Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi113 – 1131Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi144 – 1441Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi204 – 2041Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi282 – 2821Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciNARO279238:GHBU-728-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Saro_0719
OrganismiNovosphingobium aromaticivorans (strain DSM 12444 / F199)
Taxonomic identifieri279238 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium
ProteomesiUP000009134: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Biotin synthaseUniRule annotation
PRO_0000381511Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi279238.Saro_0719.

Structurei

3D structure databases

ProteinModelPortaliQ2GAF7.
SMRiQ2GAF7. Positions 20-336.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2GAF7-1 [UniParc]FASTAAdd to Basket

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MQETALLERE SAGTGNAPVR TDWTREEIAA LFDLPFTELL FRAAEVHRAH    50
HRAGEVQLCT LLSIKTGGCP EDCGYCSQSV KADSGVEATK LMEVQKVLQS 100
AAQAKDNGSK RFCMGAAWRN PKDRDMPAII SMIKGVREMG METCMTLGML 150
TPSQAAQLAD AGLDYYNHNI DTSPERYEEV ITTRTFADRL QTLDNVRNAG 200
INVCSGGIVG MGETRADRVG FVHALATLEQ HPESVPVNAL VPIKGTVLGD 250
MLADTPLAKI DDIEFVRTVA VARITMPLSM VRLSAGRESM SEATQALCFM 300
AGANSIFTGD KLLTAANAGD DADAAMFKRL GLKPMEGEEP MRAMKSVGGC 350
SGGCAA 356
Length:356
Mass (Da):38,170
Last modified:March 21, 2006 - v1
Checksum:iA6C552ABAD311F9C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000248 Genomic DNA. Translation: ABD25166.1.
RefSeqiYP_496000.1. NC_007794.1.

Genome annotation databases

EnsemblBacteriaiABD25166; ABD25166; Saro_0719.
GeneIDi3918543.
KEGGinar:Saro_0719.
PATRICi22784058. VBINovAro50627_0738.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000248 Genomic DNA. Translation: ABD25166.1 .
RefSeqi YP_496000.1. NC_007794.1.

3D structure databases

ProteinModelPortali Q2GAF7.
SMRi Q2GAF7. Positions 20-336.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 279238.Saro_0719.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD25166 ; ABD25166 ; Saro_0719 .
GeneIDi 3918543.
KEGGi nar:Saro_0719.
PATRICi 22784058. VBINovAro50627_0738.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci NARO279238:GHBU-728-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 12444 / F199.

Entry informationi

Entry nameiBIOB_NOVAD
AccessioniPrimary (citable) accession number: Q2GAF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 21, 2006
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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