Reviewed, UniProtKB/Swiss-Prot Q2G9R1 (SYE1_NOVAD)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Saro_0967

Organism

Novosphingobium aromaticivorans (strain DSM 12444) [Complete proteome] [HAMAP]

Taxonomic identifier

279238 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Novosphingobium

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000237380
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2G9R1-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 841180C310E17DF3	FASTA	442	48,922
	10 20 30 40 50 60 MATVTRFAPS PTGKLHVGNV RTALHNWLLA KKTGGRFLLR IDDTDAERSR EEYVESIRAD 70 80 90 100 110 120 LQWLGLIPDG EERQSLRTEL YEREFQRLVE AGRIYRAYET AQELDLKRKI LLGRGLPPIY 130 140 150 160 170 180 DRAALKLTEA DHAAKAAAGE RPHWRFLLDH DQPITWDDGI RGPQSFDPRQ MSDPVIRRAD 190 200 210 220 230 240 GSWLYMLPSA IDDIAMGITD VLRGEDHVSN TATQIQMFTA LGAEPPRFAH EALLTGSEGK 250 260 270 280 290 300 LSKRLGALGM ADFREQGIEP EAIVALLARL GTSDPVDAAL DAAALATSFD LSRFGRAPAR 310 320 330 340 350 360 FDEADLHRVN AQIVHRLPYA RVAHLLPQGM GEAAWEAIRP NLAHIDEARD WWNVVTGPVT 370 380 390 400 410 420 APTFDDETRA FLAQAAKTAA SLDWSADPWR ALTATLKDGT GRKGKALFLP LRQALTGHDH 430 440 GPEMAALLPL IAQDEAVRRL SA

References

[1]

"Complete sequence of Novosphingobium aromaticivorans DSM 12444." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. , Fredrickson J., Balkwill D., Romine M.F., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000248 Genomic DNA. Translation: ABD25412.1.
RefSeq	YP_496246.1.
3D structure databases
SMR	Q2G9R1. Positions 3-441.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2G9R1.
Genome annotation databases
GeneID	3915749.
GenomeReviews	Gene locus Saro_0967 in contig CP000248_GR.
KEGG	nar:Saro_0967.
NMPDR	fig|48935.1.peg.3073.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	LRLDDTD.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_NOVAD

Accession

Primary (citable) accession number: Q2G9R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 21, 2006
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)