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Q2G992 (DDL_NOVAD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Saro_1136
OrganismNovosphingobium aromaticivorans (strain DSM 12444) [Complete proteome] [HAMAP]
Taxonomic identifier279238 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341141

Regions

Domain104 – 304201ATP-grasp
Nucleotide binding130 – 18758ATP By similarity

Sites

Metal binding2551Magnesium or manganese 1 By similarity
Metal binding2711Magnesium or manganese 1 By similarity
Metal binding2711Magnesium or manganese 2 By similarity
Metal binding2731Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2G992 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: 6F097372C9B9BBCA

FASTA31333,694
        10         20         30         40         50         60 
MSLPKLHVAV LMGGWSSERP VSLMSGEGVA KALESKGHQV TRIDMDRDVA LRLAEAKPDV 

        70         80         90        100        110        120 
VFNALHGTPG EDGSIQGLMD IMGLTYTHSG LVTSVIAIDK ELTKQALVPH GIPMPGGRMV 

       130        140        150        160        170        180 
KTADLYKADP LPRPYVLKPV NEGSSVGVAI VTAEGNYGSP ISAASKGPWQ EFDQLLAEPF 

       190        200        210        220        230        240 
IRGRELTTAV IGDRALLVTE LRPKSGFYDF DAKYTEGMTD HICPAEIPDE ITEACKDIAL 

       250        260        270        280        290        300 
RAHRLLGCKG TSRSDFRWDD EQGVEGLFLL EVNTQPGMTP LSLVPEQARA LGMDYSDLVE 

       310 
AIIAEALKDA GKA 

« Hide

References

[1]"Complete sequence of Novosphingobium aromaticivorans DSM 12444."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. expand/collapse author list , Fredrickson J., Balkwill D., Romine M.F., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 12444.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000248 Genomic DNA. Translation: ABD25581.1.
RefSeqYP_496415.1. NC_007794.1.

3D structure databases

ProteinModelPortalQ2G992.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING279238.Saro_1136.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD25581; ABD25581; Saro_1136.
GeneID3916432.
KEGGnar:Saro_1136.
PATRIC22784906. VBINovAro50627_1158.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAWREAARY.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycNARO279238:GHBU-1150-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_NOVAD
AccessionPrimary (citable) accession number: Q2G992
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 21, 2006
Last modified: February 19, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways