Q2G8Y3 (EFG_NOVAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	Saro_1246

Organism

Novosphingobium aromaticivorans (strain DSM 12444) [Complete proteome] [HAMAP]

Taxonomic identifier

279238 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Novosphingobium

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 690	690	Elongation factor G HAMAP MF_00054_B		PRO_0000263479
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	135 – 138	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2G8Y3 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: D79E17270D9B82AE	FASTA	690	76,181
	10 20 30 40 50 60 MARSHPLERY RNFGIMAHID AGKTTTTERI LYYTGKSYKI GEVHEGAATM DWMEQEQERG 70 80 90 100 110 120 ITITSAATTC FWNDHRLNII DTPGHVDFTI EVERSLRVLD GAVAAFDGVA GVEPQSETVW 130 140 150 160 170 180 RQADKYGVPR MCYINKLDRT GANFYYCVQT IIDRLGAKPA VLYLPIGAES EFKGLVDLIN 190 200 210 220 230 240 ERAIIWKDES LGAEFFYEDI PADMADKAAE YREKLIELAV EQDDAAMEAY LEGTMPDAAT 250 260 270 280 290 300 LKALLRKGTL AHAFVPVLCG SSFKNKGVQA LLDAVVDFMP SPLDIEDVQG INPDTDEPDS 310 320 330 340 350 360 RATSDDAPFS ALAFKIMNDP FVGSLTFTRI YSGTLSKGSY LNSVKNKKEK VGRMLLMHAN 370 380 390 400 410 420 SREDIEEAYA GDIVALAGLK ETTTGDTLCS EKQPIILERM EFPEPVIELS VEPKTKADQE 430 440 450 460 470 480 KMGIALNRLA AEDPSFRVST DHESGQTIIK GMGELHLEIL VDRMKREFKV EANVGAPQVA 490 500 510 520 530 540 YREYLAKAID LDHTHKKQSG GTGQFGRVKV KVTPGERGSG FVFKDEIKGG NIPKEYIPAI 550 560 570 580 590 600 EKGFRETAAT GSLIGFPIID FEVLLYDGAY HDVDSSALAF EICARGAMRE AAQKAGIKLL 610 620 630 640 650 660 EPIMKVEVIT PDEYLGDVIG DINSRRGQIQ GTDTRGNAQA VTAMVPLANM FGYVNQLRSF 670 680 690 TQGRANYSMF FDHYDEVPAN VATELKAKLA

References

[1]

"Complete sequence of Novosphingobium aromaticivorans DSM 12444." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. , Fredrickson J., Balkwill D., Romine M.F., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 12444.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000248 Genomic DNA. Translation: ABD25690.1.
RefSeq	YP_496524.1. NC_007794.1.
3D structure databases
ProteinModelPortal	Q2G8Y3.
SMR	Q2G8Y3. Positions 4-685.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2G8Y3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3917877.
GenomeReviews	Gene locus Saro_1246 in contig CP000248_GR.
KEGG	nar:Saro_1246.
NMPDR	fig|48935.1.peg.1784.
PATRIC	22785140. VBINovAro50627_1273.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0480.
HOGENOM	HBG737692.
OMA	CNEWREK.
PhylomeDB	Q2G8Y3.
ProtClustDB	PRK00007.
Enzyme and pathway databases
BioCyc	NARO279238:SARO_1246-MONOMER.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_NOVAD

Accession

Primary (citable) accession number: Q2G8Y3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program