Q2G8Q8 (Q2G8Q8_NOVAD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase HAMAP MF_01374
EC=3.1.2.6 HAMAP MF_01374

Alternative name(s):
Glyoxalase II HAMAP MF_01374

Gene names

Name:	gloB HAMAP MF_01374
Ordered Locus Names:	Saro_1321

Organism

Novosphingobium aromaticivorans (strain DSM 12444) [Complete proteome] [HAMAP]

Taxonomic identifier

279238 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Novosphingobium

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374 SAAS SAAS017782
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374 SAAS SAAS017782
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374 SAAS SAAS017782
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374 SAAS SAAS017782
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. HAMAP MF_01374

Ontologies

Keywords
Ligand	Metal-binding HAMAP MF_01374 SAAS SAAS017782 Zinc HAMAP MF_01374 SAAS SAAS017782
Molecular function	Hydrolase HAMAP MF_01374 SAAS SAAS017782 EMBL ABD25765.1
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	56	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	58	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	60	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	61	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	114	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	133	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	133	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	171	1	Zinc 2 By similarity HAMAP MF_01374

Sequences

	Sequence		Length	Mass (Da)
	Q2G8Q8 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: DB71F3D77FC7B381	FASTA	254	27,179
	10 20 30 40 50 60 MTLEIHQFPC LSDNYGFLVH DSDSGETACI DTPDAEAYLR EANARGWRIT QIWNTHWHPD 70 80 90 100 110 120 HAGGNEAIKA ATGCTVVAPE DDAGKIAGVD RAVAHGDTVQ LGSFTAEVID VGGHTMGHCA 130 140 150 160 170 180 YHLPEASVAF VGDALFPLGC GRMFEGTPPQ FWASLSRIKA LPPGTTLYSA HEYTQSNARF 190 200 210 220 230 240 ALHADPDNAA LRAYADGVEA RRARGEWTVP TTLAAELAAN PFLRADTPGM AARWGGSSPV 250 DTFAALRAAK DTFR

References

[1]

"Complete sequence of Novosphingobium aromaticivorans DSM 12444." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. , Fredrickson J., Balkwill D., Romine M.F., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 12444.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000248 Genomic DNA. Translation: ABD25765.1.
RefSeq	YP_496599.1. NC_007794.1.
3D structure databases
ProteinModelPortal	Q2G8Q8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2G8Q8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3917770.
GenomeReviews	Gene locus Saro_1321 in contig CP000248_GR.
KEGG	nar:Saro_1321.
NMPDR	fig|48935.1.peg.1902.
PATRIC	22785300. VBINovAro50627_1352.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	IGCGRVF.
PhylomeDB	Q2G8Q8.
ProtClustDB	CLSK753563.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q2G8Q8_NOVAD

Accession

Primary (citable) accession number: Q2G8Q8

Entry history

Integrated into UniProtKB/TrEMBL:	March 21, 2006
Last sequence update:	March 21, 2006
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)