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Q2G8D6 (DAPA_NOVAD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:Saro_1443
OrganismNovosphingobium aromaticivorans (strain DSM 12444) [Complete proteome] [HAMAP]
Taxonomic identifier279238 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Novosphingobium

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 296296Dihydrodipicolinate synthase HAMAP MF_00418
PRO_1000050234

Regions

Region47 – 482Pyruvate binding By similarity

Sites

Active site1621Schiff-base intermediate with substrate By similarity
Binding site1051Pyruvate By similarity
Site1321Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2G8D6 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: 2AEAF4E1332AE740

FASTA29631,695
        10         20         30         40         50         60 
MFSGSIPALV TPFRDGAFDE KAFRRLVDWQ IENGSSALVP CGTTGEASTL SNAEHHRVIE 

        70         80         90        100        110        120 
VCVEQAAGRV PVIAGCGSND TMNALLHMNF SKKCGAQAAL CVAPYYNRPS QAGIIAHFSY 

       130        140        150        160        170        180 
LAEHNDLPIV LYNVPGRTVT DILPETVCEL AKRYPDKIIG IKDASGDLSR VTDHRMGIGK 

       190        200        210        220        230        240 
HFCQLSGDDE LALPANAAGA VGCISVTANV APRLCADFQA ACAANDLEKA RELNDKLYPL 

       250        260        270        280        290 
HYAMFEDASP GPVKYALSRV FADINEDLRL PMVPCNEAAR KAVDAALVHA GLLELA

« Hide

References

[1]"Complete sequence of Novosphingobium aromaticivorans DSM 12444."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. expand/collapse author list , Fredrickson J., Balkwill D., Romine M.F., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 12444.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000248 Genomic DNA. Translation: ABD25887.1.
RefSeqYP_496721.1. NC_007794.1.

3D structure databases

HSSPHSSP built from PDB template 1O5K based on UniProtKB Q9X1K9.
ProteinModelPortalQ2G8D6.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2G8D6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3916107.
GenomeReviewsGene locus Saro_1443 in contig CP000248_GR.
KEGGnar:Saro_1443.
NMPDRfig|48935.1.peg.2227.
PATRIC22785562. VBINovAro50627_1478.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0329.
HOGENOMHBG358848.
OMAELATHKN.
PhylomeDBQ2G8D6.
ProtClustDBPRK03170.

Enzyme and pathway databases

BioCycNARO279238:SARO_1443-MONOMER.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_NOVAD
AccessionPrimary (citable) accession number: Q2G8D6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 21, 2006
Last modified: January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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