Q2G7D0 (Q2G7D0_NOVAD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Saro_1803

Organism

Novosphingobium aromaticivorans (strain DSM 12444) [Complete proteome] [HAMAP]

Taxonomic identifier

279238 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Novosphingobium

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q2G7D0 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: E8CDEAC923AD4021	FASTA	859	94,437
	10 20 30 40 50 60 MQLVIVESPA KAKTIEKYLG PGYKVLASYG HVRDLPVKDG SVRPDEDFAM DWELYGDKQA 70 80 90 100 110 120 RVKAISDAAK GADRLILATD PDREGEAISW HVRELLAKRR VLPKEVERVT FNAITKQTVT 130 140 150 160 170 180 DAMKKPRALD QDLIDAYLAR RALDYLFGFT LSPVLWRKLP GAKSAGRVQS VALRLIVERE 190 200 210 220 230 240 REIEAFRAQE YWSVIARLEH AGTEFAARLV KFDGQKLDKL TLGDEGAAMR AKGVVEAATF 250 260 270 280 290 300 RVEEVETKPT RRNPYPPFTT STLQQEAARK LGFSASHTMR VAQTLYEAGA ITYMRTDGVQ 310 320 330 340 350 360 MDPSAISAAR KAISDRYDGH YLPEKPRHYE TKAKNAQEAH EAIRPTDFSK DRYGSADEAR 370 380 390 400 410 420 LYDLIYKRAM ASQMASANIE RTTVTLRDGT GKHELRATGQ VVKFPGFLAV YEEGRDQKQD 430 440 450 460 470 480 GDEEDGSLLP AMASGDTPAK RGVDATQHFT QPPPRYSEAS LVKRLEELGI GRPSTYAATL 490 500 510 520 530 540 QVLKDRNYVR TEKNRFFAEE SGRLLTAFLE RFFPRYVAYE FTAGMEEELD DVSGGRAEWK 550 560 570 580 590 600 KVLADFWRDF KPKSDEVMEK KPSEVTAELD EFLSDYLFPP RADGTDPRLC PKCEEGRLSL 610 620 630 640 650 660 RGGRYGAFVA CSNYPECKYT RKFAQPGGAD GDGGEDGALG KDPETGLEVV RKAGRFGPYI 670 680 690 700 710 720 QLGDGKEAKR ASIPKDIGEL DLEWALKLLS LPREVGVHPE TGNPITASIG RYGPYLAHDG 730 740 750 760 770 780 KYARLKSTAE VFETGMNAAV MLLAAAAAGA GARGSRAAAE PLKVFGAHPT SGGEIKLMAG 790 800 810 820 830 840 RYGPYVTDGT TNATLPRDKQ PEALTLEEAI TLIDERAAKG PAKGKKKAPA KKASAAKKPP 850 AAKKAPAKKA APKKAAAAE

References

[1]

"Complete sequence of Novosphingobium aromaticivorans DSM 12444." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. , Fredrickson J., Balkwill D., Romine M.F., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 12444.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000248 Genomic DNA. Translation: ABD26243.1.
RefSeq	YP_497077.1. NC_007794.1.
3D structure databases
ProteinModelPortal	Q2G7D0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2G7D0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3918362.
GenomeReviews	Gene locus Saro_1803 in contig CP000248_GR.
KEGG	nar:Saro_1803.
PATRIC	22786294. VBINovAro50627_1842.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	KKVMPRL.
PhylomeDB	Q2G7D0.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q2G7D0_NOVAD

Accession

Primary (citable) accession number: Q2G7D0

Entry history

Integrated into UniProtKB/TrEMBL:	March 21, 2006
Last sequence update:	March 21, 2006
Last modified:	December 14, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)