Reviewed, UniProtKB/Swiss-Prot Q2G781 (GCSPA_NOVAD)

Names and origin

Protein names

Recommended name:
    Probable glycine dehydrogenase [decarboxylating] subunit 1
    EC=1.4.4.2
Alternative name(s):
    Glycine decarboxylase subunit 1
    Glycine cleavage system P-protein subunit 1

Gene names

Name:	gcvPA
Ordered Locus Names:	Saro_1852

Organism

Novosphingobium aromaticivorans (strain DSM 12444) [Complete proteome] [HAMAP]

Taxonomic identifier

279238 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Novosphingobium

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity.
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. HAMAP MF_00712
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is an heterodimer of two subunits By similarity.
Sequence similarities	Belongs to the gcvP family. N-terminal subunit subfamily.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Probable glycine dehydrogenase [decarboxylating] subunit 1 HAMAP MF_00712		PRO_1000045672

Sequences

	Sequence		Length	Mass (Da)
	Q2G781-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: BCD17DC65E74DCFA	FASTA	452	47,592
	10 20 30 40 50 60 MRYLPLTDAD RSAMLSVVGA GSVDELFADV PAEARLSAPI AGLPNHASEM AVERHMARLS 70 80 90 100 110 120 ANNVTAGSVP FFLGAGAYRH HVPATVDHMI QRGEFLTAYT PYQPEIAQGT LQVLFEFQTQ 130 140 150 160 170 180 VARLFGTDVA NASLYDGSTA CWEAIAMAGR ITKRGKALLS GGLHPHYVET ARTMARFTGD 190 200 210 220 230 240 VLDTSAPVLT AAPDDDALVA RIDGETSCVV VQYPDILGRI PDLAKIAAAA QAQGALLITV 250 260 270 280 290 300 VTEPVALGVL QSPGSLGADI VVGEGQSLGV GLQFGGPYLG LFGCREKYLR QIPGRLCGET 310 320 330 340 350 360 VDADGKRGFV LTLSTREQHI RREKATSNIC TNSGLCALAF SIHLTLLGGS GLADMARLSH 370 380 390 400 410 420 LAARKTAAAL AQVSGIEVVN SHFFNEFTVA LPHDARQIVR DLADRHVLGG VSLGRLYPQE 430 440 450 AALANGMVVA ATECTTDEDI AALVAALKEV LA

References

[1]

"Complete sequence of Novosphingobium aromaticivorans DSM 12444." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. , Fredrickson J., Balkwill D., Romine M.F., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000248 Genomic DNA. Translation: ABD26292.1.
RefSeq	YP_497126.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3918414.
GenomeReviews	Gene locus Saro_1852 in contig CP000248_GR.
KEGG	nar:Saro_1852.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2G781.
OMA	Q2G781. VANASMY.
Enzyme and pathway databases
BioCyc	NARO279238:SARO_1852-MON.
Family and domain databases
HAMAP	MF_00712. [Tree]
InterPro	IPR003437. GDC-P. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11773. GDC-P. 1 hit.
Pfam	PF02347. GDC-P. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCSPA_NOVAD

Accession

Primary (citable) accession number: Q2G781

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)