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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Novosphingobium aromaticivorans (strain DSM 12444 / F199)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciNARO279238:GHBU-1945-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:Saro_1915
OrganismiNovosphingobium aromaticivorans (strain DSM 12444 / F199)
Taxonomic identifieri279238 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium
ProteomesiUP000009134 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000346369Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi279238.Saro_1915.

Structurei

3D structure databases

ProteinModelPortaliQ2G718.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1206.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2G718-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHQVHIIGG GMAGTEAAWQ LARRGIRVRL SEMRGGGDTT PAHNGDGLAE
60 70 80 90 100
LVCSNSFRSD DDEKNAVGLL HYEMRQCDSL LMAAAAKARV PAGSALAVDR
110 120 130 140 150
DVFSAEVEAA LRAQPTLEIV RERVDVLPSD GLTIVATGPL TAPSLANSIG
160 170 180 190 200
SATGADSLAF FDAIAPIVYR DSIDMGVAWM ASRWDKGAEA SLAMGGDGRD
210 220 230 240 250
YINCPMTRDQ YLAFREELLA GEKTEFKEWE ANTPYFDGCM PIEVMAARGE
260 270 280 290 300
ETLRFGPMKP VGLDNPHWAT AEHPNGRWPY AVVQLRQDNK LGTLWNMVGF
310 320 330 340 350
QTKLKHAEQV RVFRTIPGLE NAEFARLGGL HRNTFLNSPT LLDRQLRLKS
360 370 380 390 400
APNVRFAGQI TGCEGYVESG SVGMLAGLMV AAQIAGLDWS PPPRTTALGA
410 420 430 440 450
LLAHITGDAE AESFQPMNVN FGLFSPVDAS VKKKVRKEAY TARAKADLSD

WIATLA
Length:456
Mass (Da):49,316
Last modified:March 21, 2006 - v1
Checksum:i22DA550E6F24509F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000248 Genomic DNA. Translation: ABD26355.1.
RefSeqiWP_011445565.1. NC_007794.1.

Genome annotation databases

EnsemblBacteriaiABD26355; ABD26355; Saro_1915.
KEGGinar:Saro_1915.
PATRICi22786536. VBINovAro50627_1961.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000248 Genomic DNA. Translation: ABD26355.1.
RefSeqiWP_011445565.1. NC_007794.1.

3D structure databases

ProteinModelPortaliQ2G718.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279238.Saro_1915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD26355; ABD26355; Saro_1915.
KEGGinar:Saro_1915.
PATRICi22786536. VBINovAro50627_1961.

Phylogenomic databases

eggNOGiCOG1206.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Enzyme and pathway databases

BioCyciNARO279238:GHBU-1945-MONOMER.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 12444 / F199.

Entry informationi

Entry nameiTRMFO_NOVAD
AccessioniPrimary (citable) accession number: Q2G718
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 21, 2006
Last modified: July 22, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.