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Q2G718

- TRMFO_NOVAD

UniProt

Q2G718 - TRMFO_NOVAD

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Protein
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
Gene
trmFO, Saro_1915
Organism
Novosphingobium aromaticivorans (strain DSM 12444 / F199)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FAD By similarity.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FAD By similarity

GO - Molecular functioni

  1. 5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity Source: UniProtKB-HAMAP
  2. flavin adenine dinucleotide binding Source: UniProtKB-HAMAP
  3. methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciNARO279238:GHBU-1945-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO (EC:2.1.1.74)
    Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase
    Gene namesi
    Name:trmFO
    Ordered Locus Names:Saro_1915
    OrganismiNovosphingobium aromaticivorans (strain DSM 12444 / F199)
    Taxonomic identifieri279238 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium
    ProteomesiUP000009134: Chromosome

    Subcellular locationi

    Cytoplasm By similarity UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 456456Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation
    PRO_0000346369Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi279238.Saro_1915.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2G718.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MnmG family. TrmFO subfamily.

    Phylogenomic databases

    eggNOGiCOG1206.
    HOGENOMiHOG000252054.
    KOiK04094.
    OMAiEIYFEGC.
    OrthoDBiEOG6J74VT.

    Family and domain databases

    HAMAPiMF_01037. TrmFO.
    InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
    IPR002218. GIDA-rel.
    IPR020595. GIDA-rel_CS.
    [Graphical view]
    PfamiPF01134. GIDA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
    PROSITEiPS01281. GIDA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2G718-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTHQVHIIGG GMAGTEAAWQ LARRGIRVRL SEMRGGGDTT PAHNGDGLAE    50
    LVCSNSFRSD DDEKNAVGLL HYEMRQCDSL LMAAAAKARV PAGSALAVDR 100
    DVFSAEVEAA LRAQPTLEIV RERVDVLPSD GLTIVATGPL TAPSLANSIG 150
    SATGADSLAF FDAIAPIVYR DSIDMGVAWM ASRWDKGAEA SLAMGGDGRD 200
    YINCPMTRDQ YLAFREELLA GEKTEFKEWE ANTPYFDGCM PIEVMAARGE 250
    ETLRFGPMKP VGLDNPHWAT AEHPNGRWPY AVVQLRQDNK LGTLWNMVGF 300
    QTKLKHAEQV RVFRTIPGLE NAEFARLGGL HRNTFLNSPT LLDRQLRLKS 350
    APNVRFAGQI TGCEGYVESG SVGMLAGLMV AAQIAGLDWS PPPRTTALGA 400
    LLAHITGDAE AESFQPMNVN FGLFSPVDAS VKKKVRKEAY TARAKADLSD 450
    WIATLA 456
    Length:456
    Mass (Da):49,316
    Last modified:March 21, 2006 - v1
    Checksum:i22DA550E6F24509F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000248 Genomic DNA. Translation: ABD26355.1.
    RefSeqiWP_011445565.1. NC_007794.1.
    YP_497189.1. NC_007794.1.

    Genome annotation databases

    EnsemblBacteriaiABD26355; ABD26355; Saro_1915.
    GeneIDi3917138.
    KEGGinar:Saro_1915.
    PATRICi22786536. VBINovAro50627_1961.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000248 Genomic DNA. Translation: ABD26355.1 .
    RefSeqi WP_011445565.1. NC_007794.1.
    YP_497189.1. NC_007794.1.

    3D structure databases

    ProteinModelPortali Q2G718.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 279238.Saro_1915.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD26355 ; ABD26355 ; Saro_1915 .
    GeneIDi 3917138.
    KEGGi nar:Saro_1915.
    PATRICi 22786536. VBINovAro50627_1961.

    Phylogenomic databases

    eggNOGi COG1206.
    HOGENOMi HOG000252054.
    KOi K04094.
    OMAi EIYFEGC.
    OrthoDBi EOG6J74VT.

    Enzyme and pathway databases

    BioCyci NARO279238:GHBU-1945-MONOMER.

    Family and domain databases

    HAMAPi MF_01037. TrmFO.
    InterProi IPR004417. Folate-dep_Ribothymidyl_synth.
    IPR002218. GIDA-rel.
    IPR020595. GIDA-rel_CS.
    [Graphical view ]
    Pfami PF01134. GIDA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00137. gid_trmFO. 1 hit.
    PROSITEi PS01281. GIDA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 12444 / F199.

    Entry informationi

    Entry nameiTRMFO_NOVAD
    AccessioniPrimary (citable) accession number: Q2G718
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: March 21, 2006
    Last modified: September 3, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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