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Q2G718 (TRMFO_NOVAD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene names
Name:trmFO
Ordered Locus Names:Saro_1915
OrganismNovosphingobium aromaticivorans (strain DSM 12444 / F199) [Complete proteome] [HAMAP]
Taxonomic identifier279238 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium

Protein attributes

Sequence length456 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP-Rule MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + uracil54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil54 in tRNA + FAD. HAMAP-Rule MF_01037

Cofactor

FAD By similarity. HAMAP-Rule MF_01037

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01037.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 456456Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP-Rule MF_01037
PRO_0000346369

Regions

Nucleotide binding9 – 146FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2G718 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: 22DA550E6F24509F

FASTA45649,316
        10         20         30         40         50         60 
MTHQVHIIGG GMAGTEAAWQ LARRGIRVRL SEMRGGGDTT PAHNGDGLAE LVCSNSFRSD 

        70         80         90        100        110        120 
DDEKNAVGLL HYEMRQCDSL LMAAAAKARV PAGSALAVDR DVFSAEVEAA LRAQPTLEIV 

       130        140        150        160        170        180 
RERVDVLPSD GLTIVATGPL TAPSLANSIG SATGADSLAF FDAIAPIVYR DSIDMGVAWM 

       190        200        210        220        230        240 
ASRWDKGAEA SLAMGGDGRD YINCPMTRDQ YLAFREELLA GEKTEFKEWE ANTPYFDGCM 

       250        260        270        280        290        300 
PIEVMAARGE ETLRFGPMKP VGLDNPHWAT AEHPNGRWPY AVVQLRQDNK LGTLWNMVGF 

       310        320        330        340        350        360 
QTKLKHAEQV RVFRTIPGLE NAEFARLGGL HRNTFLNSPT LLDRQLRLKS APNVRFAGQI 

       370        380        390        400        410        420 
TGCEGYVESG SVGMLAGLMV AAQIAGLDWS PPPRTTALGA LLAHITGDAE AESFQPMNVN 

       430        440        450 
FGLFSPVDAS VKKKVRKEAY TARAKADLSD WIATLA 

« Hide

References

[1]"Complete sequence of Novosphingobium aromaticivorans DSM 12444."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. expand/collapse author list , Fredrickson J., Balkwill D., Romine M.F., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 12444 / F199.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000248 Genomic DNA. Translation: ABD26355.1.
RefSeqYP_497189.1. NC_007794.1.

3D structure databases

ProteinModelPortalQ2G718.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING279238.Saro_1915.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD26355; ABD26355; Saro_1915.
GeneID3917138.
KEGGnar:Saro_1915.
PATRIC22786536. VBINovAro50627_1961.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1206.
HOGENOMHOG000252054.
KOK04094.
OMAEIYFEGC.
OrthoDBEOG6J74VT.

Enzyme and pathway databases

BioCycNARO279238:GHBU-1945-MONOMER.

Family and domain databases

HAMAPMF_01037. TrmFO.
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00137. gid_trmFO. 1 hit.
PROSITEPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMFO_NOVAD
AccessionPrimary (citable) accession number: Q2G718
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 21, 2006
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families