Reviewed, UniProtKB/Swiss-Prot Q2G6Q3 (SYE2_NOVAD)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 2
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 2
      Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	Saro_2031

Organism

Novosphingobium aromaticivorans (strain DSM 12444) [Complete proteome] [HAMAP]

Taxonomic identifier

279238 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Novosphingobium

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00022
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Glutamyl-tRNA synthetase 2 HAMAP MF_00022		PRO_0000237381
Regions
	Motif	24 – 34	11	"HIGH" region HAMAP MF_00022
	Motif	258 – 262	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	261	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2G6Q3-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 8DCC1B1CDBC397A2	FASTA	487	53,204
	10 20 30 40 50 60 MASATDTVSA GSTPRDKVVT RFAPSPTGFL HIGGARTALF NWLYARHHGG TYLLRIEDTD 70 80 90 100 110 120 RARSTDAAID AIFDGLEWLG LGGDEPAVFQ FARSDRHAEV AHKLLEAGHA YRCYLTQEEL 130 140 150 160 170 180 AARRELAQAE RRPFRIDSEW RDATPDQWPA DQSYVVRMKA PREGETTIVD KVQGSITVQN 190 200 210 220 230 240 SELDDFIILR SDGTPTYMLA VVVDDHDMGV THVIRGDDHI NNAFRQLVII RGMHAIEGGW 250 260 270 280 290 300 PDPVYAHIPL IHGADGAKLS KRHGALGVDA YRDEMGLLPE AVFNYLLRLG WGHGDEEIIS 310 320 330 340 350 360 REQAVAWFDI GDVNKGASRF DLKKLLNLNG HYIREADDAR LAALVAPRLA TLAPGFAPDK 370 380 390 400 410 420 GLDLLTRAMP VLKVRAADIN ELAAGSVFLF AQRPLAMAEK AASLLTDDAR AILTKVAGVL 430 440 450 460 470 480 EAENVWTTGV LEATVKQMAE ELGIGLGKIA QPLRASLTGQ TTSPGIFDVL ALLGKDESLS RIRDQAA

References

[1]

"Complete sequence of Novosphingobium aromaticivorans DSM 12444." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. , Fredrickson J., Balkwill D., Romine M.F., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000248 Genomic DNA. Translation: ABD26470.1.
RefSeq	YP_497304.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3917678.
GenomeReviews	Gene locus Saro_2031 in contig CP000248_GR.
KEGG	nar:Saro_2031.
NMPDR	fig|48935.1.peg.2297.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2G6Q3.
OMA	Q2G6Q3. MARYDAN.
Enzyme and pathway databases
BioCyc	NARO279238:SARO_2031-MON.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_NOVAD

Accession

Primary (citable) accession number: Q2G6Q3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)