Q2G5F0 (KITH_NOVAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	Saro_2487

Organism

Novosphingobium aromaticivorans (strain DSM 12444) [Complete proteome] [HAMAP]

Taxonomic identifier

279238 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Novosphingobium › Sphingomonas aromaticivorans

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	Thymidine kinase HAMAP-Rule MF_00124		PRO_0000242798
Regions
	Nucleotide binding	9 – 16	8	ATP By similarity
	Nucleotide binding	87 – 90	4	ATP By similarity
Sites
	Active site	88	1	Proton acceptor Potential
	Metal binding	145	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity
	Metal binding	182	1	Zinc By similarity
	Metal binding	185	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2G5F0 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: F300431FA486C2DF	FASTA	192	21,079
	10 20 30 40 50 60 MAKLYFYYAS MNAGKSTNLL QADFNYRERG MATMLWTAAL DDRGGERAIE SRIGLGADAH 70 80 90 100 110 120 RFDAGTDLWQ RISAAHAVQP LSCVLVDEAQ FLRRDQVWQL ARVADAAGIP VLCYGLRTDF 130 140 150 160 170 180 QGELFEGSAA LLGIADSLIE LKAVCHCGRK ATMNLRVDDS GAAVRAGRQT EIGGNDRYVA 190 LCRRHFSEAM GQ

References

[1]

"Complete sequence of Novosphingobium aromaticivorans DSM 12444." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. , Fredrickson J., Balkwill D., Romine M.F., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 12444.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000248 Genomic DNA. Translation: ABD26923.1.
RefSeq	YP_497757.1. NC_007794.1.
3D structure databases
ProteinModelPortal	Q2G5F0.
ModBase	Search...
Protein-protein interaction databases
STRING	279238.Saro_2487.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD26923; ABD26923; Saro_2487.
GeneID	3916807.
KEGG	nar:Saro_2487.
PATRIC	22787726. VBINovAro50627_2543.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076391.
KO	K00857.
OMA	FRYGAMN.
ProtClustDB	PRK04296.
Enzyme and pathway databases
BioCyc	NARO279238:GHBU-3179-MONOMER.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_NOVAD

Accession

Primary (citable) accession number: Q2G5F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 21, 2006
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program