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Q2G506

- ATM1_NOVAD

UniProt

Q2G506 - ATM1_NOVAD

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Protein

ATM1-type heavy metal exporter

Gene
atm1, Saro_2631
Organism
Novosphingobium aromaticivorans (strain DSM 12444 / F199)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates the ATP-dependent export of glutathione-conjugated substrates, such as heavy metal-glutathione conjugates. ATP hydrolysis is stimulated by glutathione binding. Protects cells against toxic heavy metal ions, such as silver and mercury ions. May also mediate the transport of glutathione-conjugated aromatic hydrocarbons, such as dinitrobenzene.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei319 – 3191Glutathione; via amide nitrogen
Binding sitei370 – 3701ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi394 – 40512ATP By similarityAdd
BLAST

GO - Molecular functioni

  1. ATPase activity, coupled to transmembrane movement of substances Source: InterPro
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. ion transport Source: UniProtKB-KW
  2. response to mercury ion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Ion transport, Mercuric resistance, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNARO279238:GHBU-2695-MONOMER.

Protein family/group databases

TCDBi3.A.1.210.11. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ATM1-type heavy metal exporter
Alternative name(s):
ATP-binding cassette transporter Atm1
Short name:
NaAtm1
Gene namesi
Name:atm1
Ordered Locus Names:Saro_2631
OrganismiNovosphingobium aromaticivorans (strain DSM 12444 / F199)
Taxonomic identifieri279238 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium
ProteomesiUP000009134: Chromosome

Subcellular locationi

Cell inner membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3838Cytoplasmic1 PublicationAdd
BLAST
Transmembranei39 – 6022HelicalAdd
BLAST
Topological domaini61 – 8222Periplasmic1 PublicationAdd
BLAST
Transmembranei83 – 10523HelicalAdd
BLAST
Topological domaini106 – 15449Cytoplasmic1 PublicationAdd
BLAST
Transmembranei155 – 17824HelicalAdd
BLAST
Topological domaini179 – 1791Periplasmic1 Publication
Transmembranei180 – 20223HelicalAdd
BLAST
Topological domaini203 – 26664Cytoplasmic1 PublicationAdd
BLAST
Transmembranei267 – 28519HelicalAdd
BLAST
Topological domaini286 – 30015Periplasmic1 PublicationAdd
BLAST
Transmembranei301 – 32222HelicalAdd
BLAST
Topological domaini323 – 608286Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi195 – 1951Y → F: Strongly increases basal rate of ATP hydrolysis. 1 Publication
Mutagenesisi269 – 2691N → A: Increases basal rate of ATP hydrolysis and abolishes stimulation of ATP hydrolysis by glutathione. 1 Publication
Mutagenesisi272 – 2721Q → A: Abolishes glutathione-dependent ATP hydrolysis. 1 Publication
Mutagenesisi319 – 3191G → L: Abolishes glutathione-dependent ATP hydrolysis. 1 Publication
Mutagenesisi523 – 5231E → Q: Abolishes transporter activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608ATM1-type heavy metal exporterPRO_0000429375Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi279238.Saro_2631.

Structurei

Secondary structure

1
608
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 123
Helixi16 – 2712
Helixi34 – 6835
Turni70 – 723
Beta strandi73 – 753
Helixi76 – 12651
Helixi129 – 1313
Helixi137 – 16024
Helixi162 – 17918
Helixi183 – 22947
Helixi231 – 2366
Helixi240 – 29253
Helixi299 – 31214
Helixi314 – 3174
Helixi318 – 34023
Beta strandi361 – 3688
Beta strandi377 – 3848
Beta strandi389 – 3935
Helixi400 – 4023
Helixi403 – 4075
Beta strandi414 – 4207
Helixi425 – 4273
Helixi430 – 4356
Beta strandi437 – 4437
Beta strandi448 – 4503
Helixi451 – 4566
Helixi464 – 47310
Helixi477 – 4815
Beta strandi483 – 4853
Helixi486 – 4883
Beta strandi490 – 4923
Helixi500 – 51415
Beta strandi517 – 5237
Turni524 – 5274
Helixi530 – 54314
Turni544 – 5463
Beta strandi547 – 5526
Helixi556 – 5594
Beta strandi563 – 5697
Beta strandi572 – 5776
Helixi579 – 5857
Helixi588 – 60518

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4MRNX-ray2.50A/B1-608[»]
4MRPX-ray2.50A/B1-608[»]
4MRRX-ray2.97A/B1-608[»]
4MRSX-ray2.35A/B1-608[»]
4MRVX-ray2.50A/B1-608[»]
ProteinModelPortaliQ2G506.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 327289ABC transmembrane type-1Add
BLAST
Domaini361 – 595235ABC transporterAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni206 – 2105Glutathione binding
Regioni269 – 2724Glutathione binding

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5265.
KOiK06147.
OMAiFLPYLWP.
OrthoDBiEOG6T7N3V.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2G506-1 [UniParc]FASTAAdd to Basket

« Hide

MPPETATNPK DARHDGWQTL KRFLPYLWPA DNAVLRRRVV GAILMVLLGK    50
ATTLALPFAY KKAVDAMTLG GGAQPALTVA LAFVLAYALG RFSGVLFDNL 100
RNIVFERVGQ DATRHLAENV FARLHKLSLR FHLARRTGEV TKVIERGTKS 150
IDTMLYFLLF NIAPTVIELT AVIVIFWLNF GLGLVTATIL AVIAYVWTTR 200
TITEWRTHLR EKMNRLDGQA LARAVDSLLN YETVKYFGAE SREEARYASA 250
ARAYADAAVK SENSLGLLNI AQALIVNLLM AGAMAWTVYG WSQGKLTVGD 300
LVFVNTYLTQ LFRPLDMLGM VYRTIRQGLI DMAEMFRLID THIEVADVPN 350
APALVVNRPS VTFDNVVFGY DRDREILHGL SFEVAAGSRV AIVGPSGAGK 400
STIARLLFRF YDPWEGRILI DGQDIAHVTQ TSLRAALGIV PQDSVLFNDT 450
IGYNIAYGRD GASRAEVDAA AKGAAIADFI ARLPQGYDTE VGERGLKLSG 500
GEKQRVAIAR TLVKNPPILL FDEATSALDT RTEQDILSTM RAVASHRTTI 550
SIAHRLSTIA DSDTILVLDQ GRLAEQGSHL DLLRRDGLYA EMWARQAAES 600
AEVSEAAE 608
Length:608
Mass (Da):66,867
Last modified:March 21, 2006 - v1
Checksum:iC6D669186EFC501E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000248 Genomic DNA. Translation: ABD27067.1.
RefSeqiYP_497901.1. NC_007794.1.

Genome annotation databases

EnsemblBacteriaiABD27067; ABD27067; Saro_2631.
GeneIDi3917064.
KEGGinar:Saro_2631.
PATRICi22788070. VBINovAro50627_2694.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000248 Genomic DNA. Translation: ABD27067.1 .
RefSeqi YP_497901.1. NC_007794.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4MRN X-ray 2.50 A/B 1-608 [» ]
4MRP X-ray 2.50 A/B 1-608 [» ]
4MRR X-ray 2.97 A/B 1-608 [» ]
4MRS X-ray 2.35 A/B 1-608 [» ]
4MRV X-ray 2.50 A/B 1-608 [» ]
ProteinModelPortali Q2G506.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 279238.Saro_2631.

Protein family/group databases

TCDBi 3.A.1.210.11. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD27067 ; ABD27067 ; Saro_2631 .
GeneIDi 3917064.
KEGGi nar:Saro_2631.
PATRICi 22788070. VBINovAro50627_2694.

Phylogenomic databases

eggNOGi COG5265.
KOi K06147.
OMAi FLPYLWP.
OrthoDBi EOG6T7N3V.

Enzyme and pathway databases

BioCyci NARO279238:GHBU-2695-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEi PS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 12444 / F199.
  2. "Structural basis for heavy metal detoxification by an Atm1-type ABC exporter."
    Lee J.Y., Yang J.G., Zhitnitsky D., Lewinson O., Rees D.C.
    Science 343:1133-1136(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEXES WITH GLUTATHIONE AND PHOSPHATE, FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, TOPOLOGY, MUTAGENESIS OF TYR-195; ASN-269; GLN-272; GLY-319 AND GLU-523.
    Strain: DSM 12444 / F199.

Entry informationi

Entry nameiATM1_NOVAD
AccessioniPrimary (citable) accession number: Q2G506
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 11, 2014
Last sequence update: March 21, 2006
Last modified: September 3, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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