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Protein

ATM1-type heavy metal exporter

Gene

atm1

Organism
Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the ATP-dependent export of glutathione-conjugated substrates, such as heavy metal-glutathione conjugates. ATP hydrolysis is stimulated by glutathione binding. Protects cells against toxic heavy metal ions, such as silver and mercury ions. May also mediate the transport of glutathione-conjugated aromatic hydrocarbons, such as dinitrobenzene.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei319Glutathione; via amide nitrogen1
Binding sitei370ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi394 – 405ATPPROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Mercuric resistance, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.1.210.11. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ATM1-type heavy metal exporter
Alternative name(s):
ATP-binding cassette transporter Atm1
Short name:
NaAtm1
Gene namesi
Name:atm1
Ordered Locus Names:Saro_2631
OrganismiNovosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)
Taxonomic identifieri279238 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium
Proteomesi
  • UP000009134 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 38Cytoplasmic1 PublicationAdd BLAST38
Transmembranei39 – 60HelicalAdd BLAST22
Topological domaini61 – 82Periplasmic1 PublicationAdd BLAST22
Transmembranei83 – 105HelicalAdd BLAST23
Topological domaini106 – 154Cytoplasmic1 PublicationAdd BLAST49
Transmembranei155 – 178HelicalAdd BLAST24
Topological domaini179Periplasmic1 Publication1
Transmembranei180 – 202HelicalAdd BLAST23
Topological domaini203 – 266Cytoplasmic1 PublicationAdd BLAST64
Transmembranei267 – 285HelicalAdd BLAST19
Topological domaini286 – 300Periplasmic1 PublicationAdd BLAST15
Transmembranei301 – 322HelicalAdd BLAST22
Topological domaini323 – 608Cytoplasmic1 PublicationAdd BLAST286

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi195Y → F: Strongly increases basal rate of ATP hydrolysis. 1 Publication1
Mutagenesisi269N → A: Increases basal rate of ATP hydrolysis and abolishes stimulation of ATP hydrolysis by glutathione. 1 Publication1
Mutagenesisi272Q → A: Abolishes glutathione-dependent ATP hydrolysis. 1 Publication1
Mutagenesisi319G → L: Abolishes glutathione-dependent ATP hydrolysis. 1 Publication1
Mutagenesisi523E → Q: Abolishes transporter activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004293751 – 608ATM1-type heavy metal exporterAdd BLAST608

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-61368N.
STRINGi279238.Saro_2631.

Structurei

Secondary structure

1608
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 12Combined sources3
Helixi16 – 27Combined sources12
Helixi34 – 68Combined sources35
Turni70 – 72Combined sources3
Beta strandi73 – 75Combined sources3
Helixi76 – 126Combined sources51
Helixi129 – 131Combined sources3
Helixi137 – 160Combined sources24
Helixi162 – 179Combined sources18
Helixi183 – 229Combined sources47
Helixi231 – 236Combined sources6
Helixi240 – 292Combined sources53
Helixi299 – 312Combined sources14
Helixi314 – 317Combined sources4
Helixi318 – 340Combined sources23
Beta strandi361 – 368Combined sources8
Beta strandi377 – 384Combined sources8
Beta strandi389 – 393Combined sources5
Helixi400 – 402Combined sources3
Helixi403 – 407Combined sources5
Beta strandi414 – 420Combined sources7
Helixi425 – 427Combined sources3
Helixi430 – 435Combined sources6
Beta strandi437 – 443Combined sources7
Beta strandi448 – 450Combined sources3
Helixi451 – 456Combined sources6
Helixi464 – 473Combined sources10
Helixi477 – 481Combined sources5
Beta strandi483 – 485Combined sources3
Helixi486 – 488Combined sources3
Beta strandi490 – 492Combined sources3
Helixi500 – 514Combined sources15
Beta strandi517 – 523Combined sources7
Turni524 – 527Combined sources4
Helixi530 – 543Combined sources14
Turni544 – 546Combined sources3
Beta strandi547 – 552Combined sources6
Helixi556 – 559Combined sources4
Beta strandi563 – 569Combined sources7
Beta strandi572 – 577Combined sources6
Helixi579 – 585Combined sources7
Helixi588 – 605Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4MRNX-ray2.50A/B1-608[»]
4MRPX-ray2.50A/B1-608[»]
4MRRX-ray2.97A/B1-608[»]
4MRSX-ray2.35A/B1-608[»]
4MRVX-ray2.50A/B1-608[»]
ProteinModelPortaliQ2G506.
SMRiQ2G506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 327ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST289
Domaini361 – 595ABC transporterPROSITE-ProRule annotationAdd BLAST235

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni206 – 210Glutathione binding5
Regioni269 – 272Glutathione binding4

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108JJ7. Bacteria.
COG5265. LUCA.
KOiK06147.
OMAiDTDANQK.
OrthoDBiPOG091H00DB.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2G506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPETATNPK DARHDGWQTL KRFLPYLWPA DNAVLRRRVV GAILMVLLGK
60 70 80 90 100
ATTLALPFAY KKAVDAMTLG GGAQPALTVA LAFVLAYALG RFSGVLFDNL
110 120 130 140 150
RNIVFERVGQ DATRHLAENV FARLHKLSLR FHLARRTGEV TKVIERGTKS
160 170 180 190 200
IDTMLYFLLF NIAPTVIELT AVIVIFWLNF GLGLVTATIL AVIAYVWTTR
210 220 230 240 250
TITEWRTHLR EKMNRLDGQA LARAVDSLLN YETVKYFGAE SREEARYASA
260 270 280 290 300
ARAYADAAVK SENSLGLLNI AQALIVNLLM AGAMAWTVYG WSQGKLTVGD
310 320 330 340 350
LVFVNTYLTQ LFRPLDMLGM VYRTIRQGLI DMAEMFRLID THIEVADVPN
360 370 380 390 400
APALVVNRPS VTFDNVVFGY DRDREILHGL SFEVAAGSRV AIVGPSGAGK
410 420 430 440 450
STIARLLFRF YDPWEGRILI DGQDIAHVTQ TSLRAALGIV PQDSVLFNDT
460 470 480 490 500
IGYNIAYGRD GASRAEVDAA AKGAAIADFI ARLPQGYDTE VGERGLKLSG
510 520 530 540 550
GEKQRVAIAR TLVKNPPILL FDEATSALDT RTEQDILSTM RAVASHRTTI
560 570 580 590 600
SIAHRLSTIA DSDTILVLDQ GRLAEQGSHL DLLRRDGLYA EMWARQAAES

AEVSEAAE
Length:608
Mass (Da):66,867
Last modified:March 21, 2006 - v1
Checksum:iC6D669186EFC501E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000248 Genomic DNA. Translation: ABD27067.1.
RefSeqiWP_011446273.1. NC_007794.1.

Genome annotation databases

EnsemblBacteriaiABD27067; ABD27067; Saro_2631.
KEGGinar:Saro_2631.
PATRICi22788070. VBINovAro50627_2694.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000248 Genomic DNA. Translation: ABD27067.1.
RefSeqiWP_011446273.1. NC_007794.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4MRNX-ray2.50A/B1-608[»]
4MRPX-ray2.50A/B1-608[»]
4MRRX-ray2.97A/B1-608[»]
4MRSX-ray2.35A/B1-608[»]
4MRVX-ray2.50A/B1-608[»]
ProteinModelPortaliQ2G506.
SMRiQ2G506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61368N.
STRINGi279238.Saro_2631.

Protein family/group databases

TCDBi3.A.1.210.11. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD27067; ABD27067; Saro_2631.
KEGGinar:Saro_2631.
PATRICi22788070. VBINovAro50627_2694.

Phylogenomic databases

eggNOGiENOG4108JJ7. Bacteria.
COG5265. LUCA.
KOiK06147.
OMAiDTDANQK.
OrthoDBiPOG091H00DB.

Miscellaneous databases

PROiQ2G506.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATM1_NOVAD
AccessioniPrimary (citable) accession number: Q2G506
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 11, 2014
Last sequence update: March 21, 2006
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.