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Q2G506

- ATM1_NOVAD

UniProt

Q2G506 - ATM1_NOVAD

Protein

ATM1-type heavy metal exporter

Gene

atm1

Organism
Novosphingobium aromaticivorans (strain DSM 12444 / F199)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (21 Mar 2006)
      Previous versions | rss
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    Functioni

    Mediates the ATP-dependent export of glutathione-conjugated substrates, such as heavy metal-glutathione conjugates. ATP hydrolysis is stimulated by glutathione binding. Protects cells against toxic heavy metal ions, such as silver and mercury ions. May also mediate the transport of glutathione-conjugated aromatic hydrocarbons, such as dinitrobenzene.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei319 – 3191Glutathione; via amide nitrogen
    Binding sitei370 – 3701ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi394 – 40512ATPPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATPase activity, coupled to transmembrane movement of substances Source: InterPro
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. ion transport Source: UniProtKB-KW
    2. response to mercury ion Source: UniProtKB-KW

    Keywords - Biological processi

    Ion transport, Mercuric resistance, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciNARO279238:GHBU-2695-MONOMER.

    Protein family/group databases

    TCDBi3.A.1.210.11. the atp-binding cassette (abc) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATM1-type heavy metal exporter
    Alternative name(s):
    ATP-binding cassette transporter Atm1
    Short name:
    NaAtm1
    Gene namesi
    Name:atm1
    Ordered Locus Names:Saro_2631
    OrganismiNovosphingobium aromaticivorans (strain DSM 12444 / F199)
    Taxonomic identifieri279238 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium
    ProteomesiUP000009134: Chromosome

    Subcellular locationi

    Cell inner membrane 1 Publication; Multi-pass membrane protein 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi195 – 1951Y → F: Strongly increases basal rate of ATP hydrolysis. 1 Publication
    Mutagenesisi269 – 2691N → A: Increases basal rate of ATP hydrolysis and abolishes stimulation of ATP hydrolysis by glutathione. 1 Publication
    Mutagenesisi272 – 2721Q → A: Abolishes glutathione-dependent ATP hydrolysis. 1 Publication
    Mutagenesisi319 – 3191G → L: Abolishes glutathione-dependent ATP hydrolysis. 1 Publication
    Mutagenesisi523 – 5231E → Q: Abolishes transporter activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 608608ATM1-type heavy metal exporterPRO_0000429375Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi279238.Saro_2631.

    Structurei

    Secondary structure

    1
    608
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi10 – 123
    Helixi16 – 2712
    Helixi34 – 6835
    Turni70 – 723
    Beta strandi73 – 753
    Helixi76 – 12651
    Helixi129 – 1313
    Helixi137 – 16024
    Helixi162 – 17918
    Helixi183 – 22947
    Helixi231 – 2366
    Helixi240 – 29253
    Helixi299 – 31214
    Helixi314 – 3174
    Helixi318 – 34023
    Beta strandi361 – 3688
    Beta strandi377 – 3848
    Beta strandi389 – 3935
    Helixi400 – 4023
    Helixi403 – 4075
    Beta strandi414 – 4207
    Helixi425 – 4273
    Helixi430 – 4356
    Beta strandi437 – 4437
    Beta strandi448 – 4503
    Helixi451 – 4566
    Helixi464 – 47310
    Helixi477 – 4815
    Beta strandi483 – 4853
    Helixi486 – 4883
    Beta strandi490 – 4923
    Helixi500 – 51415
    Beta strandi517 – 5237
    Turni524 – 5274
    Helixi530 – 54314
    Turni544 – 5463
    Beta strandi547 – 5526
    Helixi556 – 5594
    Beta strandi563 – 5697
    Beta strandi572 – 5776
    Helixi579 – 5857
    Helixi588 – 60518

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4MRNX-ray2.50A/B1-608[»]
    4MRPX-ray2.50A/B1-608[»]
    4MRRX-ray2.97A/B1-608[»]
    4MRSX-ray2.35A/B1-608[»]
    4MRVX-ray2.50A/B1-608[»]
    ProteinModelPortaliQ2G506.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3838Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini61 – 8222Periplasmic1 PublicationAdd
    BLAST
    Topological domaini106 – 15449Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini179 – 1791Periplasmic1 Publication
    Topological domaini203 – 26664Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini286 – 30015Periplasmic1 PublicationAdd
    BLAST
    Topological domaini323 – 608286Cytoplasmic1 PublicationAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei39 – 6022HelicalAdd
    BLAST
    Transmembranei83 – 10523HelicalAdd
    BLAST
    Transmembranei155 – 17824HelicalAdd
    BLAST
    Transmembranei180 – 20223HelicalAdd
    BLAST
    Transmembranei267 – 28519HelicalAdd
    BLAST
    Transmembranei301 – 32222HelicalAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 327289ABC transmembrane type-1PROSITE-ProRule annotationAdd
    BLAST
    Domaini361 – 595235ABC transporterPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni206 – 2105Glutathione binding
    Regioni269 – 2724Glutathione binding

    Sequence similaritiesi

    Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
    Contains 1 ABC transporter domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5265.
    KOiK06147.
    OMAiFLPYLWP.
    OrthoDBiEOG6T7N3V.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00664. ABC_membrane. 1 hit.
    PF00005. ABC_tran. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF90123. SSF90123. 1 hit.
    PROSITEiPS50929. ABC_TM1F. 1 hit.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2G506-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPETATNPK DARHDGWQTL KRFLPYLWPA DNAVLRRRVV GAILMVLLGK    50
    ATTLALPFAY KKAVDAMTLG GGAQPALTVA LAFVLAYALG RFSGVLFDNL 100
    RNIVFERVGQ DATRHLAENV FARLHKLSLR FHLARRTGEV TKVIERGTKS 150
    IDTMLYFLLF NIAPTVIELT AVIVIFWLNF GLGLVTATIL AVIAYVWTTR 200
    TITEWRTHLR EKMNRLDGQA LARAVDSLLN YETVKYFGAE SREEARYASA 250
    ARAYADAAVK SENSLGLLNI AQALIVNLLM AGAMAWTVYG WSQGKLTVGD 300
    LVFVNTYLTQ LFRPLDMLGM VYRTIRQGLI DMAEMFRLID THIEVADVPN 350
    APALVVNRPS VTFDNVVFGY DRDREILHGL SFEVAAGSRV AIVGPSGAGK 400
    STIARLLFRF YDPWEGRILI DGQDIAHVTQ TSLRAALGIV PQDSVLFNDT 450
    IGYNIAYGRD GASRAEVDAA AKGAAIADFI ARLPQGYDTE VGERGLKLSG 500
    GEKQRVAIAR TLVKNPPILL FDEATSALDT RTEQDILSTM RAVASHRTTI 550
    SIAHRLSTIA DSDTILVLDQ GRLAEQGSHL DLLRRDGLYA EMWARQAAES 600
    AEVSEAAE 608
    Length:608
    Mass (Da):66,867
    Last modified:March 21, 2006 - v1
    Checksum:iC6D669186EFC501E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000248 Genomic DNA. Translation: ABD27067.1.
    RefSeqiYP_497901.1. NC_007794.1.

    Genome annotation databases

    EnsemblBacteriaiABD27067; ABD27067; Saro_2631.
    GeneIDi3917064.
    KEGGinar:Saro_2631.
    PATRICi22788070. VBINovAro50627_2694.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000248 Genomic DNA. Translation: ABD27067.1 .
    RefSeqi YP_497901.1. NC_007794.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4MRN X-ray 2.50 A/B 1-608 [» ]
    4MRP X-ray 2.50 A/B 1-608 [» ]
    4MRR X-ray 2.97 A/B 1-608 [» ]
    4MRS X-ray 2.35 A/B 1-608 [» ]
    4MRV X-ray 2.50 A/B 1-608 [» ]
    ProteinModelPortali Q2G506.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 279238.Saro_2631.

    Protein family/group databases

    TCDBi 3.A.1.210.11. the atp-binding cassette (abc) superfamily.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD27067 ; ABD27067 ; Saro_2631 .
    GeneIDi 3917064.
    KEGGi nar:Saro_2631.
    PATRICi 22788070. VBINovAro50627_2694.

    Phylogenomic databases

    eggNOGi COG5265.
    KOi K06147.
    OMAi FLPYLWP.
    OrthoDBi EOG6T7N3V.

    Enzyme and pathway databases

    BioCyci NARO279238:GHBU-2695-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00664. ABC_membrane. 1 hit.
    PF00005. ABC_tran. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF90123. SSF90123. 1 hit.
    PROSITEi PS50929. ABC_TM1F. 1 hit.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 12444 / F199.
    2. "Structural basis for heavy metal detoxification by an Atm1-type ABC exporter."
      Lee J.Y., Yang J.G., Zhitnitsky D., Lewinson O., Rees D.C.
      Science 343:1133-1136(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEXES WITH GLUTATHIONE AND PHOSPHATE, FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, TOPOLOGY, MUTAGENESIS OF TYR-195; ASN-269; GLN-272; GLY-319 AND GLU-523.
      Strain: DSM 12444 / F199.

    Entry informationi

    Entry nameiATM1_NOVAD
    AccessioniPrimary (citable) accession number: Q2G506
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 11, 2014
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3