Q2G3J8 (OTC_NOVAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase
Short name=OTCase
EC=2.1.3.3

Gene names

Name:	argF
Ordered Locus Names:	Saro_3140

Organism

Novosphingobium aromaticivorans (strain DSM 12444) [Complete proteome] [HAMAP]

Taxonomic identifier

279238 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Novosphingobium

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Subcellular location	Cytoplasm By similarity HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_1000213570
Regions
	Region	60 – 64	5	Carbamoyl phosphate binding By similarity
	Region	267 – 270	4	Ornithine binding By similarity
Sites
	Binding site	111	1	Carbamoyl phosphate By similarity
	Binding site	138	1	Carbamoyl phosphate By similarity
	Site	30	1	Important for structural integrity By similarity
	Site	151	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2G3J8 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: E49EA03D88BF112A	FASTA	311	33,086
	10 20 30 40 50 60 MSQALKHFLN LTDAGGDAIA AMLNDALDRK SARAGQPKGK ADADAPLGGR VLAMVFEKNS 70 80 90 100 110 120 TRTRVSFDIA MRQLGGSALI LDAGTTQLGR GETIADTARV LSRMADAIML RTDDHAKVEE 130 140 150 160 170 180 LARHATVPVI NGLTDLSHPC QIMADLLTVI EHGKALPGLE VAWLGDGNNV LNSIVEAAGL 190 200 210 220 230 240 MKFNVRIGVP EGYESDAGMI EAAVLAGAGI RVIRDPVEAV RGADVVVTDT WISMGQAHAE 250 260 270 280 290 300 AKLAAMAPYQ VNDALMAHAK PDAVFLHCLP AHRGEEVTDA VIDGPRSVVW DEAENRIHAQ 310 KSVLRWVFGQ I

References

[1]

"Complete sequence of Novosphingobium aromaticivorans DSM 12444." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. , Fredrickson J., Balkwill D., Romine M.F., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 12444.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000248 Genomic DNA. Translation: ABD27575.1.
RefSeq	YP_498409.1. NC_007794.1.
3D structure databases
HSSP	HSSP built from PDB template 1VLV based on UniProtKB P96108.
ProteinModelPortal	Q2G3J8.
SMR	Q2G3J8. Positions 1-311.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2G3J8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3918182.
GenomeReviews	Gene locus Saro_3140 in contig CP000248_GR.
KEGG	nar:Saro_3140.
NMPDR	fig|48935.1.peg.698.
PATRIC	22789136. VBINovAro50627_3220.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0078.
HOGENOM	HBG579429.
OMA	GVDCVVT.
PhylomeDB	Q2G3J8.
ProtClustDB	PRK00779.
Enzyme and pathway databases
BioCyc	NARO279238:SARO_3140-MONOMER.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_NOVAD

Accession

Primary (citable) accession number: Q2G3J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program