Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q2G3C3 (SYL_NOVAD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Saro_3215
OrganismNovosphingobium aromaticivorans (strain DSM 12444) [Complete proteome] [HAMAP]
Taxonomic identifier279238 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium

Protein attributes

Sequence length848 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 848848Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334784

Regions

Motif51 – 6111"HIGH" region HAMAP-Rule MF_00049
Motif625 – 6295"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6281ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2G3C3 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: D2C85B84C8D6C88B

FASTA84894,260
        10         20         30         40         50         60 
MTENTPGTSA PERFDPATAD TRWQRVWDEK QSFRADDSST KPRSYVLEMF PYPSGRIHIG 

        70         80         90        100        110        120 
HVRNYTMGDV LARYKRMRGF EVLHPMGWDA FGMPAENAAM EKGVHPGGWT RDNIANMKAQ 

       130        140        150        160        170        180 
LKRLGFALDW SREIATCEPE YYGQEQALFL DLYAAGLVYR KESTVNWDPV DMTVLANEQV 

       190        200        210        220        230        240 
IDGRGWRSGA LVEKRKLNQW FLKITDFADE LLEGLSTLDK WPEKVRVMQE NWIGKSQGLQ 

       250        260        270        280        290        300 
FRFDLSNGET VEVYTTRPDT IFGASFVAVA PDHPIAQGVA AINCEAANFI ALCKKGGTTA 

       310        320        330        340        350        360 
AELETAEKLG FDTGIGARHP LTGKYLPVYI ANFVLMEYGT GAIMAVPGHD QRDFDFATKY 

       370        380        390        400        410        420 
ALPILRVVAA DAADADKPFA GEAEAGDGVL VNSGFLDGMN VADAKAAVIV RAESEGWGEG 

       430        440        450        460        470        480 
KTVWRLRDWG VSRQRYWGTP IPFIHCEVCG VVPVPKKHLP VTLPEDVSFD VPGNPLDRHP 

       490        500        510        520        530        540 
TWKHVDCPQC GHPARRETDT LDTFVDSSWY FLRFASQPED RPFDPEEIKR WLPVEQYIGG 

       550        560        570        580        590        600 
IEHAILHLLY ARFWTRALAR IGKVEVTEPF GSLFTQGMVT HETYERRNPE NGQPIFFSPG 

       610        620        630        640        650        660 
EVERTGEGAT LKVDGAPVEI GRVIKMSKSK KNVVDPDEIV AKYGADAIRW FMLSDSPPER 

       670        680        690        700        710        720 
DLPWSEAGIE GCARFVQRLW RLFGQYDAAA TGEDKALDRK AHQTVHAVAS DIEALGFNKA 

       730        740        750        760        770        780 
VARIYELTGA VEKAAPSASR SDAIRKLLLL VAPMMPHLAE EAYARFGSSL IADAAWPEVD 

       790        800        810        820        830        840 
PALLVDDEVI VAVQVKGKLR DTLTVAKGTP KEDLERLALA SEKVQRALEG AEVKKVIVVP 


DRLVNLVA 

« Hide

References

[1]"Complete sequence of Novosphingobium aromaticivorans DSM 12444."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N. expand/collapse author list , Fredrickson J., Balkwill D., Romine M.F., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 12444.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000248 Genomic DNA. Translation: ABD27650.1.
RefSeqYP_498484.1. NC_007794.1.

3D structure databases

ProteinModelPortalQ2G3C3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING279238.Saro_3215.

Proteomic databases

PRIDEQ2G3C3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD27650; ABD27650; Saro_3215.
GeneID3917473.
KEGGnar:Saro_3215.
PATRIC22789292. VBINovAro50627_3298.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycNARO279238:GHBU-3287-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_NOVAD
AccessionPrimary (citable) accession number: Q2G3C3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 21, 2006
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries