Q2G2R8 (SSPP_STAA8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Staphopain A
EC=3.4.22.48

Alternative name(s):
Staphylococcal cysteine proteinase A
Staphylopain A

Gene names

Name:	sspP
Ordered Locus Names:	SAOUHSC_02127

Organism

Staphylococcus aureus (strain NCTC 8325)

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cysteine protease able to degrade elastin By similarity.
Catalytic activity	Broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate.
Enzyme regulation	Prematurely activated/folded staphopain A is inhibited by staphostatin A (scpB), which is probably required to protect staphylococcal cytoplasmic proteins from degradation by scpA By similarity.
Subunit structure	In the cytoplasm, prematurely activated/folded scpA forms a stable non-covalent complex with scpB By similarity.
Subcellular location	Secreted By similarity.
Induction	Expression occurs in a growth phase-dependent manner with optimal expression at post-exponential phase. Up-regulated by agr (accessory gene regulator) and repressed by sarA (staphylococcal accessory regulator) and sigmaB factor. Ref.2
Post-translational modification	Cleavage leads to the activation of scpA probably by an auto-catalytic manner By similarity.
Miscellaneous	The catalytic maturation of scpA appears to reside outside the cascade of activation started by the metalloprotease aureolysin (aur) By similarity.
Sequence similarities	Belongs to the peptidase C47 family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Propeptide	26 – 214	189	By similarity		PRO_0000247954
	Chain	215 – 388	174	Staphopain A		PRO_0000247955
Sites
	Active site	238	1	By similarity
	Active site	334	1	By similarity
	Active site	355	1	By similarity
	Site	214 – 215	2	Cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2G2R8 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 45D76DE341101F0D	FASTA	388	44,262
	10 20 30 40 50 60 MKRNFPKLIA LSLIFSLSVT PIANAESNSN IKAKDKKHVQ VNVEDKSVPT DVRNLAQKDY 70 80 90 100 110 120 LSYVTSLDKI YNKEKASYTL GEPFKIYKFN KKSDGNYYFP VLNTEGNIDY IVTISPKITK 130 140 150 160 170 180 YSSSSSKYTI NVSPFLSKVL NQYKDQQITI LTNSKGYYVV TQNHKAKLVL KTPRLEDKKL 190 200 210 220 230 240 KKTESIPTGN NVTQLKQKAS VTMPTSQFKS NNYTYNEQYI NKLENFKIRE TQGNNGWCAG 250 260 270 280 290 300 YTMSELLNAT YNTNKYHAEA VMRFLHPNLQ GQRFQFTGLT PREMIYFGQT QGRSPQLLNR 310 320 330 340 350 360 MTTYNEVDNL TKNNKGIAVL GSRVESRNGM HAGHAMAVVG NAKLDNGQEV IIIWNPWDNG 370 380 FMTQDAKNNV IPVSNGDHYR WYSSIYGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 8325.
[2]	"The role and regulation of the extracellular proteases of Staphylococcus aureus." Shaw L., Golonka E., Potempa J., Foster S.J. Microbiology 150:217-228(2004) [PubMed: 14702415] [Abstract] Cited for: PROTEIN SEQUENCE OF 215-222, INDUCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000253 Genomic DNA. Translation: ABD31176.1.
RefSeq	YP_500618.1. NC_007795.1.
3D structure databases
ProteinModelPortal	Q2G2R8.
SMR	Q2G2R8. Positions 216-388.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2G2R8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000030247; EBSTAP00000029198; EBSTAG00000030245.
GeneID	3921198.
GenomeReviews	Gene locus SAOUHSC_02127 in contig CP000253_GR.
KEGG	sao:SAOUHSC_02127.
PATRIC	19581617. VBIStaAur99865_1930.
Phylogenomic databases
eggNOG	NOG43315.
GeneTree	EBGT00050000023841.
HOGENOM	HBG217891.
OMA	YTINVSS.
ProtClustDB	CLSK885098.
Enzyme and pathway databases
BioCyc	SAUR93061:SAOUHSC_02127-MONOMER.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR008750. Peptidase_C47. [Graphical view]
KO	K08258.
Pfam	PF05543. Peptidase_C47. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. False negative. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SSPP_STAA8

Accession

Primary (citable) accession number: Q2G2R8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program