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Q2G2M6 (SYC_STAA8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cysteine--tRNA ligase

EC=6.1.1.16
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS
Gene names
Name:cysS
Ordered Locus Names:SAOUHSC_00511
OrganismStaphylococcus aureus (strain NCTC 8325) [Reference proteome] [HAMAP]
Taxonomic identifier93061 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00041

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00041

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00041.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcysteinyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

cysteine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466Cysteine--tRNA ligase HAMAP-Rule MF_00041
PRO_1000071077

Regions

Motif30 – 4011"HIGH" region HAMAP-Rule MF_00041
Motif265 – 2695"KMSKS" region HAMAP-Rule MF_00041

Sites

Metal binding281Zinc By similarity
Metal binding2081Zinc By similarity
Metal binding2331Zinc By similarity
Metal binding2371Zinc By similarity
Binding site2681ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2G2M6 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: 24875B53215743F7

FASTA46653,685
        10         20         30         40         50         60 
MITLYNTLTR QKEVFKPIEP GKVKMYVCGP TVYNYIHIGN ARPAINYDVV RRYFEYQGYN 

        70         80         90        100        110        120 
VEYVSNFTDV DDKLIKRSQE LNQSVPEIAE KYIAAFHEDV GALNVRKATS NPRVMDHMDD 

       130        140        150        160        170        180 
IIQFIKDLVD QGYAYESGGD VYFRTRKFEG YGKLSHQSID DLKVGARIDA GEHKEDALDF 

       190        200        210        220        230        240 
TLWKKAKPGE ISWDSPFGEG RPGWHIECSV MAFHELGPTI DIHAGGSDLQ FPHHENEIAQ 

       250        260        270        280        290        300 
SEAHNHAPFA NYWMHNGFIN IDNEKMSKSL GNFILVHDII KEVDPDVLRF FMISVHYRSP 

       310        320        330        340        350        360 
INYNLELVES ARSGLERIRN SYQLIEERAQ IATNIENQQT YIDQIDAILN RFETVMNDDF 

       370        380        390        400        410        420 
NTANAITAWY DLAKLANKYV LENTTSTEVI DKFKAVYQIF SDVLGVPLKS KNADELLDED 

       430        440        450        460 
VEKLIEERNE ARKNKDFARA DEIRDMLKSQ NIILEDTPQG VRFKRG 

« Hide

References

[1]"The Staphylococcus aureus NCTC8325 genome."
Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCTC 8325.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000253 Genomic DNA. Translation: ABD29660.1.
RefSeqYP_499084.1. NC_007795.1.

3D structure databases

ProteinModelPortalQ2G2M6.
SMRQ2G2M6. Positions 1-465.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING93061.SAOUHSC_00511.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD29660; ABD29660; SAOUHSC_00511.
GeneID3920423.
KEGGsao:SAOUHSC_00511.
PATRIC19578594. VBIStaAur99865_0457.

Phylogenomic databases

eggNOGCOG0215.
HOGENOMHOG000245250.
KOK01883.
OMAQIDIHAG.
OrthoDBEOG6RVFXC.
ProtClustDBPRK00260.

Enzyme and pathway databases

BioCycSAUR93061:GIWJ-494-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00041. Cys_tRNA_synth.
InterProIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERPTHR10890. PTHR10890. 1 hit.
PfamPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSPR00983. TRNASYNTHCYS.
SMARTSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
TIGRFAMsTIGR00435. cysS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYC_STAA8
AccessionPrimary (citable) accession number: Q2G2M6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 21, 2006
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries