Q2G261 (SODM2_STAA8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Superoxide dismutase [Mn/Fe] 2
EC=1.15.1.1

Gene names

Name:	sodM
Ordered Locus Names:	SAOUHSC_00093

Organism

Staphylococcus aureus (strain NCTC 8325) [Reference proteome] [HAMAP]

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. May play a role in maintaining cell viability during the late-exponential and stationary phases of growth since it becomes a major source of activity under oxidative stress. Has a role in resisting external superoxide stress. Involved in acid tolerance and the acid-adaptive response. Mediates the derepression of perR regulon in the response to HOCl stress at low level of SOD activity By similarity. Ref.1 Ref.5
Catalytic activity	2 superoxide + 2 H+ = O2 + H2O2.
Cofactor	Binds 1 manganese or iron ion per subunit By similarity.
Subunit structure	Homodimer. Can also form a heterodimer with SodA. Ref.4
Induction	Transcriptionally induced by externally generated superoxide stress in a manganese-dependent way. The presence of manganese increases SodA homodimer activity and simultaneously decreases SodM homodimer activity. This occurs primarily due to post-transcriptional effects, since the expression of the gene is independent of manganese availability in the absence of superoxide generating compounds. Ref.5
Miscellaneous	According to Ref.1 the levels of SodM activity and sodM expression are growth-phase dependent, occurring most during the late-exponential and stationary phases. This response is also dependent on the level of aeration with highest activity occurring under high aeration. SodM expression under low-aeration growth conditions is most abundant during the late-exponential phase while under high-aeration growth conditions is highest during the stationary phase. Transcribed from a single sigmaA-type promoter (PM). Transcriptional data show an indirect repression of PM promoter by sigmaB which can also be involved in the post-transcriptional regulation of SodM homodimer activity.
Sequence similarities	Belongs to the iron/manganese superoxide dismutase family.

Ontologies

Keywords
Biological process	Stress response
Ligand	Iron Manganese Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW superoxide metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW superoxide dismutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 199	199	Superoxide dismutase [Mn/Fe] 2		PRO_0000293965
Sites
	Metal binding	27	1	Manganese or iron By similarity
	Metal binding	81	1	Manganese or iron By similarity
	Metal binding	161	1	Manganese or iron By similarity
	Metal binding	165	1	Manganese or iron By similarity
Experimental info
	Sequence conflict	61	1	D → G in AAG44813. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q2G261 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 388566FB9943C635	FASTA	199	23,041
	10 20 30 40 50 60 MAFKLPNLPY AYDALEPYID QRTMEFHHDK HHNTYVTKLN ATVEGTELEH QSLADMIANL 70 80 90 100 110 120 DKVPEAMRMS VRNNGGGHFN HSLFWEILSP NSEEKGGVID DIKAQWGTLD EFKNEFANKA 130 140 150 160 170 180 TTLFGSGWTW LVVNDGKLEI VTTPNQDNPL TEGKTPILLF DVWEHAYYLK YQNKRPDYMT 190 AFWNIVNWKK VDELYQAAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a second superoxide dismutase gene (sodM) from Staphylococcus aureus." Wright Valderas M., Hart M.E. J. Bacteriol. 183:3399-3407(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, EXPRESSION, SOD ACTIVITY.
[2]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 8325.
[3]	"Characterization of superoxide dismutase genes from Gram-positive bacteria by polymerase chain reaction using degenerate primers." Poyart C., Berche P., Trieu-Cuot P. FEMS Microbiol. Lett. 131:41-45(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 18-160.
[4]	"The superoxide dismutase gene sodM is unique to Staphylococcus aureus: absence of sodM in coagulase-negative staphylococci." Wright Valderas M., Gatson J.W., Wreyford N., Hart M.E. J. Bacteriol. 184:2465-2472(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SOD ACTIVITY, SUBUNIT.
[5]	"Role and regulation of the superoxide dismutases of Staphylococcus aureus." Karavolos M.H., Horsburgh M.J., Ingham E., Foster S.J. Microbiology 149:2749-2758(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN OXIDATIVE STRESS RESISTANCE, EXPRESSION, REGULATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF273269 Genomic DNA. Translation: AAG44813.2. CP000253 Genomic DNA. Translation: ABD29276.1. Z49245 Genomic DNA. Translation: CAA89212.1.
PIR	S54793.
RefSeq	YP_498694.1. NC_007795.1.
3D structure databases
ProteinModelPortal	Q2G261.
SMR	Q2G261. Positions 3-198.
ModBase	Search...
Protein-protein interaction databases
STRING	93061.SAOUHSC_00093.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD29276; ABD29276; SAOUHSC_00093.
GeneID	3919804.
KEGG	sao:SAOUHSC_00093.
PATRIC	19577814. VBIStaAur99865_0083.
Phylogenomic databases
eggNOG	COG0605.
HOGENOM	HOG000013583.
KO	K04564.
OMA	KAWWNTV.
ProtClustDB	CLSK884424.
Enzyme and pathway databases
BioCyc	SAUR93061:GIWJ-88-MONOMER.
Family and domain databases
InterPro	IPR001189. Mn/Fe_SOD. IPR019833. Mn/Fe_SOD_BS. IPR019832. Mn/Fe_SOD_C. IPR019831. Mn/Fe_SOD_N. [Graphical view]
PANTHER	PTHR11404. PTHR11404. 1 hit.
Pfam	PF02777. Sod_Fe_C. 1 hit. PF00081. Sod_Fe_N. 1 hit. [Graphical view]
PIRSF	PIRSF000349. SODismutase. 1 hit.
PRINTS	PR01703. MNSODISMTASE.
SUPFAM	SSF46609. SODismutase. 1 hit. SSF54719. SODismutase. 1 hit.
PROSITE	PS00088. SOD_MN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SODM2_STAA8

Accession

Primary (citable) accession number: Q2G261
Secondary accession number(s): Q59806, Q9EZZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 21, 2006
Last modified:	May 29, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program