Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Superoxide dismutase [Mn/Fe] 2

Gene

sodM

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. May play a role in maintaining cell viability during the late-exponential and stationary phases of growth since it becomes a major source of activity under oxidative stress. Has a role in resisting external superoxide stress. Involved in acid tolerance and the acid-adaptive response. Mediates the derepression of perR regulon in the response to HOCl stress at low level of SOD activity (By similarity).By similarity

Catalytic activityi

2 superoxide + 2 H+ = O2 + H2O2.

Cofactori

Mn2+By similarity, Fe2+By similarityNote: Binds 1 Mn(2+) or Fe2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi27 – 271Manganese or ironBy similarity
Metal bindingi81 – 811Manganese or ironBy similarity
Metal bindingi161 – 1611Manganese or ironBy similarity
Metal bindingi165 – 1651Manganese or ironBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. superoxide dismutase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

Iron, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-88-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Superoxide dismutase [Mn/Fe] 2 (EC:1.15.1.1)
Gene namesi
Name:sodM
Ordered Locus Names:SAOUHSC_00093
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000008816 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 199199Superoxide dismutase [Mn/Fe] 2PRO_0000293965Add
BLAST

Expressioni

Inductioni

Transcriptionally induced by externally generated superoxide stress in a manganese-dependent way. The presence of manganese increases SodA homodimer activity and simultaneously decreases SodM homodimer activity. This occurs primarily due to post-transcriptional effects, since the expression of the gene is independent of manganese availability in the absence of superoxide generating compounds.

Interactioni

Subunit structurei

Homodimer. Can also form a heterodimer with SodA.1 Publication

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00093.

Structurei

3D structure databases

ProteinModelPortaliQ2G261.
SMRiQ2G261. Positions 3-198.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0605.
HOGENOMiHOG000013583.
KOiK04564.
OMAiGGGNKLP.
OrthoDBiEOG63NMNT.

Family and domain databases

InterProiIPR001189. Mn/Fe_SOD.
IPR019833. Mn/Fe_SOD_BS.
IPR019832. Mn/Fe_SOD_C.
IPR019831. Mn/Fe_SOD_N.
[Graphical view]
PANTHERiPTHR11404. PTHR11404. 1 hit.
PfamiPF02777. Sod_Fe_C. 1 hit.
PF00081. Sod_Fe_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000349. SODismutase. 1 hit.
PRINTSiPR01703. MNSODISMTASE.
SUPFAMiSSF46609. SSF46609. 1 hit.
SSF54719. SSF54719. 1 hit.
PROSITEiPS00088. SOD_MN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2G261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFKLPNLPY AYDALEPYID QRTMEFHHDK HHNTYVTKLN ATVEGTELEH
60 70 80 90 100
QSLADMIANL DKVPEAMRMS VRNNGGGHFN HSLFWEILSP NSEEKGGVID
110 120 130 140 150
DIKAQWGTLD EFKNEFANKA TTLFGSGWTW LVVNDGKLEI VTTPNQDNPL
160 170 180 190
TEGKTPILLF DVWEHAYYLK YQNKRPDYMT AFWNIVNWKK VDELYQAAK
Length:199
Mass (Da):23,041
Last modified:March 21, 2006 - v1
Checksum:i388566FB9943C635
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611D → G in AAG44813 (PubMed:11344148).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF273269 Genomic DNA. Translation: AAG44813.2.
CP000253 Genomic DNA. Translation: ABD29276.1.
Z49245 Genomic DNA. Translation: CAA89212.1.
PIRiS54793.
RefSeqiYP_498694.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD29276; ABD29276; SAOUHSC_00093.
GeneIDi3919804.
KEGGisao:SAOUHSC_00093.
PATRICi19577814. VBIStaAur99865_0083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF273269 Genomic DNA. Translation: AAG44813.2.
CP000253 Genomic DNA. Translation: ABD29276.1.
Z49245 Genomic DNA. Translation: CAA89212.1.
PIRiS54793.
RefSeqiYP_498694.1. NC_007795.1.

3D structure databases

ProteinModelPortaliQ2G261.
SMRiQ2G261. Positions 3-198.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD29276; ABD29276; SAOUHSC_00093.
GeneIDi3919804.
KEGGisao:SAOUHSC_00093.
PATRICi19577814. VBIStaAur99865_0083.

Phylogenomic databases

eggNOGiCOG0605.
HOGENOMiHOG000013583.
KOiK04564.
OMAiGGGNKLP.
OrthoDBiEOG63NMNT.

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-88-MONOMER.

Family and domain databases

InterProiIPR001189. Mn/Fe_SOD.
IPR019833. Mn/Fe_SOD_BS.
IPR019832. Mn/Fe_SOD_C.
IPR019831. Mn/Fe_SOD_N.
[Graphical view]
PANTHERiPTHR11404. PTHR11404. 1 hit.
PfamiPF02777. Sod_Fe_C. 1 hit.
PF00081. Sod_Fe_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000349. SODismutase. 1 hit.
PRINTSiPR01703. MNSODISMTASE.
SUPFAMiSSF46609. SSF46609. 1 hit.
SSF54719. SSF54719. 1 hit.
PROSITEiPS00088. SOD_MN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a second superoxide dismutase gene (sodM) from Staphylococcus aureus."
    Wright Valderas M., Hart M.E.
    J. Bacteriol. 183:3399-3407(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, EXPRESSION, SOD ACTIVITY.
  2. "The Staphylococcus aureus NCTC 8325 genome."
    Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
    (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 8325.
  3. "Characterization of superoxide dismutase genes from Gram-positive bacteria by polymerase chain reaction using degenerate primers."
    Poyart C., Berche P., Trieu-Cuot P.
    FEMS Microbiol. Lett. 131:41-45(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 18-160.
  4. "The superoxide dismutase gene sodM is unique to Staphylococcus aureus: absence of sodM in coagulase-negative staphylococci."
    Wright Valderas M., Gatson J.W., Wreyford N., Hart M.E.
    J. Bacteriol. 184:2465-2472(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SOD ACTIVITY, SUBUNIT.
  5. "Role and regulation of the superoxide dismutases of Staphylococcus aureus."
    Karavolos M.H., Horsburgh M.J., Ingham E., Foster S.J.
    Microbiology 149:2749-2758(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN OXIDATIVE STRESS RESISTANCE, EXPRESSION, REGULATION.

Entry informationi

Entry nameiSODM2_STAA8
AccessioniPrimary (citable) accession number: Q2G261
Secondary accession number(s): Q59806, Q9EZZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 21, 2006
Last modified: January 7, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

According to PubMed:11344148 the levels of SodM activity and sodM expression are growth-phase dependent, occurring most during the late-exponential and stationary phases. This response is also dependent on the level of aeration with highest activity occurring under high aeration. SodM expression under low-aeration growth conditions is most abundant during the late-exponential phase while under high-aeration growth conditions is highest during the stationary phase.
Transcribed from a single sigmaA-type promoter (PM). Transcriptional data show an indirect repression of PM promoter by sigmaB which can also be involved in the post-transcriptional regulation of SodM homodimer activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.