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Protein

Heme oxygenase (staphylobilin-producing) 2

Gene

isdI

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the oxo-bilirubin chromophore staphylobilin (a mixture of the linear tetrapyrroles 5-oxo-delta-bilirubin and 15-oxo-beta-bilirubin) in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron.UniRule annotation1 Publication

Catalytic activityi

Protoheme + 5 reduced acceptor + 4 O2 = 5-oxo-delta-bilirubin + Fe2+ + formaldehyde + 5 acceptor + 4 H2O.UniRule annotation1 Publication
Protoheme + 5 reduced acceptor + 4 O2 = 15-oxo-beta-bilirubin + Fe2+ + formaldehyde + 5 acceptor + 4 H2O.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi6IronUniRule annotation1
Sitei66Transition state stabilizerUniRule annotation1
Metal bindingi76Iron (heme axial ligand)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heme oxygenase (staphylobilin-producing) 2UniRule annotation (EC:1.14.99.48UniRule annotation1 Publication)
Alternative name(s):
Heme-degrading monooxygenase 2UniRule annotation
Iron-regulated surface determinant 2UniRule annotation
Iron-responsive surface determinant 2UniRule annotation
Gene namesi
Name:isdI
Ordered Locus Names:SAOUHSC_00130
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002700941 – 108Heme oxygenase (staphylobilin-producing) 2Add BLAST108

Expressioni

Inductioni

Transcriptionally regulated by iron and the ferric uptake repressor (fur) protein.Curated

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00130.

Structurei

3D structure databases

ProteinModelPortaliQ2G1J2.
SMRiQ2G1J2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 93ABMUniRule annotationAdd BLAST92

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 28Heme bindingUniRule annotation8

Sequence similaritiesi

Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase IsdG subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105Z26. Bacteria.
COG2329. LUCA.
HOGENOMiHOG000008026.
KOiK07145.
OMAiFRESHSH.

Family and domain databases

HAMAPiMF_01272. Heme_degrading_monooxygenase. 1 hit.
InterProiView protein in InterPro
IPR007138. ABM_dom.
IPR011008. Dimeric_a/b-barrel.
IPR023953. IsdG.
PfamiView protein in Pfam
PF03992. ABM. 1 hit.
SUPFAMiSSF54909. SSF54909. 1 hit.
PROSITEiView protein in PROSITE
PS51725. ABM. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2G1J2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFMAENRLQL QKGSAEETIE RFYNRQGIET IEGFQQMFVT KTLNTEDTDE
60 70 80 90 100
VKILTIWESE DSFNNWLNSD VFKEAHKNVR LKSDDDGQQS PILSNKVFKY

DIGYHYQK
Length:108
Mass (Da):12,791
Last modified:March 21, 2006 - v1
Checksum:i8AF2718571451004
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD29311.1.
RefSeqiWP_000480603.1. NC_007795.1.
YP_498730.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD29311; ABD29311; SAOUHSC_00130.
GeneIDi31212972.
3919839.
KEGGisao:SAOUHSC_00130.
PATRICifig|93061.5.peg.122.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiHDOX2_STAA8
AccessioniPrimary (citable) accession number: Q2G1J2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: March 21, 2006
Last modified: June 7, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families