Q2G1J2 (ISDI_STAA8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heme-degrading monooxygenase isdI
EC=1.14.99.3

Alternative name(s):
Heme oxygenase
Iron-regulated surface determinant isdI
Iron-responsive surface determinant isdI

Gene names

Name:	isdI
Ordered Locus Names:	SAOUHSC_00130

Organism

Staphylococcus aureus (strain NCTC 8325)

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron. Ref.2
Catalytic activity	Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O. Ref.2
Subunit structure	Homodimer By similarity. HAMAP MF_01272
Subcellular location	Cytoplasm Probable HAMAP MF_01272.
Induction	Transcriptionally regulated by iron and the ferric uptake repressor (fur) protein Probable. HAMAP MF_01272
Sequence similarities	Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase isdG subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	iron assimilation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme oxygenase (decyclizing) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 108	108	Heme-degrading monooxygenase isdI HAMAP MF_01272		PRO_0000270094
Sites
	Metal binding	6	1	Iron Potential
	Metal binding	76	1	Iron (heme axial ligand) Potential
	Site	66	1	Transition state stabilizer Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2G1J2 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 8AF2718571451004	FASTA	108	12,791
	10 20 30 40 50 60 MFMAENRLQL QKGSAEETIE RFYNRQGIET IEGFQQMFVT KTLNTEDTDE VKILTIWESE 70 80 90 100 DSFNNWLNSD VFKEAHKNVR LKSDDDGQQS PILSNKVFKY DIGYHYQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 8325.
[2]	"IsdG and IsdI, heme-degrading enzymes in the cytoplasm of Staphylococcus aureus." Skaar E.P., Gaspar A.H., Schneewind O. J. Biol. Chem. 279:436-443(2004) [PubMed: 14570922] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000253 Genomic DNA. Translation: ABD29311.1.
RefSeq	YP_498730.1. NC_007795.1.
3D structure databases
ProteinModelPortal	Q2G1J2.
SMR	Q2G1J2. Positions 1-108.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2G1J2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000030574; EBSTAP00000029525; EBSTAG00000030572.
GeneID	3919839.
GenomeReviews	Gene locus SAOUHSC_00130 in contig CP000253_GR.
KEGG	sao:SAOUHSC_00130.
PATRIC	19577892. VBIStaAur99865_0122.
Phylogenomic databases
eggNOG	COG2329.
GeneTree	EBGT00050000024362.
HOGENOM	HBG616589.
OMA	WTKSSAF.
ProtClustDB	PRK13313.
Enzyme and pathway databases
BioCyc	SAUR93061:SAOUHSC_00130-MONOMER.
Family and domain databases
HAMAP	MF_01272. IsdG. [Tree]
InterPro	IPR007138. Antibiotic_mOase. IPR011008. Dimeric_a/b-barrel. IPR023953. Heme-degrad_mOase. [Graphical view]
KO	K07145.
Pfam	PF03992. ABM. 1 hit. [Graphical view]
SUPFAM	SSF54909. Dimer_A_B_barrel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISDI_STAA8

Accession

Primary (citable) accession number: Q2G1J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program