Reviewed, UniProtKB/Swiss-Prot Q2G1J2 (ISDI_STAA8)

Names and origin

Protein names

Recommended name:
    Heme-degrading monooxygenase isdI
    EC=1.14.99.3
Alternative name(s):
    Iron-regulated surface determinant isdI
    Iron-responsive surface determinant isdI
    Heme oxygenase

Gene names

Name:	isdI
Ordered Locus Names:	SAOUHSC_00130

Organism

Staphylococcus aureus (strain NCTC 8325) [Complete proteome] [HAMAP]

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron. Ref.2
Catalytic activity	Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O. Ref.2
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Induction	Transcriptionally regulated by iron and the ferric uptake repressor (fur) protein Probable.
Sequence similarities	Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase isdG subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: HAMAP heme oxygenase (decyclizing) activity Inferred from electronic annotation. Source: EC monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 108	108	Heme-degrading monooxygenase isdI HAMAP MF_01272		PRO_0000270094
Sites
	Metal binding	6	1	Iron Potential
	Metal binding	76	1	Iron (heme axial ligand) Potential
	Site	66	1	Transition state stabilizer Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2G1J2-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 8AF2718571451004	FASTA	108	12,791
	10 20 30 40 50 60 MFMAENRLQL QKGSAEETIE RFYNRQGIET IEGFQQMFVT KTLNTEDTDE VKILTIWESE 70 80 90 100 DSFNNWLNSD VFKEAHKNVR LKSDDDGQQS PILSNKVFKY DIGYHYQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"IsdG and IsdI, heme-degrading enzymes in the cytoplasm of Staphylococcus aureus." Skaar E.P., Gaspar A.H., Schneewind O. J. Biol. Chem. 279:436-443(2004) [PubMed: 14570922] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000253 Genomic DNA. Translation: ABD29311.1.
RefSeq	YP_498730.1.
3D structure databases
SMR	Q2G1J2. Positions 1-108.
ModBase	Search...
Genome annotation databases
GeneID	3919839.
GenomeReviews	Gene locus SAOUHSC_00130 in contig CP000253_GR.
KEGG	sao:SAOUHSC_00130.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2G1J2.
OMA	Q2G1J2. DAHSHQG.
Enzyme and pathway databases
BioCyc	SAUR93061:SAOUHSC_00130-MON.
Family and domain databases
HAMAP	MF_01272. [Tree]
InterPro	IPR007138. Antibiotic_mOase. [Graphical view]
Pfam	PF03992. ABM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISDI_STAA8

Accession

Primary (citable) accession number: Q2G1J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)