Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyruvate formate-lyase-activating enzyme

Gene

pflA

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi29Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi33Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi36Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Binding sitei79S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei207S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
Short name:
PFL-activating enzyme
Gene namesi
Name:pflA
Ordered Locus Names:SAOUHSC_00188
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002717091 – 251Pyruvate formate-lyase-activating enzymeAdd BLAST251

Interactioni

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00188.

Structurei

3D structure databases

ProteinModelPortaliQ2G1D7.
SMRiQ2G1D7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 37S-adenosyl-L-methionine bindingBy similarity3
Regioni134 – 136S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105F1A. Bacteria.
COG1180. LUCA.
HOGENOMiHOG000011458.
KOiK04069.
OMAiMGVYKWE.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2G1D7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKGHLHSVE SLGTVDGPGL RYILFTQGCL LRCLYCHNPD TWKISEPSRE
60 70 80 90 100
VTVDEMVNEI LPYKPYFDAS GGGVTVSGGE PLLQMPFLEK LFAELKENGV
110 120 130 140 150
HTCLDTSAGC ANDTKAFQRH FEELQKHTDL ILLDIKHIDN DKHIRLTGKP
160 170 180 190 200
NTHILNFARK LSDMKQPVWI RHVLVPGYSD DKDDLIKLGE FINSLDNVEK
210 220 230 240 250
FEILPYHQLG VHKWKTLGIA YELEDVEAPD DEAVKAAYRY VNFKGKIPVE

L
Length:251
Mass (Da):28,499
Last modified:March 21, 2006 - v1
Checksum:i892A603E273F6C89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD29366.1.
RefSeqiWP_000911657.1. NC_007795.1.
YP_498785.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD29366; ABD29366; SAOUHSC_00188.
GeneIDi28379462.
3919502.
KEGGisao:SAOUHSC_00188.
PATRICi19578000. VBIStaAur99865_0176.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD29366.1.
RefSeqiWP_000911657.1. NC_007795.1.
YP_498785.1. NC_007795.1.

3D structure databases

ProteinModelPortaliQ2G1D7.
SMRiQ2G1D7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD29366; ABD29366; SAOUHSC_00188.
GeneIDi28379462.
3919502.
KEGGisao:SAOUHSC_00188.
PATRICi19578000. VBIStaAur99865_0176.

Phylogenomic databases

eggNOGiENOG4105F1A. Bacteria.
COG1180. LUCA.
HOGENOMiHOG000011458.
KOiK04069.
OMAiMGVYKWE.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFLA_STAA8
AccessioniPrimary (citable) accession number: Q2G1D7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: March 21, 2006
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.